Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy

Pei-Chi Lee,1 Bo-Shen Zan,1 Li-Ting Chen,1 Tze-Wen Chung1,2 1Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan; 2Drug Delivery Department, Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang Ming University, Taipei 112, Taiwan Backgroun...

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Autores principales:	Lee PC, Zan BS, Chen LT, Chung TW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ischemia/reperfusion (I/R) heparin glutathione anticoagulant antioxidant human bone marrow mesenchymal stem cells (hBMSCs) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2207cf04ddaa495eaefd28aee9a1aac1
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spelling	oai:doaj.org-article:2207cf04ddaa495eaefd28aee9a1aac12021-12-02T03:58:49ZMultifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy1178-2013https://doaj.org/article/2207cf04ddaa495eaefd28aee9a1aac12019-02-01T00:00:00Zhttps://www.dovepress.com/multifunctional-plga-based-nanoparticles-as-a-controlled-release-drug--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pei-Chi Lee,1 Bo-Shen Zan,1 Li-Ting Chen,1 Tze-Wen Chung1,2 1Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan; 2Drug Delivery Department, Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang Ming University, Taipei 112, Taiwan Background: Ischemia/reperfusion (I/R) injury causes the generation of many ROS such as H2O2 and leads to vascular thrombosis, which causes tissue damage. Purpose: In this investigation, poly (lactideco-glycolide) (PLGA)-based nanoparticles are used for their anticoagulant and antioxidant properties in vascular therapy. Methods: Both heparin and glutathione are entrapped on PLGA-stearylamine nanoparticles by layer-by-layer interactions. Results: The drug release rate is successfully controlled with only 10.3% of the heparin released after 96 hours. An H2O2-responsive platform is also developed by combining silk fibroin and horse peroxidase to detect H2O2 in this drug delivery system. Besides, hyaluronic acid was decorated on the surface of nanoparticles to target the human bone marrow mesenchymal stem cells (hBMSCs) for cell therapy. The results of an in vitro study indicate that the nanoparticles could be taken up by hBMSCs within 2 hours and exocytosis occurred 6 hours after cellular uptake. Conclusion: We propose that the multifunctional nanoparticles that are formed herein can be effectively delivered to the site of an I/R injury via the hBMSC homing effect. The proposed approach can potentially be used to treat vascular diseases, providing a platform for hBMSCs for the controlled delivery of a wide range of drugs. Keywords: ischemia/reperfusion, I/R, heparin, glutathione, anticoagulant, antioxidant, human bone marrow mesenchymal stem cells, hBMSCsLee PCZan BSChen LTChung TWDove Medical Pressarticleischemia/reperfusion (I/R)heparinglutathioneanticoagulantantioxidanthuman bone marrow mesenchymal stem cells (hBMSCs)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1533-1549 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ischemia/reperfusion (I/R) heparin glutathione anticoagulant antioxidant human bone marrow mesenchymal stem cells (hBMSCs) Medicine (General) R5-920
spellingShingle	ischemia/reperfusion (I/R) heparin glutathione anticoagulant antioxidant human bone marrow mesenchymal stem cells (hBMSCs) Medicine (General) R5-920 Lee PC Zan BS Chen LT Chung TW Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
description	Pei-Chi Lee,1 Bo-Shen Zan,1 Li-Ting Chen,1 Tze-Wen Chung1,2 1Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan; 2Drug Delivery Department, Center for Advanced Pharmaceutics and Drug Delivery Research, National Yang Ming University, Taipei 112, Taiwan Background: Ischemia/reperfusion (I/R) injury causes the generation of many ROS such as H2O2 and leads to vascular thrombosis, which causes tissue damage. Purpose: In this investigation, poly (lactideco-glycolide) (PLGA)-based nanoparticles are used for their anticoagulant and antioxidant properties in vascular therapy. Methods: Both heparin and glutathione are entrapped on PLGA-stearylamine nanoparticles by layer-by-layer interactions. Results: The drug release rate is successfully controlled with only 10.3% of the heparin released after 96 hours. An H2O2-responsive platform is also developed by combining silk fibroin and horse peroxidase to detect H2O2 in this drug delivery system. Besides, hyaluronic acid was decorated on the surface of nanoparticles to target the human bone marrow mesenchymal stem cells (hBMSCs) for cell therapy. The results of an in vitro study indicate that the nanoparticles could be taken up by hBMSCs within 2 hours and exocytosis occurred 6 hours after cellular uptake. Conclusion: We propose that the multifunctional nanoparticles that are formed herein can be effectively delivered to the site of an I/R injury via the hBMSC homing effect. The proposed approach can potentially be used to treat vascular diseases, providing a platform for hBMSCs for the controlled delivery of a wide range of drugs. Keywords: ischemia/reperfusion, I/R, heparin, glutathione, anticoagulant, antioxidant, human bone marrow mesenchymal stem cells, hBMSCs
format	article
author	Lee PC Zan BS Chen LT Chung TW
author_facet	Lee PC Zan BS Chen LT Chung TW
author_sort	Lee PC
title	Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
title_short	Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
title_full	Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
title_fullStr	Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
title_full_unstemmed	Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
title_sort	multifunctional plga-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/2207cf04ddaa495eaefd28aee9a1aac1
work_keys_str_mv	AT leepc multifunctionalplgabasednanoparticlesasacontrolledreleasedrugdeliverysystemforantioxidantandanticoagulanttherapy AT zanbs multifunctionalplgabasednanoparticlesasacontrolledreleasedrugdeliverysystemforantioxidantandanticoagulanttherapy AT chenlt multifunctionalplgabasednanoparticlesasacontrolledreleasedrugdeliverysystemforantioxidantandanticoagulanttherapy AT chungtw multifunctionalplgabasednanoparticlesasacontrolledreleasedrugdeliverysystemforantioxidantandanticoagulanttherapy
_version_	1718401476539187200

Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy

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