Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan

Abstract The aims of the present study were to profile the antimicrobial susceptibility patterns of a diverse range of Nocardia species isolated in Japan, and to determine the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species/complex i...

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Autores principales:	Masahiro Toyokawa, Noboru Ohana, Akiko Ueda, Minako Imai, Daiki Tanno, Mutsuko Honda, Yukiko Takano, Kazutaka Ohashi, Kyoichi Saito, Hiroki Shimura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/21f140bb4a6947ce80950276f91e0f9f
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spelling	oai:doaj.org-article:21f140bb4a6947ce80950276f91e0f9f2021-12-02T17:08:23ZIdentification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan10.1038/s41598-021-95870-22045-2322https://doaj.org/article/21f140bb4a6947ce80950276f91e0f9f2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95870-2https://doaj.org/toc/2045-2322Abstract The aims of the present study were to profile the antimicrobial susceptibility patterns of a diverse range of Nocardia species isolated in Japan, and to determine the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species/complex identification. Identification of 153 clinical isolates was performed by full-length 16S rRNA gene sequencing as a reference method to evaluate the usefulness of MALDI-TOF MS identification. Antimicrobial susceptibility testing (AST) for 14 antibiotics was performed using the broth microdilution method against 146 of the isolates. Among the total 153 clinical isolates, Nocardia farcinica complex (25%) was the most common species, followed by Nocardia cyriacigeorgica (18%), Nocardia brasiliensis (9%), Nocardia nova (8%), and Nocardia otitidiscaviarum (7%). Among 150 isolates identified to the species/complex level by 16S rRNA gene sequencing, MALDI-TOF MS with the use of a supplemental Nocardia library (JMLD library ver.ML01) correctly identified 97.3% (n = 146) to the species/complex level and 1.3% (n = 2) to the genus level. Among the 146 Nocardia isolates that underwent AST, the susceptibilities were 100% to linezolid, 96% to amikacin, 94% to trimethoprim-sulfamethoxazole, and 76% to imipenem. None of the trimethoprim-sulfamethoxazole-resistant isolates carried either plasmid-mediated sulfonamide-resistant genes (sul1, sul2) or trimethoprim-resistant genes (dfrA).Masahiro ToyokawaNoboru OhanaAkiko UedaMinako ImaiDaiki TannoMutsuko HondaYukiko TakanoKazutaka OhashiKyoichi SaitoHiroki ShimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masahiro Toyokawa Noboru Ohana Akiko Ueda Minako Imai Daiki Tanno Mutsuko Honda Yukiko Takano Kazutaka Ohashi Kyoichi Saito Hiroki Shimura Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
description	Abstract The aims of the present study were to profile the antimicrobial susceptibility patterns of a diverse range of Nocardia species isolated in Japan, and to determine the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species/complex identification. Identification of 153 clinical isolates was performed by full-length 16S rRNA gene sequencing as a reference method to evaluate the usefulness of MALDI-TOF MS identification. Antimicrobial susceptibility testing (AST) for 14 antibiotics was performed using the broth microdilution method against 146 of the isolates. Among the total 153 clinical isolates, Nocardia farcinica complex (25%) was the most common species, followed by Nocardia cyriacigeorgica (18%), Nocardia brasiliensis (9%), Nocardia nova (8%), and Nocardia otitidiscaviarum (7%). Among 150 isolates identified to the species/complex level by 16S rRNA gene sequencing, MALDI-TOF MS with the use of a supplemental Nocardia library (JMLD library ver.ML01) correctly identified 97.3% (n = 146) to the species/complex level and 1.3% (n = 2) to the genus level. Among the 146 Nocardia isolates that underwent AST, the susceptibilities were 100% to linezolid, 96% to amikacin, 94% to trimethoprim-sulfamethoxazole, and 76% to imipenem. None of the trimethoprim-sulfamethoxazole-resistant isolates carried either plasmid-mediated sulfonamide-resistant genes (sul1, sul2) or trimethoprim-resistant genes (dfrA).
format	article
author	Masahiro Toyokawa Noboru Ohana Akiko Ueda Minako Imai Daiki Tanno Mutsuko Honda Yukiko Takano Kazutaka Ohashi Kyoichi Saito Hiroki Shimura
author_facet	Masahiro Toyokawa Noboru Ohana Akiko Ueda Minako Imai Daiki Tanno Mutsuko Honda Yukiko Takano Kazutaka Ohashi Kyoichi Saito Hiroki Shimura
author_sort	Masahiro Toyokawa
title	Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
title_short	Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
title_full	Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
title_fullStr	Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
title_full_unstemmed	Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan
title_sort	identification and antimicrobial susceptibility profiles of nocardia species clinically isolated in japan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/21f140bb4a6947ce80950276f91e0f9f
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_version_	1718381542237011968

Identification and antimicrobial susceptibility profiles of Nocardia species clinically isolated in Japan

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