CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

Background: Chemoresistance is a major barrier to the treatment of human cancers. Circular RNAs (circRNAs) are implicated in drug resistance in cancers, including gastric cancer (GC). In this study, we aimed to explore the functions of circRNA Armadillo Repeat gene deleted in Velo-Cardio-Facial synd...

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Autores principales:	Ruirui Zhang, Huanyu Zhao, Hongmei Yuan, Jian Wu, Haiyan Liu, Suan Sun, Zhengwei Zhang, Jiayang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	GC DDP resistance circARVCF miR-1205 FGFR1 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/21c4b1cf5ee84b2ca87038a76320c4ca
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spelling	oai:doaj.org-article:21c4b1cf5ee84b2ca87038a76320c4ca2021-12-01T02:26:05ZCircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR11664-802110.3389/fgene.2021.767590https://doaj.org/article/21c4b1cf5ee84b2ca87038a76320c4ca2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.767590/fullhttps://doaj.org/toc/1664-8021Background: Chemoresistance is a major barrier to the treatment of human cancers. Circular RNAs (circRNAs) are implicated in drug resistance in cancers, including gastric cancer (GC). In this study, we aimed to explore the functions of circRNA Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (circARVCF) in cisplatin (DDP) resistance in GC.Methods: The expression of circARVCF, microRNA-1205 (miR-1205) and fibroblast growth factor receptor 1 (FGFR1) was detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blot assay or immunohistochemistry (IHC) assay. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were performed to evaluate DDP resistance and cell colony formation ability. Transwell assay was conducted to assess cell migration and invasion. Flow cytometry analysis was done to analyze cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were manipulated to analyze the relationships of circARVCF, miR-1205 and FGFR1. Murine xenograft model was constructed to explore DDP resistance in vivo.Results: CircARVCF level was increased in DDP-resistant GC tissues and cells. CircARVCF silencing inhibited DDP resistance, colony formation and metastasis and induced apoptosis in DDP-resistant GC cells. CircARVCF directly interacted with miR-1205 and miR-1205 inhibition reversed circARVCF silencing-mediated effect on DDP resistance in DDP-resistant GC cells. FGFR1 served as the target gene of miR-1205. MiR-1205 overexpression restrained the resistance of DDP-resistant GC cells to DDP, but FGFR1 elevation abated the effect. In addition, circARVCF knockdown repressed DDP resistance in vivo.Conclusion: CircARVCF enhanced DDP resistance in GC by elevating FGFR1 through sponging miR-1205.Ruirui ZhangHuanyu ZhaoHongmei YuanJian WuHaiyan LiuSuan SunZhengwei ZhangJiayang WangFrontiers Media S.A.articleGCDDPresistancecircARVCFmiR-1205FGFR1GeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	GC DDP resistance circARVCF miR-1205 FGFR1 Genetics QH426-470
spellingShingle	GC DDP resistance circARVCF miR-1205 FGFR1 Genetics QH426-470 Ruirui Zhang Huanyu Zhao Hongmei Yuan Jian Wu Haiyan Liu Suan Sun Zhengwei Zhang Jiayang Wang CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
description	Background: Chemoresistance is a major barrier to the treatment of human cancers. Circular RNAs (circRNAs) are implicated in drug resistance in cancers, including gastric cancer (GC). In this study, we aimed to explore the functions of circRNA Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (circARVCF) in cisplatin (DDP) resistance in GC.Methods: The expression of circARVCF, microRNA-1205 (miR-1205) and fibroblast growth factor receptor 1 (FGFR1) was detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blot assay or immunohistochemistry (IHC) assay. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were performed to evaluate DDP resistance and cell colony formation ability. Transwell assay was conducted to assess cell migration and invasion. Flow cytometry analysis was done to analyze cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were manipulated to analyze the relationships of circARVCF, miR-1205 and FGFR1. Murine xenograft model was constructed to explore DDP resistance in vivo.Results: CircARVCF level was increased in DDP-resistant GC tissues and cells. CircARVCF silencing inhibited DDP resistance, colony formation and metastasis and induced apoptosis in DDP-resistant GC cells. CircARVCF directly interacted with miR-1205 and miR-1205 inhibition reversed circARVCF silencing-mediated effect on DDP resistance in DDP-resistant GC cells. FGFR1 served as the target gene of miR-1205. MiR-1205 overexpression restrained the resistance of DDP-resistant GC cells to DDP, but FGFR1 elevation abated the effect. In addition, circARVCF knockdown repressed DDP resistance in vivo.Conclusion: CircARVCF enhanced DDP resistance in GC by elevating FGFR1 through sponging miR-1205.
format	article
author	Ruirui Zhang Huanyu Zhao Hongmei Yuan Jian Wu Haiyan Liu Suan Sun Zhengwei Zhang Jiayang Wang
author_facet	Ruirui Zhang Huanyu Zhao Hongmei Yuan Jian Wu Haiyan Liu Suan Sun Zhengwei Zhang Jiayang Wang
author_sort	Ruirui Zhang
title	CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
title_short	CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
title_full	CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
title_fullStr	CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
title_full_unstemmed	CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1
title_sort	circarvcf contributes to cisplatin resistance in gastric cancer by altering mir-1205 and fgfr1
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/21c4b1cf5ee84b2ca87038a76320c4ca
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CircARVCF Contributes to Cisplatin Resistance in Gastric Cancer by Altering miR-1205 and FGFR1

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