Computational and genetic reduction of a cell cycle to its simplest, primordial components.

What are the minimal requirements to sustain an asymmetric cell cycle? Here we use mathematical modelling and forward genetics to reduce an asymmetric cell cycle to its simplest, primordial components. In the Alphaproteobacterium Caulobacter crescentus, cell cycle progression is believed to be contr...

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Autores principales:	Seán M Murray, Gaël Panis, Coralie Fumeaux, Patrick H Viollier, Martin Howard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/22398489374749cbbcb4725d35434835
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spelling	oai:doaj.org-article:22398489374749cbbcb4725d354348352021-11-18T05:37:39ZComputational and genetic reduction of a cell cycle to its simplest, primordial components.1544-91731545-788510.1371/journal.pbio.1001749https://doaj.org/article/22398489374749cbbcb4725d354348352013-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24415923/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885What are the minimal requirements to sustain an asymmetric cell cycle? Here we use mathematical modelling and forward genetics to reduce an asymmetric cell cycle to its simplest, primordial components. In the Alphaproteobacterium Caulobacter crescentus, cell cycle progression is believed to be controlled by a cyclical genetic circuit comprising four essential master regulators. Unexpectedly, our in silico modelling predicted that one of these regulators, GcrA, is in fact dispensable. We confirmed this experimentally, finding that ΔgcrA cells are viable, but slow-growing and elongated, with the latter mostly due to an insufficiency of a key cell division protein. Furthermore, suppressor analysis showed that another cell cycle regulator, the methyltransferase CcrM, is similarly dispensable with simultaneous gcrA/ccrM disruption ameliorating the cytokinetic and growth defect of ΔgcrA cells. Within the Alphaproteobacteria, gcrA and ccrM are consistently present or absent together, rather than either gene being present alone, suggesting that gcrA/ccrM constitutes an independent, dispensable genetic module. Together our approaches unveil the essential elements of a primordial asymmetric cell cycle that should help illuminate more complex cell cycles.Seán M MurrayGaël PanisCoralie FumeauxPatrick H ViollierMartin HowardPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 12, p e1001749 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Seán M Murray Gaël Panis Coralie Fumeaux Patrick H Viollier Martin Howard Computational and genetic reduction of a cell cycle to its simplest, primordial components.
description	What are the minimal requirements to sustain an asymmetric cell cycle? Here we use mathematical modelling and forward genetics to reduce an asymmetric cell cycle to its simplest, primordial components. In the Alphaproteobacterium Caulobacter crescentus, cell cycle progression is believed to be controlled by a cyclical genetic circuit comprising four essential master regulators. Unexpectedly, our in silico modelling predicted that one of these regulators, GcrA, is in fact dispensable. We confirmed this experimentally, finding that ΔgcrA cells are viable, but slow-growing and elongated, with the latter mostly due to an insufficiency of a key cell division protein. Furthermore, suppressor analysis showed that another cell cycle regulator, the methyltransferase CcrM, is similarly dispensable with simultaneous gcrA/ccrM disruption ameliorating the cytokinetic and growth defect of ΔgcrA cells. Within the Alphaproteobacteria, gcrA and ccrM are consistently present or absent together, rather than either gene being present alone, suggesting that gcrA/ccrM constitutes an independent, dispensable genetic module. Together our approaches unveil the essential elements of a primordial asymmetric cell cycle that should help illuminate more complex cell cycles.
format	article
author	Seán M Murray Gaël Panis Coralie Fumeaux Patrick H Viollier Martin Howard
author_facet	Seán M Murray Gaël Panis Coralie Fumeaux Patrick H Viollier Martin Howard
author_sort	Seán M Murray
title	Computational and genetic reduction of a cell cycle to its simplest, primordial components.
title_short	Computational and genetic reduction of a cell cycle to its simplest, primordial components.
title_full	Computational and genetic reduction of a cell cycle to its simplest, primordial components.
title_fullStr	Computational and genetic reduction of a cell cycle to its simplest, primordial components.
title_full_unstemmed	Computational and genetic reduction of a cell cycle to its simplest, primordial components.
title_sort	computational and genetic reduction of a cell cycle to its simplest, primordial components.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/22398489374749cbbcb4725d35434835
work_keys_str_mv	AT seanmmurray computationalandgeneticreductionofacellcycletoitssimplestprimordialcomponents AT gaelpanis computationalandgeneticreductionofacellcycletoitssimplestprimordialcomponents AT coraliefumeaux computationalandgeneticreductionofacellcycletoitssimplestprimordialcomponents AT patrickhviollier computationalandgeneticreductionofacellcycletoitssimplestprimordialcomponents AT martinhoward computationalandgeneticreductionofacellcycletoitssimplestprimordialcomponents
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Computational and genetic reduction of a cell cycle to its simplest, primordial components.

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