A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography

Abstract Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging sui...

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Autores principales: Maria Pierantoni, Isabella Silva Barreto, Malin Hammerman, Lissa Verhoeven, Elin Törnquist, Vladimir Novak, Rajmund Mokso, Pernilla Eliasson, Hanna Isaksson
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/223b4f45c3e9416fa0e52b373efb3a66
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spelling oai:doaj.org-article:223b4f45c3e9416fa0e52b373efb3a662021-12-02T16:35:11ZA quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography10.1038/s41598-021-96589-w2045-2322https://doaj.org/article/223b4f45c3e9416fa0e52b373efb3a662021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96589-whttps://doaj.org/toc/2045-2322Abstract Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.Maria PierantoniIsabella Silva BarretoMalin HammermanLissa VerhoevenElin TörnquistVladimir NovakRajmund MoksoPernilla EliassonHanna IsakssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Maria Pierantoni
Isabella Silva Barreto
Malin Hammerman
Lissa Verhoeven
Elin Törnquist
Vladimir Novak
Rajmund Mokso
Pernilla Eliasson
Hanna Isaksson
A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
description Abstract Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.
format article
author Maria Pierantoni
Isabella Silva Barreto
Malin Hammerman
Lissa Verhoeven
Elin Törnquist
Vladimir Novak
Rajmund Mokso
Pernilla Eliasson
Hanna Isaksson
author_facet Maria Pierantoni
Isabella Silva Barreto
Malin Hammerman
Lissa Verhoeven
Elin Törnquist
Vladimir Novak
Rajmund Mokso
Pernilla Eliasson
Hanna Isaksson
author_sort Maria Pierantoni
title A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
title_short A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
title_full A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
title_fullStr A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
title_full_unstemmed A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
title_sort quality optimization approach to image achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/223b4f45c3e9416fa0e52b373efb3a66
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