Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain

The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype a...

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Autores principales:	Richard Duc Hien Tran, Tessa Altair Morris, Daniela Gonzalez, Ali Hatem Salaheldin Hassan Ahmed Hetta, Anna Grosberg
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	heart tissue organization cell type classification cyclic strain intercellular junctions Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/22261ae217ab43078f7ab74e0860efd7
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spelling	oai:doaj.org-article:22261ae217ab43078f7ab74e0860efd72021-11-25T17:12:47ZQuantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain10.3390/cells101131992073-4409https://doaj.org/article/22261ae217ab43078f7ab74e0860efd72021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3199https://doaj.org/toc/2073-4409The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.Richard Duc Hien TranTessa Altair MorrisDaniela GonzalezAli Hatem Salaheldin Hassan Ahmed HettaAnna GrosbergMDPI AGarticleheart tissue organizationcell type classificationcyclic strainintercellular junctionsBiology (General)QH301-705.5ENCells, Vol 10, Iss 3199, p 3199 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heart tissue organization cell type classification cyclic strain intercellular junctions Biology (General) QH301-705.5
spellingShingle	heart tissue organization cell type classification cyclic strain intercellular junctions Biology (General) QH301-705.5 Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
description	The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.
format	article
author	Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg
author_facet	Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg
author_sort	Richard Duc Hien Tran
title	Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_short	Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_full	Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_fullStr	Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_full_unstemmed	Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
title_sort	quantitative evaluation of cardiac cell interactions and responses to cyclic strain
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/22261ae217ab43078f7ab74e0860efd7
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Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain

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