Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)

The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of...

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Autores principales:	Laura Robles-Gómez, Paula Sáez-Espinosa, Eliana Marina López-Viloria, Andrea López-Botella, Jon Aizpurua, María José Gómez-Torres
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aleuria aurantia</i> lectin capacitation FE-SEM fucose glycoconjugates sperm Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2205fd936f90466993c41b7bdfacc835
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spelling	oai:doaj.org-article:2205fd936f90466993c41b7bdfacc8352021-11-11T17:21:47ZQuantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)10.3390/ijms2221119471422-00671661-6596https://doaj.org/article/2205fd936f90466993c41b7bdfacc8352021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11947https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of terminal and linked fucose is still unknown. In this study, the quantitative and qualitative changes in fucosyl residues during different in vitro capacitation times (1 and 4 h), are simultaneously characterized by using <i>Aleuria aurantia</i> (AAA) lectin–gold labelling and high-resolution field emission scanning electron microscopy (FE-SEM) in human sperm. A significant decrease was found in the number of terminal fucose registered in the whole sperm head during the in vitro capacitation. Nevertheless, the quantification of fucose residues after 1 h of in vitro capacitation was very similar to those found after 4 h. Therefore, the changes observed in terminal and linked fucose during capacitation were not time-dependent. Furthermore, the comprehensive analysis of the topographic distribution showed the preferential fucosyl location in the acrosomal region and the presence of distinct clusters distributed over the head in all the studied conditions. Overall, these findings corroborate the validity of FE-SEM combined with gold labelling to register changes in surface molecules during in vitro sperm capacitation.Laura Robles-GómezPaula Sáez-EspinosaEliana Marina López-ViloriaAndrea López-BotellaJon AizpuruaMaría José Gómez-TorresMDPI AGarticle<i>Aleuria aurantia</i> lectincapacitationFE-SEMfucoseglycoconjugatesspermBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11947, p 11947 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aleuria aurantia</i> lectin capacitation FE-SEM fucose glycoconjugates sperm Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Aleuria aurantia</i> lectin capacitation FE-SEM fucose glycoconjugates sperm Biology (General) QH301-705.5 Chemistry QD1-999 Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
description	The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of terminal and linked fucose is still unknown. In this study, the quantitative and qualitative changes in fucosyl residues during different in vitro capacitation times (1 and 4 h), are simultaneously characterized by using <i>Aleuria aurantia</i> (AAA) lectin–gold labelling and high-resolution field emission scanning electron microscopy (FE-SEM) in human sperm. A significant decrease was found in the number of terminal fucose registered in the whole sperm head during the in vitro capacitation. Nevertheless, the quantification of fucose residues after 1 h of in vitro capacitation was very similar to those found after 4 h. Therefore, the changes observed in terminal and linked fucose during capacitation were not time-dependent. Furthermore, the comprehensive analysis of the topographic distribution showed the preferential fucosyl location in the acrosomal region and the presence of distinct clusters distributed over the head in all the studied conditions. Overall, these findings corroborate the validity of FE-SEM combined with gold labelling to register changes in surface molecules during in vitro sperm capacitation.
format	article
author	Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres
author_facet	Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres
author_sort	Laura Robles-Gómez
title	Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
title_short	Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
title_full	Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
title_fullStr	Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
title_full_unstemmed	Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
title_sort	quantification and topographical distribution of terminal and linked fucose residues in human spermatozoa by using field emission scanning electron microscopy (fe-sem)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2205fd936f90466993c41b7bdfacc835
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Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)

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