Physiological concentrations of leptin do not affect human neutrophils.

Leptin is an adipokine that is thought to be important in many inflammatory diseases, and is known to influence the function of several leukocyte types. However, no clear consensus is present regarding the responsiveness of neutrophils for this adipokine. In this study a 2D DIGE proteomics approach...

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Autores principales:	Vera M Kamp, Jeroen D Langereis, Corneli W van Aalst, Jan A van der Linden, Laurien H Ulfman, Leo Koenderman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/221bf74c049f4ebf859a0ed002019417
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spelling	oai:doaj.org-article:221bf74c049f4ebf859a0ed0020194172021-11-18T08:55:14ZPhysiological concentrations of leptin do not affect human neutrophils.1932-620310.1371/journal.pone.0073170https://doaj.org/article/221bf74c049f4ebf859a0ed0020194172013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24066032/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Leptin is an adipokine that is thought to be important in many inflammatory diseases, and is known to influence the function of several leukocyte types. However, no clear consensus is present regarding the responsiveness of neutrophils for this adipokine. In this study a 2D DIGE proteomics approach was used as an unbiased approach to identify leptin-induced effects on neutrophils. Additionally chemotaxis and survival experiments were performed to reproduce results from literature showing putative effects of leptin on these neutrophil responses. Leptin did not induce any significant changes in the proteome provided leptin was added at physiologically relevant concentrations (250 ng). Our leptin batches were biologically active as they induced proliferation in LeptinR expressing Ba/F3 cells. At high concentrations (25000 ng) leptin induced a change in neutrophil proteome. Seventeen differently regulated spots were identified of which twelve could be characterized by mass spectrometry. Two of these identified proteins, SerpinB1 and p40 phox, were chosen for further analysis but leptin-induced expression analyzed by western blot were highly variable. Additionally leptin also induced neutrophil survival at these high concentrations. No leptin-induced chemotaxis of human neutrophils was detected at any concentration. In conclusion, physiological concentrations of leptin do not affect neutrophils. High leptin concentrations induced survival and changes in the neutrophils proteome, but this was most likely mediated by an indirect effect. However, it cannot be ruled out that the effects were mediated by a yet not-identified leptin receptor on human neutrophils.Vera M KampJeroen D LangereisCorneli W van AalstJan A van der LindenLaurien H UlfmanLeo KoendermanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73170 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vera M Kamp Jeroen D Langereis Corneli W van Aalst Jan A van der Linden Laurien H Ulfman Leo Koenderman Physiological concentrations of leptin do not affect human neutrophils.
description	Leptin is an adipokine that is thought to be important in many inflammatory diseases, and is known to influence the function of several leukocyte types. However, no clear consensus is present regarding the responsiveness of neutrophils for this adipokine. In this study a 2D DIGE proteomics approach was used as an unbiased approach to identify leptin-induced effects on neutrophils. Additionally chemotaxis and survival experiments were performed to reproduce results from literature showing putative effects of leptin on these neutrophil responses. Leptin did not induce any significant changes in the proteome provided leptin was added at physiologically relevant concentrations (250 ng). Our leptin batches were biologically active as they induced proliferation in LeptinR expressing Ba/F3 cells. At high concentrations (25000 ng) leptin induced a change in neutrophil proteome. Seventeen differently regulated spots were identified of which twelve could be characterized by mass spectrometry. Two of these identified proteins, SerpinB1 and p40 phox, were chosen for further analysis but leptin-induced expression analyzed by western blot were highly variable. Additionally leptin also induced neutrophil survival at these high concentrations. No leptin-induced chemotaxis of human neutrophils was detected at any concentration. In conclusion, physiological concentrations of leptin do not affect neutrophils. High leptin concentrations induced survival and changes in the neutrophils proteome, but this was most likely mediated by an indirect effect. However, it cannot be ruled out that the effects were mediated by a yet not-identified leptin receptor on human neutrophils.
format	article
author	Vera M Kamp Jeroen D Langereis Corneli W van Aalst Jan A van der Linden Laurien H Ulfman Leo Koenderman
author_facet	Vera M Kamp Jeroen D Langereis Corneli W van Aalst Jan A van der Linden Laurien H Ulfman Leo Koenderman
author_sort	Vera M Kamp
title	Physiological concentrations of leptin do not affect human neutrophils.
title_short	Physiological concentrations of leptin do not affect human neutrophils.
title_full	Physiological concentrations of leptin do not affect human neutrophils.
title_fullStr	Physiological concentrations of leptin do not affect human neutrophils.
title_full_unstemmed	Physiological concentrations of leptin do not affect human neutrophils.
title_sort	physiological concentrations of leptin do not affect human neutrophils.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/221bf74c049f4ebf859a0ed002019417
work_keys_str_mv	AT veramkamp physiologicalconcentrationsofleptindonotaffecthumanneutrophils AT jeroendlangereis physiologicalconcentrationsofleptindonotaffecthumanneutrophils AT corneliwvanaalst physiologicalconcentrationsofleptindonotaffecthumanneutrophils AT janavanderlinden physiologicalconcentrationsofleptindonotaffecthumanneutrophils AT laurienhulfman physiologicalconcentrationsofleptindonotaffecthumanneutrophils AT leokoenderman physiologicalconcentrationsofleptindonotaffecthumanneutrophils
_version_	1718421129398321152

Physiological concentrations of leptin do not affect human neutrophils.

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