Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis

Introduction: Herbal plants are important sources for finding new and rare products of medicinal value for drug development. The present research aimed to investigate the antibacterial properties of Dendrostellera lessertii against infectious bacteria and analyze its chemical composition. Methods: T...

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Autor principal:	Mostafa Alamholo
Formato:	article
Lenguaje:	EN
Publicado:	Zabol University of Medical sciences 2021
Materias:	dendrostellera lessertii gc/ms infectious bacteria secondary metabolites Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/21d3d58bc8d3438a87876196cf979dfc
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spelling	oai:doaj.org-article:21d3d58bc8d3438a87876196cf979dfc2021-11-17T09:55:03ZInvestigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis2476-664X10.34172/ijbsm.2021.05https://doaj.org/article/21d3d58bc8d3438a87876196cf979dfc2021-03-01T00:00:00Zhttp://ijbsm.zbmu.ac.ir/PDF/ijbsm-21470https://doaj.org/toc/2476-664XIntroduction: Herbal plants are important sources for finding new and rare products of medicinal value for drug development. The present research aimed to investigate the antibacterial properties of Dendrostellera lessertii against infectious bacteria and analyze its chemical composition. Methods: The different organs comprising root, stem, and leaf of D. lessertii from Lorestan province, Iran, were tested. Antibacterial activity was assessed using the agar well-diffusion assay. The total phenolic content (TPC) and total flavonoid content (TFC) were assessed by the Folin Ciocalteu and aluminum chloride methods, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by the serial dilution method, and chemical compositions were analyzed by gas chromatography-mass spectrometry (GC/MS). Results: The chemical composition analysis showed the dominance of bergamotol (10.62%) and bis (2-ethylhexyl) phthalate (7.49%) in the stem extract. However, phytol (12.64%) and E-11-hexadecenal (12.53%) were major constituents in the root extract. Furthermore, major constituents in the leaf extract were phytol (19.658%) and hexadecanoic acid (7.151%). The methanolic extract of root exhibited the highest TPC as 109.1±2.2 mgGA/g and TFC as 2.1±0.33 mgQ/g. The root methanolic extract demonstrated a MIC of 3.125 mg/mL against Enterococcus faecalis. Accordingly, the highest sensitivity and resistance were observed on E. faecalis and Pseudomonas aeruginosa, respectively. Conclusion: Dendrostellera lessertii extract is suggested as a source for antimicrobial drugs, especially to treat bacterial infections.Mostafa AlamholoZabol University of Medical sciencesarticledendrostellera lessertii gc/ms infectious bacteria secondary metabolites Medicine (General)R5-920ENInternational Journal of Basic Science in Medicine, Vol 6, Iss 1, Pp 30-36 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dendrostellera lessertii gc/ms infectious bacteria secondary metabolites Medicine (General) R5-920
spellingShingle	dendrostellera lessertii gc/ms infectious bacteria secondary metabolites Medicine (General) R5-920 Mostafa Alamholo Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
description	Introduction: Herbal plants are important sources for finding new and rare products of medicinal value for drug development. The present research aimed to investigate the antibacterial properties of Dendrostellera lessertii against infectious bacteria and analyze its chemical composition. Methods: The different organs comprising root, stem, and leaf of D. lessertii from Lorestan province, Iran, were tested. Antibacterial activity was assessed using the agar well-diffusion assay. The total phenolic content (TPC) and total flavonoid content (TFC) were assessed by the Folin Ciocalteu and aluminum chloride methods, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by the serial dilution method, and chemical compositions were analyzed by gas chromatography-mass spectrometry (GC/MS). Results: The chemical composition analysis showed the dominance of bergamotol (10.62%) and bis (2-ethylhexyl) phthalate (7.49%) in the stem extract. However, phytol (12.64%) and E-11-hexadecenal (12.53%) were major constituents in the root extract. Furthermore, major constituents in the leaf extract were phytol (19.658%) and hexadecanoic acid (7.151%). The methanolic extract of root exhibited the highest TPC as 109.1±2.2 mgGA/g and TFC as 2.1±0.33 mgQ/g. The root methanolic extract demonstrated a MIC of 3.125 mg/mL against Enterococcus faecalis. Accordingly, the highest sensitivity and resistance were observed on E. faecalis and Pseudomonas aeruginosa, respectively. Conclusion: Dendrostellera lessertii extract is suggested as a source for antimicrobial drugs, especially to treat bacterial infections.
format	article
author	Mostafa Alamholo
author_facet	Mostafa Alamholo
author_sort	Mostafa Alamholo
title	Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
title_short	Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
title_full	Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
title_fullStr	Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
title_full_unstemmed	Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis
title_sort	investigation of the resistance and sensitivity of infectious bacteria to dendrostellera lessertii and chemical composition analysis
publisher	Zabol University of Medical sciences
publishDate	2021
url	https://doaj.org/article/21d3d58bc8d3438a87876196cf979dfc
work_keys_str_mv	AT mostafaalamholo investigationoftheresistanceandsensitivityofinfectiousbacteriatodendrostelleralessertiiandchemicalcompositionanalysis
_version_	1718425596143337472

Investigation of the Resistance and Sensitivity of Infectious Bacteria to Dendrostellera lessertii and Chemical Composition Analysis

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