K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat

Juan Sun, Jian Wang, Luoman Hu, Jinfeng Yan Rehabilitation Department, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province 266000, People’s Republic of ChinaCorrespondence: Juan SunRehabilitation Department, The Affiliated Hospital of Qingdao University, No. 1677...

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Autores principales:	Sun J, Wang J, Hu L, Yan J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	kaempferol-3-o-rhamnoside pi3k/akt apoptosis cerebral ischemia/reperfusion (i/r) injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/2204b143d2b84547933cac7dd4168f04
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spelling	oai:doaj.org-article:2204b143d2b84547933cac7dd4168f042021-12-02T12:00:35ZK-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat1178-2021https://doaj.org/article/2204b143d2b84547933cac7dd4168f042020-05-01T00:00:00Zhttps://www.dovepress.com/k-3-rh-protects-against-cerebral-ischemiareperfusion-injury-by-anti-ap-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Juan Sun, Jian Wang, Luoman Hu, Jinfeng Yan Rehabilitation Department, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province 266000, People’s Republic of ChinaCorrespondence: Juan SunRehabilitation Department, The Affiliated Hospital of Qingdao University, No. 1677 Wutaishan Road, Qingdao City, Shandong Province 266000, People’s Republic of ChinaEmail yg4170@163.comBackground/Aims: Ischemic stroke is the main cause of nerve damage and brain dysfunction, accompanied by strong brain cell apoptosis. This study aimed to investigate the effect of kaempferol-3-O-rhamnoside (K-3-rh) on cerebral ischemia-reperfusion (I/R) injury.Methods and Materials: A rat model of cerebral I/R injury was established. The effects of K-3-rh on cerebral infarction size, brain water content and neurological deficits in rats were evaluated. Apoptosis of ischemic brain cells after mouse I/R was observed by TUNEL staining and flow cytometry. Western blot and qRT-PCR were used to detect the effect of K-3-rh on the expression of apoptosis-related proteins.Results: K-3-rh can improve the neurological deficit score, reduce the infarct volume and brain water content, and inhibit cell apoptosis. In addition, K-3-rh significantly downregulated the expression of Bax and p53 and upregulated the expression of Bcl-2, and the phosphorylation level of Akt. Blockade of PI3K activity by the PI3K inhibitor wortmannin not only reversed the effects of K-3-rh on infarct volume and brain water content but also reversed the expression level of p-Akt.Conclusion: K-3-rh had obvious neuroprotective effects on brain I/R injury and neuronal apoptosis, and its mechanism may be related to activation of PI3K/Akt signaling pathway.Keywords: kaempferol-3-O-rhamnoside, PI3K; Akt, apoptosis, cerebral ischemia/reperfusion injurySun JWang JHu LYan JDove Medical Pressarticlekaempferol-3-o-rhamnosidepi3k/aktapoptosiscerebral ischemia/reperfusion (i/r) injuryNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 1217-1227 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	kaempferol-3-o-rhamnoside pi3k/akt apoptosis cerebral ischemia/reperfusion (i/r) injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	kaempferol-3-o-rhamnoside pi3k/akt apoptosis cerebral ischemia/reperfusion (i/r) injury Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Sun J Wang J Hu L Yan J K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
description	Juan Sun, Jian Wang, Luoman Hu, Jinfeng Yan Rehabilitation Department, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province 266000, People’s Republic of ChinaCorrespondence: Juan SunRehabilitation Department, The Affiliated Hospital of Qingdao University, No. 1677 Wutaishan Road, Qingdao City, Shandong Province 266000, People’s Republic of ChinaEmail yg4170@163.comBackground/Aims: Ischemic stroke is the main cause of nerve damage and brain dysfunction, accompanied by strong brain cell apoptosis. This study aimed to investigate the effect of kaempferol-3-O-rhamnoside (K-3-rh) on cerebral ischemia-reperfusion (I/R) injury.Methods and Materials: A rat model of cerebral I/R injury was established. The effects of K-3-rh on cerebral infarction size, brain water content and neurological deficits in rats were evaluated. Apoptosis of ischemic brain cells after mouse I/R was observed by TUNEL staining and flow cytometry. Western blot and qRT-PCR were used to detect the effect of K-3-rh on the expression of apoptosis-related proteins.Results: K-3-rh can improve the neurological deficit score, reduce the infarct volume and brain water content, and inhibit cell apoptosis. In addition, K-3-rh significantly downregulated the expression of Bax and p53 and upregulated the expression of Bcl-2, and the phosphorylation level of Akt. Blockade of PI3K activity by the PI3K inhibitor wortmannin not only reversed the effects of K-3-rh on infarct volume and brain water content but also reversed the expression level of p-Akt.Conclusion: K-3-rh had obvious neuroprotective effects on brain I/R injury and neuronal apoptosis, and its mechanism may be related to activation of PI3K/Akt signaling pathway.Keywords: kaempferol-3-O-rhamnoside, PI3K; Akt, apoptosis, cerebral ischemia/reperfusion injury
format	article
author	Sun J Wang J Hu L Yan J
author_facet	Sun J Wang J Hu L Yan J
author_sort	Sun J
title	K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
title_short	K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
title_full	K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
title_fullStr	K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
title_full_unstemmed	K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat
title_sort	k-3-rh protects against cerebral ischemia/reperfusion injury by anti-apoptotic effect through pi3k-akt signaling pathway in rat
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/2204b143d2b84547933cac7dd4168f04
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K-3-Rh Protects Against Cerebral Ischemia/Reperfusion Injury by Anti-Apoptotic Effect Through PI3K-Akt Signaling Pathway in Rat

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