Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.

Antisense RNAs that originate from the complementary strand of protein coding genes are involved in the regulation of gene expression in all domains of life. In bacteria, some of these antisense RNAs are transcriptional noise while others play a vital role to adapt the cell to changing environmental...

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Autores principales:	Isaac Dadzie, Shungao Xu, Bin Ni, Xiaolei Zhang, Haifang Zhang, Xiumei Sheng, Huaxi Xu, Xinxiang Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2225266534ef48ff8c7f326e494d0844
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spelling	oai:doaj.org-article:2225266534ef48ff8c7f326e494d08442021-11-18T07:48:09ZIdentification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.1932-620310.1371/journal.pone.0061308https://doaj.org/article/2225266534ef48ff8c7f326e494d08442013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637809/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Antisense RNAs that originate from the complementary strand of protein coding genes are involved in the regulation of gene expression in all domains of life. In bacteria, some of these antisense RNAs are transcriptional noise while others play a vital role to adapt the cell to changing environmental conditions. By deep sequencing analysis of transcriptome of Salmonella enterica serovar Typhi, a partial RNA sequence encoded in-cis to the dnaA gene was revealed. Northern blot and RACE analysis confirmed the transcription of this antisense RNA which was expressed mostly in the stationary phase of the bacterial growth and also under iron limitation and osmotic stress. Pulse expression analysis showed that overexpression of the antisense RNA resulted in a significant increase in the mRNA levels of dnaA, which will ultimately enhance their translation. Our findings have revealed that antisense RNA of dnaA is indeed transcribed not merely as a by-product of the cell's transcription machinery but plays a vital role as far as stability of dnaA mRNA is concerned.Isaac DadzieShungao XuBin NiXiaolei ZhangHaifang ZhangXiumei ShengHuaxi XuXinxiang HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61308 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isaac Dadzie Shungao Xu Bin Ni Xiaolei Zhang Haifang Zhang Xiumei Sheng Huaxi Xu Xinxiang Huang Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
description	Antisense RNAs that originate from the complementary strand of protein coding genes are involved in the regulation of gene expression in all domains of life. In bacteria, some of these antisense RNAs are transcriptional noise while others play a vital role to adapt the cell to changing environmental conditions. By deep sequencing analysis of transcriptome of Salmonella enterica serovar Typhi, a partial RNA sequence encoded in-cis to the dnaA gene was revealed. Northern blot and RACE analysis confirmed the transcription of this antisense RNA which was expressed mostly in the stationary phase of the bacterial growth and also under iron limitation and osmotic stress. Pulse expression analysis showed that overexpression of the antisense RNA resulted in a significant increase in the mRNA levels of dnaA, which will ultimately enhance their translation. Our findings have revealed that antisense RNA of dnaA is indeed transcribed not merely as a by-product of the cell's transcription machinery but plays a vital role as far as stability of dnaA mRNA is concerned.
format	article
author	Isaac Dadzie Shungao Xu Bin Ni Xiaolei Zhang Haifang Zhang Xiumei Sheng Huaxi Xu Xinxiang Huang
author_facet	Isaac Dadzie Shungao Xu Bin Ni Xiaolei Zhang Haifang Zhang Xiumei Sheng Huaxi Xu Xinxiang Huang
author_sort	Isaac Dadzie
title	Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
title_short	Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
title_full	Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
title_fullStr	Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
title_full_unstemmed	Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
title_sort	identification and characterization of a cis-encoded antisense rna associated with the replication process of salmonella enterica serovar typhi.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2225266534ef48ff8c7f326e494d0844
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Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.

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