Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan

Al-Zarqa River is the second main tributary to River Jordan after the Yarmouk River. The river flow has been modified by discharge of industrial wastewater and treated domestic water. Concerns about the occurrence of waterborne pathogenic viruses in the surface waters of Al-Zarqa River prompted the...

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Autores principales:	Ismail Saadoun, Qutaiba Ababneh, Ziad Jaradat
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	adenoviruses astroviruses enteric hepatitis a viruses wastewater zarqa river Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/2210389e708c40adbcab943503cc8309
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spelling	oai:doaj.org-article:2210389e708c40adbcab943503cc83092021-11-06T06:28:23ZGenomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan1477-89201996-782910.2166/wh.2021.038https://doaj.org/article/2210389e708c40adbcab943503cc83092021-08-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/4/604https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Al-Zarqa River is the second main tributary to River Jordan after the Yarmouk River. The river flow has been modified by discharge of industrial wastewater and treated domestic water. Concerns about the occurrence of waterborne pathogenic viruses in the surface waters of Al-Zarqa River prompted the analysis of the surface water quality with respect to the presence of enteric viruses. Viruses were concentrated from a total of 33 different water environmental samples including raw sewage, effluent samples and river water collected from and around the river over a period of 11 months. Calculated recovery yields for these concentration methods ranged between 2 and 8%. Polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR), nested RT-PCR and southern blotting hybridization analysis were used for the detection of hepatitis A virus, norovirus, astrovirus and human adenovirus 40/41, with the later one being detected in 21 (64%) of the samples that also showed previous positive presence for enteroviruses. To our knowledge, this is the first molecular biology report in Jordan describing the circulation of adenoviruses, which were detected more frequently than enteroviruses in sewage and water samples, and therefore, they can be used as an index for the presence of human pathogenic viruses in water environment. HIGHLIGHTS Occurrence of waterborne pathogenic viruses in water resources.; Circulation of adenoviruses from and around Al-Zarqa River, Jordan.; Implementation of techniques for concentrating viruses from surface and treated water, and raw sewage.; Genomic content of enteric viruses was detected by molecular techniques.; Adenoviruses can be used as an index for the presence of human pathogenic viruses in water environment.;Ismail SaadounQutaiba AbabnehZiad JaradatIWA Publishingarticleadenovirusesastrovirusesenterichepatitis a viruseswastewaterzarqa riverPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 4, Pp 604-615 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenoviruses astroviruses enteric hepatitis a viruses wastewater zarqa river Public aspects of medicine RA1-1270
spellingShingle	adenoviruses astroviruses enteric hepatitis a viruses wastewater zarqa river Public aspects of medicine RA1-1270 Ismail Saadoun Qutaiba Ababneh Ziad Jaradat Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
description	Al-Zarqa River is the second main tributary to River Jordan after the Yarmouk River. The river flow has been modified by discharge of industrial wastewater and treated domestic water. Concerns about the occurrence of waterborne pathogenic viruses in the surface waters of Al-Zarqa River prompted the analysis of the surface water quality with respect to the presence of enteric viruses. Viruses were concentrated from a total of 33 different water environmental samples including raw sewage, effluent samples and river water collected from and around the river over a period of 11 months. Calculated recovery yields for these concentration methods ranged between 2 and 8%. Polymerase chain reaction (PCR), reverse transcriptase-PCR (RT-PCR), nested RT-PCR and southern blotting hybridization analysis were used for the detection of hepatitis A virus, norovirus, astrovirus and human adenovirus 40/41, with the later one being detected in 21 (64%) of the samples that also showed previous positive presence for enteroviruses. To our knowledge, this is the first molecular biology report in Jordan describing the circulation of adenoviruses, which were detected more frequently than enteroviruses in sewage and water samples, and therefore, they can be used as an index for the presence of human pathogenic viruses in water environment. HIGHLIGHTS Occurrence of waterborne pathogenic viruses in water resources.; Circulation of adenoviruses from and around Al-Zarqa River, Jordan.; Implementation of techniques for concentrating viruses from surface and treated water, and raw sewage.; Genomic content of enteric viruses was detected by molecular techniques.; Adenoviruses can be used as an index for the presence of human pathogenic viruses in water environment.;
format	article
author	Ismail Saadoun Qutaiba Ababneh Ziad Jaradat
author_facet	Ismail Saadoun Qutaiba Ababneh Ziad Jaradat
author_sort	Ismail Saadoun
title	Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
title_short	Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
title_full	Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
title_fullStr	Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
title_full_unstemmed	Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan
title_sort	genomic detection of waterborne enteric viruses as water quality indicators in al-zarqa river, jordan
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/2210389e708c40adbcab943503cc8309
work_keys_str_mv	AT ismailsaadoun genomicdetectionofwaterborneentericvirusesaswaterqualityindicatorsinalzarqariverjordan AT qutaibaababneh genomicdetectionofwaterborneentericvirusesaswaterqualityindicatorsinalzarqariverjordan AT ziadjaradat genomicdetectionofwaterborneentericvirusesaswaterqualityindicatorsinalzarqariverjordan
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Genomic detection of waterborne enteric viruses as water quality indicators in Al-Zarqa River, Jordan

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