Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats

BACKGROUND AND OBJECTIVE: Herbal medicine had been excessively used for treatment of many diseases in traditional medicine. Because of effect of fructose on lipid profile and hepatic enzymes, it was decided to study the effect of extract of different parts of Citrullus colocynthis on lipid profile a...

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Autores principales: A Ahangarpour, AA Oroojan
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Publicado: Babol University of Medical Sciences 2012
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spelling oai:doaj.org-article:222a70384edd4f628191bc661f4e56a32021-11-10T08:53:19ZEffect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats1561-41072251-7170https://doaj.org/article/222a70384edd4f628191bc661f4e56a32012-07-01T00:00:00Zhttp://jbums.org/article-1-4154-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Herbal medicine had been excessively used for treatment of many diseases in traditional medicine. Because of effect of fructose on lipid profile and hepatic enzymes, it was decided to study the effect of extract of different parts of Citrullus colocynthis on lipid profile and hepatic enzymes in fructose-fed male rats. METHODS: In this experimental study, 63 adult male Wistar rats weighting 200-300 (g) were used. After fructose 10% induced in drinking water for 8 weeks, the animals were randomly divided into 7 groups: control, Fructose10%, aqueous extracts of seed, hydro-alcoholic extracts of seed, aqueous extracts of crust, hydro-alcoholic extracts of crust and hydro-alcoholic extracts of pulp. The extracts (200mg/kg) were administered orally and conducted for 2 terminal weeks. The animals were weighed and anesthetized for the serum blood collection to determine lipid profile and hepatic enzymes after at least a 12-h fast with manufacture kits and autoanalyzer machine. FINDINGS: LDL/HDL ratio in aqueous extracts of crust group (2.46±1.08) increased in comparison with control and fructose groups (0.98±0.1 and 0.98± 0.15, respectively) (p<0.01). Aspartate aminotransferase (AST) in aqueous extracts of seed and aqueous extracts of crust groups decreased and increased in comparison with control and fructose groups, respectively (p<0.05). Alanine aminotransferase (ALT) in aqueous extracts of crust group increased in comparison with control and fructose groups (p<0.01), also alkaline phosphatase (ALP) in aqueous extracts of crust group increased (p<0.05) but this enzyme in aqueous extracts of seed group decreased significantly in comparison with control (p<0.01) and fructose (p<0.05) group. CONCLUSION: According to the results, crust of Citrullus colocynthis has negative effect on lipid profile, hepatic enzymes and hepatotoxicity and seed of this fruit has decrease effect on lipid profile and hepatic enzymes.A AhangarpourAA OroojanBabol University of Medical Sciencesarticlecitrullus colocynthisfructoselipidhepatic enzymeMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 4, Pp 53-60 (2012)
institution DOAJ
collection DOAJ
language EN
FA
topic citrullus colocynthis
fructose
lipid
hepatic enzyme
Medicine
R
Medicine (General)
R5-920
spellingShingle citrullus colocynthis
fructose
lipid
hepatic enzyme
Medicine
R
Medicine (General)
R5-920
A Ahangarpour
AA Oroojan
Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
description BACKGROUND AND OBJECTIVE: Herbal medicine had been excessively used for treatment of many diseases in traditional medicine. Because of effect of fructose on lipid profile and hepatic enzymes, it was decided to study the effect of extract of different parts of Citrullus colocynthis on lipid profile and hepatic enzymes in fructose-fed male rats. METHODS: In this experimental study, 63 adult male Wistar rats weighting 200-300 (g) were used. After fructose 10% induced in drinking water for 8 weeks, the animals were randomly divided into 7 groups: control, Fructose10%, aqueous extracts of seed, hydro-alcoholic extracts of seed, aqueous extracts of crust, hydro-alcoholic extracts of crust and hydro-alcoholic extracts of pulp. The extracts (200mg/kg) were administered orally and conducted for 2 terminal weeks. The animals were weighed and anesthetized for the serum blood collection to determine lipid profile and hepatic enzymes after at least a 12-h fast with manufacture kits and autoanalyzer machine. FINDINGS: LDL/HDL ratio in aqueous extracts of crust group (2.46±1.08) increased in comparison with control and fructose groups (0.98±0.1 and 0.98± 0.15, respectively) (p<0.01). Aspartate aminotransferase (AST) in aqueous extracts of seed and aqueous extracts of crust groups decreased and increased in comparison with control and fructose groups, respectively (p<0.05). Alanine aminotransferase (ALT) in aqueous extracts of crust group increased in comparison with control and fructose groups (p<0.01), also alkaline phosphatase (ALP) in aqueous extracts of crust group increased (p<0.05) but this enzyme in aqueous extracts of seed group decreased significantly in comparison with control (p<0.01) and fructose (p<0.05) group. CONCLUSION: According to the results, crust of Citrullus colocynthis has negative effect on lipid profile, hepatic enzymes and hepatotoxicity and seed of this fruit has decrease effect on lipid profile and hepatic enzymes.
format article
author A Ahangarpour
AA Oroojan
author_facet A Ahangarpour
AA Oroojan
author_sort A Ahangarpour
title Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
title_short Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
title_full Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
title_fullStr Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
title_full_unstemmed Effect of Crust and Seed’s Aqueous Extract and Hydro-Alcoholic Extracts of Crust, Seed and Pulp of Citrullus Colocynthis on Lipid’s Factors and Hepatic Enzyme in Fructose-Fed Male Rats
title_sort effect of crust and seed’s aqueous extract and hydro-alcoholic extracts of crust, seed and pulp of citrullus colocynthis on lipid’s factors and hepatic enzyme in fructose-fed male rats
publisher Babol University of Medical Sciences
publishDate 2012
url https://doaj.org/article/222a70384edd4f628191bc661f4e56a3
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