Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle

Abstract Nucleoid-associated proteins (NAPs) are responsible for maintaining highly organized and yet dynamic chromosome structure in bacteria. The genus Mycobacterium possesses a unique set of NAPs, including Lsr2, which is a DNA-bridging protein. Importantly, Lsr2 is essential for the M. tuberculo...

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Autores principales:	Marta Kołodziej, Damian Trojanowski, Katarzyna Bury, Joanna Hołówka, Weronika Matysik, Hanna Kąkolewska, Helge Feddersen, Giacomo Giacomelli, Igor Konieczny, Marc Bramkamp, Jolanta Zakrzewska-Czerwińska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/221e901fe109450a8773c0f30fbf5208
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spelling	oai:doaj.org-article:221e901fe109450a8773c0f30fbf52082021-12-02T10:44:08ZLsr2, a nucleoid-associated protein influencing mycobacterial cell cycle10.1038/s41598-021-82295-02045-2322https://doaj.org/article/221e901fe109450a8773c0f30fbf52082021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82295-0https://doaj.org/toc/2045-2322Abstract Nucleoid-associated proteins (NAPs) are responsible for maintaining highly organized and yet dynamic chromosome structure in bacteria. The genus Mycobacterium possesses a unique set of NAPs, including Lsr2, which is a DNA-bridging protein. Importantly, Lsr2 is essential for the M. tuberculosis during infection exhibiting pleiotropic activities including regulation of gene expression (mainly as a repressor). Here, we report that deletion of lsr2 gene profoundly impacts the cell morphology of M. smegmatis, which is a model organism for studying the cell biology of M. tuberculosis and other mycobacterial pathogens. Cells lacking Lsr2 are shorter, wider, and more rigid than the wild-type cells. Using time-lapse fluorescent microscopy, we showed that fluorescently tagged Lsr2 forms large and dynamic nucleoprotein complexes, and that the N-terminal oligomerization domain of Lsr2 is indispensable for the formation of nucleoprotein complexes in vivo. Moreover, lsr2 deletion exerts a significant effect on the replication time and replisome dynamics. Thus, we propose that the Lsr2 nucleoprotein complexes may contribute to maintaining the proper organization of the newly synthesized DNA and therefore influencing mycobacterial cell cycle.Marta KołodziejDamian TrojanowskiKatarzyna BuryJoanna HołówkaWeronika MatysikHanna KąkolewskaHelge FeddersenGiacomo GiacomelliIgor KoniecznyMarc BramkampJolanta Zakrzewska-CzerwińskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Kołodziej Damian Trojanowski Katarzyna Bury Joanna Hołówka Weronika Matysik Hanna Kąkolewska Helge Feddersen Giacomo Giacomelli Igor Konieczny Marc Bramkamp Jolanta Zakrzewska-Czerwińska Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
description	Abstract Nucleoid-associated proteins (NAPs) are responsible for maintaining highly organized and yet dynamic chromosome structure in bacteria. The genus Mycobacterium possesses a unique set of NAPs, including Lsr2, which is a DNA-bridging protein. Importantly, Lsr2 is essential for the M. tuberculosis during infection exhibiting pleiotropic activities including regulation of gene expression (mainly as a repressor). Here, we report that deletion of lsr2 gene profoundly impacts the cell morphology of M. smegmatis, which is a model organism for studying the cell biology of M. tuberculosis and other mycobacterial pathogens. Cells lacking Lsr2 are shorter, wider, and more rigid than the wild-type cells. Using time-lapse fluorescent microscopy, we showed that fluorescently tagged Lsr2 forms large and dynamic nucleoprotein complexes, and that the N-terminal oligomerization domain of Lsr2 is indispensable for the formation of nucleoprotein complexes in vivo. Moreover, lsr2 deletion exerts a significant effect on the replication time and replisome dynamics. Thus, we propose that the Lsr2 nucleoprotein complexes may contribute to maintaining the proper organization of the newly synthesized DNA and therefore influencing mycobacterial cell cycle.
format	article
author	Marta Kołodziej Damian Trojanowski Katarzyna Bury Joanna Hołówka Weronika Matysik Hanna Kąkolewska Helge Feddersen Giacomo Giacomelli Igor Konieczny Marc Bramkamp Jolanta Zakrzewska-Czerwińska
author_facet	Marta Kołodziej Damian Trojanowski Katarzyna Bury Joanna Hołówka Weronika Matysik Hanna Kąkolewska Helge Feddersen Giacomo Giacomelli Igor Konieczny Marc Bramkamp Jolanta Zakrzewska-Czerwińska
author_sort	Marta Kołodziej
title	Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
title_short	Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
title_full	Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
title_fullStr	Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
title_full_unstemmed	Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
title_sort	lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/221e901fe109450a8773c0f30fbf5208
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Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle

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