Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain

The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype a...

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Autores principales: Richard Duc Hien Tran, Tessa Altair Morris, Daniela Gonzalez, Ali Hatem Salaheldin Hassan Ahmed Hetta, Anna Grosberg
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:22261ae217ab43078f7ab74e0860efd72021-11-25T17:12:47ZQuantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain10.3390/cells101131992073-4409https://doaj.org/article/22261ae217ab43078f7ab74e0860efd72021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3199https://doaj.org/toc/2073-4409The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.Richard Duc Hien TranTessa Altair MorrisDaniela GonzalezAli Hatem Salaheldin Hassan Ahmed HettaAnna GrosbergMDPI AGarticleheart tissue organizationcell type classificationcyclic strainintercellular junctionsBiology (General)QH301-705.5ENCells, Vol 10, Iss 3199, p 3199 (2021)
institution DOAJ
collection DOAJ
language EN
topic heart tissue organization
cell type classification
cyclic strain
intercellular junctions
Biology (General)
QH301-705.5
spellingShingle heart tissue organization
cell type classification
cyclic strain
intercellular junctions
Biology (General)
QH301-705.5
Richard Duc Hien Tran
Tessa Altair Morris
Daniela Gonzalez
Ali Hatem Salaheldin Hassan Ahmed Hetta
Anna Grosberg
Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
description The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.
format article
author Richard Duc Hien Tran
Tessa Altair Morris
Daniela Gonzalez
Ali Hatem Salaheldin Hassan Ahmed Hetta
Anna Grosberg
author_facet Richard Duc Hien Tran
Tessa Altair Morris
Daniela Gonzalez
Ali Hatem Salaheldin Hassan Ahmed Hetta
Anna Grosberg
author_sort Richard Duc Hien Tran
title Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_short Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_full Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_fullStr Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_full_unstemmed Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_sort quantitative evaluation of cardiac cell interactions and responses to cyclic strain
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/22261ae217ab43078f7ab74e0860efd7
work_keys_str_mv AT richardduchientran quantitativeevaluationofcardiaccellinteractionsandresponsestocyclicstrain
AT tessaaltairmorris quantitativeevaluationofcardiaccellinteractionsandresponsestocyclicstrain
AT danielagonzalez quantitativeevaluationofcardiaccellinteractionsandresponsestocyclicstrain
AT alihatemsalaheldinhassanahmedhetta quantitativeevaluationofcardiaccellinteractionsandresponsestocyclicstrain
AT annagrosberg quantitativeevaluationofcardiaccellinteractionsandresponsestocyclicstrain
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