Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics

Abstract Electrospun fibrous scaffolds combined with bioactive factors can display impressive performance as an ideal wound dressing, since they can mimic the composition and physicochemical properties of the extracellular matrix (ECM). The aim of this study was to fabricate a new composite biomater...

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Autores principales:	Jianhang Jiao, Chuangang Peng, Chen Li, Zhiping Qi, Jing Zhan, Su Pan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21cb104db5ac4bb897f5e061cc4c9fef
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spelling	oai:doaj.org-article:21cb104db5ac4bb897f5e061cc4c9fef2021-12-02T14:01:36ZDual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics10.1038/s41598-020-80269-22045-2322https://doaj.org/article/21cb104db5ac4bb897f5e061cc4c9fef2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80269-2https://doaj.org/toc/2045-2322Abstract Electrospun fibrous scaffolds combined with bioactive factors can display impressive performance as an ideal wound dressing, since they can mimic the composition and physicochemical properties of the extracellular matrix (ECM). The aim of this study was to fabricate a new composite biomaterial (IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffold) for wound healing, using a rat model for experimental evaluation. A small pentapeptide tag composed of DA–Lys–DA–Lys–DA residues was introduced into insulin-like growth factor 1 (IGF1) and the antimicrobial peptide Os to prepare IGF1 and Os modified with 3,4-dihydroxyphenylalanine (DA) (IGF1-DA and Os-DA). The designed chimeric growth factor and antimicrobial peptide could successfully anchor to PLGA electrospun fibrous scaffolds, and the growth factor and antimicrobial peptide could be controllably released from the electrospun fibrous scaffolds. The results showed that the IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffolds (PLGA/Os-DA/IGF1-DA) exhibited high hydrophilicity and antimicrobial activity; moreover, the porous network of the scaffolds was similar to that of the natural ECM, which can provide a favourable environment for BALB/C 3T3 cells growth. The in vivo application of PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds in rat skin wounds resulted in improved wound recovery and tissue regeneration rate. The experimental results indicated that the IGF1-DA and Os-DA could effectively bind to PLGA electrospun fibrous scaffolds, promote wound healing and prevent infection in rats, thereby suggesting that PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds have a wide application value in the field of skin wound repair.Jianhang JiaoChuangang PengChen LiZhiping QiJing ZhanSu PanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jianhang Jiao Chuangang Peng Chen Li Zhiping Qi Jing Zhan Su Pan Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
description	Abstract Electrospun fibrous scaffolds combined with bioactive factors can display impressive performance as an ideal wound dressing, since they can mimic the composition and physicochemical properties of the extracellular matrix (ECM). The aim of this study was to fabricate a new composite biomaterial (IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffold) for wound healing, using a rat model for experimental evaluation. A small pentapeptide tag composed of DA–Lys–DA–Lys–DA residues was introduced into insulin-like growth factor 1 (IGF1) and the antimicrobial peptide Os to prepare IGF1 and Os modified with 3,4-dihydroxyphenylalanine (DA) (IGF1-DA and Os-DA). The designed chimeric growth factor and antimicrobial peptide could successfully anchor to PLGA electrospun fibrous scaffolds, and the growth factor and antimicrobial peptide could be controllably released from the electrospun fibrous scaffolds. The results showed that the IGF1-DA and Os-DA-modified PLGA electrospun fibrous scaffolds (PLGA/Os-DA/IGF1-DA) exhibited high hydrophilicity and antimicrobial activity; moreover, the porous network of the scaffolds was similar to that of the natural ECM, which can provide a favourable environment for BALB/C 3T3 cells growth. The in vivo application of PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds in rat skin wounds resulted in improved wound recovery and tissue regeneration rate. The experimental results indicated that the IGF1-DA and Os-DA could effectively bind to PLGA electrospun fibrous scaffolds, promote wound healing and prevent infection in rats, thereby suggesting that PLGA/Os-DA/IGF1-DA electrospun fibrous scaffolds have a wide application value in the field of skin wound repair.
format	article
author	Jianhang Jiao Chuangang Peng Chen Li Zhiping Qi Jing Zhan Su Pan
author_facet	Jianhang Jiao Chuangang Peng Chen Li Zhiping Qi Jing Zhan Su Pan
author_sort	Jianhang Jiao
title	Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
title_short	Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
title_full	Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
title_fullStr	Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
title_full_unstemmed	Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
title_sort	dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/21cb104db5ac4bb897f5e061cc4c9fef
work_keys_str_mv	AT jianhangjiao dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics AT chuangangpeng dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics AT chenli dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics AT zhipingqi dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics AT jingzhan dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics AT supan dualbioactivefactorswithadhesionfunctionmodifiedelectrospunfibrousscaffoldforskinwoundandinfectionstherapeutics
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Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics

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