Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement

Abstract During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior...

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Autores principales:	Carla Daniela Cisternas, Lucas Ezequiel Cabrera Zapata, María Angeles Arevalo, Luis Miguel Garcia-Segura, María Julia Cambiasso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2228067e4a1b4f5ab88a38099db10d23
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spelling	oai:doaj.org-article:2228067e4a1b4f5ab88a38099db10d232021-12-02T16:06:23ZRegulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement10.1038/s41598-017-05658-62045-2322https://doaj.org/article/2228067e4a1b4f5ab88a38099db10d232017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05658-6https://doaj.org/toc/2045-2322Abstract During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior amygdala neuronal cultures expressed higher levels of aromatase (mRNA and protein) than female cultures. Furthermore, treatment with oestradiol (E2) or dihydrotestosterone (DHT) increased Cyp19a1 expression and aromatase protein levels only in female neuronal cultures. The effect of E2 on aromatase expression was not imitated by oestrogen receptor (ER) α agonist PPT or the GPER agonist G1, but it was fully reproduced by DPN, a specific ligand of ERβ. By contrast, the effect of DHT on aromatase expression was not blocked by the anti-androgen flutamide, but completely abrogated by the ERβ antagonist PHTPP. Experiments using the four core genotype model showed a sex chromosome effect in ERβ expression (XY > XX) and regulation by E2 or DHT (only XX respond) in amygdala neurons. In conclusion, sex chromosome complement governs the hormonal regulation of aromatase expression through activation of ERβ in developing mouse brain.Carla Daniela CisternasLucas Ezequiel Cabrera ZapataMaría Angeles ArevaloLuis Miguel Garcia-SeguraMaría Julia CambiassoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carla Daniela Cisternas Lucas Ezequiel Cabrera Zapata María Angeles Arevalo Luis Miguel Garcia-Segura María Julia Cambiasso Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
description	Abstract During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior amygdala neuronal cultures expressed higher levels of aromatase (mRNA and protein) than female cultures. Furthermore, treatment with oestradiol (E2) or dihydrotestosterone (DHT) increased Cyp19a1 expression and aromatase protein levels only in female neuronal cultures. The effect of E2 on aromatase expression was not imitated by oestrogen receptor (ER) α agonist PPT or the GPER agonist G1, but it was fully reproduced by DPN, a specific ligand of ERβ. By contrast, the effect of DHT on aromatase expression was not blocked by the anti-androgen flutamide, but completely abrogated by the ERβ antagonist PHTPP. Experiments using the four core genotype model showed a sex chromosome effect in ERβ expression (XY > XX) and regulation by E2 or DHT (only XX respond) in amygdala neurons. In conclusion, sex chromosome complement governs the hormonal regulation of aromatase expression through activation of ERβ in developing mouse brain.
format	article
author	Carla Daniela Cisternas Lucas Ezequiel Cabrera Zapata María Angeles Arevalo Luis Miguel Garcia-Segura María Julia Cambiasso
author_facet	Carla Daniela Cisternas Lucas Ezequiel Cabrera Zapata María Angeles Arevalo Luis Miguel Garcia-Segura María Julia Cambiasso
author_sort	Carla Daniela Cisternas
title	Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
title_short	Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
title_full	Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
title_fullStr	Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
title_full_unstemmed	Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement
title_sort	regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on erβ and sex chromosome complement
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2228067e4a1b4f5ab88a38099db10d23
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Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERβ and sex chromosome complement

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