Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells

Abstract Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements – it is therefore important to determine...

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Autores principales:	Eduardo Fernandez-Rebollo, Birgit Mentrup, Regina Ebert, Julia Franzen, Giulio Abagnale, Torsten Sieben, Alina Ostrowska, Per Hoffmann, Pierre-François Roux, Björn Rath, Michele Goodhardt, Jean-Marc Lemaitre, Oliver Bischof, Franz Jakob, Wolfgang Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2212fb9151c240eb8164a2503a67027e
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spelling	oai:doaj.org-article:2212fb9151c240eb8164a2503a67027e2021-12-02T11:52:44ZHuman Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells10.1038/s41598-017-05207-12045-2322https://doaj.org/article/2212fb9151c240eb8164a2503a67027e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05207-1https://doaj.org/toc/2045-2322Abstract Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements – it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology. Remarkably, global DNA-methylation profiles did not reveal any significant differences. Even at the transcriptomic level, there were only moderate changes in pairwise comparison. Furthermore, the effects on proliferation, cytoskeletal organization, and focal adhesions were reversible by interchanging to opposite culture conditions. These results indicate that cultivation of MSCs with HPL or FCS has no systematic bias for specific cell types.Eduardo Fernandez-RebolloBirgit MentrupRegina EbertJulia FranzenGiulio AbagnaleTorsten SiebenAlina OstrowskaPer HoffmannPierre-François RouxBjörn RathMichele GoodhardtJean-Marc LemaitreOliver BischofFranz JakobWolfgang WagnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eduardo Fernandez-Rebollo Birgit Mentrup Regina Ebert Julia Franzen Giulio Abagnale Torsten Sieben Alina Ostrowska Per Hoffmann Pierre-François Roux Björn Rath Michele Goodhardt Jean-Marc Lemaitre Oliver Bischof Franz Jakob Wolfgang Wagner Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
description	Abstract Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements – it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology. Remarkably, global DNA-methylation profiles did not reveal any significant differences. Even at the transcriptomic level, there were only moderate changes in pairwise comparison. Furthermore, the effects on proliferation, cytoskeletal organization, and focal adhesions were reversible by interchanging to opposite culture conditions. These results indicate that cultivation of MSCs with HPL or FCS has no systematic bias for specific cell types.
format	article
author	Eduardo Fernandez-Rebollo Birgit Mentrup Regina Ebert Julia Franzen Giulio Abagnale Torsten Sieben Alina Ostrowska Per Hoffmann Pierre-François Roux Björn Rath Michele Goodhardt Jean-Marc Lemaitre Oliver Bischof Franz Jakob Wolfgang Wagner
author_facet	Eduardo Fernandez-Rebollo Birgit Mentrup Regina Ebert Julia Franzen Giulio Abagnale Torsten Sieben Alina Ostrowska Per Hoffmann Pierre-François Roux Björn Rath Michele Goodhardt Jean-Marc Lemaitre Oliver Bischof Franz Jakob Wolfgang Wagner
author_sort	Eduardo Fernandez-Rebollo
title	Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
title_short	Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
title_full	Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
title_fullStr	Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
title_full_unstemmed	Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells
title_sort	human platelet lysate versus fetal calf serum: these supplements do not select for different mesenchymal stromal cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2212fb9151c240eb8164a2503a67027e
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Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells

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