The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses

Horses have one of the highest skeletal muscle oxidative capacities amongst mammals, which, combined with a high glycolytic capacity, could perturb redox status during maximal exercise. We determined the effect of 30 d of oral coenzyme Q10 and N-acetyl-cysteine supplementation (NACQ) on muscle gluta...

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Autores principales:	Marisa L. Henry, Deborah Velez-Irizarry, Joe D. Pagan, Lorraine Sordillo, Jeff Gandy, Stephanie J. Valberg
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidants glycolysis mitochondria mitochondrial proteins reactive oxygen species redox Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/222dbc35720e4e31bbb63ffc0efea19f
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spelling	oai:doaj.org-article:222dbc35720e4e31bbb63ffc0efea19f2021-11-25T16:27:34ZThe Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses10.3390/antiox101117392076-3921https://doaj.org/article/222dbc35720e4e31bbb63ffc0efea19f2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1739https://doaj.org/toc/2076-3921Horses have one of the highest skeletal muscle oxidative capacities amongst mammals, which, combined with a high glycolytic capacity, could perturb redox status during maximal exercise. We determined the effect of 30 d of oral coenzyme Q10 and N-acetyl-cysteine supplementation (NACQ) on muscle glutathione (GSH), cysteine, ROS, and coenzyme Q10 concentrations, and the muscle proteome, in seven maximally exercising Thoroughbred horses using a placebo and randomized cross-over design. Gluteal muscle biopsies were obtained the day before and 1 h after maximal exercise. Concentrations of GSH, cysteine, coenzyme Q10, and ROS were measured, and citrate synthase, glutathione peroxidase, and superoxide dismutase activities analyzed. GSH increased significantly 1 h post-exercise in the NACQ group (<i>p</i> = 0.022), whereas other antioxidant concentrations/activities were unchanged. TMT proteomic analysis revealed 40 differentially expressed proteins with NACQ out of 387 identified, including upregulation of 13 mitochondrial proteins (TCA cycle and NADPH production), 4 Z-disc proteins, and down regulation of 9 glycolytic proteins. NACQ supplementation significantly impacted muscle redox capacity after intense exercise by enhancing muscle glutathione concentrations and increasing expression of proteins involved in the uptake of glutathione into mitochondria and the NAPDH-associated reduction of oxidized glutathione, without any evident detrimental effects on performance.Marisa L. HenryDeborah Velez-IrizarryJoe D. PaganLorraine SordilloJeff GandyStephanie J. ValbergMDPI AGarticleantioxidantsglycolysismitochondriamitochondrial proteinsreactive oxygen speciesredoxTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1739, p 1739 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidants glycolysis mitochondria mitochondrial proteins reactive oxygen species redox Therapeutics. Pharmacology RM1-950
spellingShingle	antioxidants glycolysis mitochondria mitochondrial proteins reactive oxygen species redox Therapeutics. Pharmacology RM1-950 Marisa L. Henry Deborah Velez-Irizarry Joe D. Pagan Lorraine Sordillo Jeff Gandy Stephanie J. Valberg The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
description	Horses have one of the highest skeletal muscle oxidative capacities amongst mammals, which, combined with a high glycolytic capacity, could perturb redox status during maximal exercise. We determined the effect of 30 d of oral coenzyme Q10 and N-acetyl-cysteine supplementation (NACQ) on muscle glutathione (GSH), cysteine, ROS, and coenzyme Q10 concentrations, and the muscle proteome, in seven maximally exercising Thoroughbred horses using a placebo and randomized cross-over design. Gluteal muscle biopsies were obtained the day before and 1 h after maximal exercise. Concentrations of GSH, cysteine, coenzyme Q10, and ROS were measured, and citrate synthase, glutathione peroxidase, and superoxide dismutase activities analyzed. GSH increased significantly 1 h post-exercise in the NACQ group (<i>p</i> = 0.022), whereas other antioxidant concentrations/activities were unchanged. TMT proteomic analysis revealed 40 differentially expressed proteins with NACQ out of 387 identified, including upregulation of 13 mitochondrial proteins (TCA cycle and NADPH production), 4 Z-disc proteins, and down regulation of 9 glycolytic proteins. NACQ supplementation significantly impacted muscle redox capacity after intense exercise by enhancing muscle glutathione concentrations and increasing expression of proteins involved in the uptake of glutathione into mitochondria and the NAPDH-associated reduction of oxidized glutathione, without any evident detrimental effects on performance.
format	article
author	Marisa L. Henry Deborah Velez-Irizarry Joe D. Pagan Lorraine Sordillo Jeff Gandy Stephanie J. Valberg
author_facet	Marisa L. Henry Deborah Velez-Irizarry Joe D. Pagan Lorraine Sordillo Jeff Gandy Stephanie J. Valberg
author_sort	Marisa L. Henry
title	The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
title_short	The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
title_full	The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
title_fullStr	The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
title_full_unstemmed	The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses
title_sort	impact of n-acetyl cysteine and coenzyme q10 supplementation on skeletal muscle antioxidants and proteome in fit thoroughbred horses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/222dbc35720e4e31bbb63ffc0efea19f
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The Impact of N-Acetyl Cysteine and Coenzyme Q10 Supplementation on Skeletal Muscle Antioxidants and Proteome in Fit Thoroughbred Horses

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