Intestinal Lymphatic Endothelial Cells Produce R-Spondin3

Abstract The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We foun...

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Autores principales:	Reiki Ogasawara, Daigo Hashimoto, Shunsuke Kimura, Eiko Hayase, Takahide Ara, Shuichiro Takahashi, Hiroyuki Ohigashi, Kosuke Yoshioka, Takahiro Tateno, Emi Yokoyama, Ko Ebata, Takeshi Kondo, Junichi Sugita, Masahiro Onozawa, Toshihiko Iwanaga, Takanori Teshima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21d1a0abb22b4c0eb8207b01cd6ddb8b
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spelling	oai:doaj.org-article:21d1a0abb22b4c0eb8207b01cd6ddb8b2021-12-02T15:08:34ZIntestinal Lymphatic Endothelial Cells Produce R-Spondin310.1038/s41598-018-29100-72045-2322https://doaj.org/article/21d1a0abb22b4c0eb8207b01cd6ddb8b2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29100-7https://doaj.org/toc/2045-2322Abstract The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45−CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.Reiki OgasawaraDaigo HashimotoShunsuke KimuraEiko HayaseTakahide AraShuichiro TakahashiHiroyuki OhigashiKosuke YoshiokaTakahiro TatenoEmi YokoyamaKo EbataTakeshi KondoJunichi SugitaMasahiro OnozawaToshihiko IwanagaTakanori TeshimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reiki Ogasawara Daigo Hashimoto Shunsuke Kimura Eiko Hayase Takahide Ara Shuichiro Takahashi Hiroyuki Ohigashi Kosuke Yoshioka Takahiro Tateno Emi Yokoyama Ko Ebata Takeshi Kondo Junichi Sugita Masahiro Onozawa Toshihiko Iwanaga Takanori Teshima Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
description	Abstract The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45−CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.
format	article
author	Reiki Ogasawara Daigo Hashimoto Shunsuke Kimura Eiko Hayase Takahide Ara Shuichiro Takahashi Hiroyuki Ohigashi Kosuke Yoshioka Takahiro Tateno Emi Yokoyama Ko Ebata Takeshi Kondo Junichi Sugita Masahiro Onozawa Toshihiko Iwanaga Takanori Teshima
author_facet	Reiki Ogasawara Daigo Hashimoto Shunsuke Kimura Eiko Hayase Takahide Ara Shuichiro Takahashi Hiroyuki Ohigashi Kosuke Yoshioka Takahiro Tateno Emi Yokoyama Ko Ebata Takeshi Kondo Junichi Sugita Masahiro Onozawa Toshihiko Iwanaga Takanori Teshima
author_sort	Reiki Ogasawara
title	Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
title_short	Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
title_full	Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
title_fullStr	Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
title_full_unstemmed	Intestinal Lymphatic Endothelial Cells Produce R-Spondin3
title_sort	intestinal lymphatic endothelial cells produce r-spondin3
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/21d1a0abb22b4c0eb8207b01cd6ddb8b
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Intestinal Lymphatic Endothelial Cells Produce R-Spondin3

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