Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability

Wound healing is related to proliferation, migration and angiogenesis of keratinocytes. Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) is an important N6-methyladenosine (m6A) reader, which is involved in multiple processes, including wound healing. However, the function and mechanism...

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Autores principales:	Shaomin Zhi, Jun Li, Xiao Kong, Xuemei Xie, Qiangli Zhang, Guoxiang Fang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	wound healing keratinocyte igf2bp2 hpse proliferation migration angiogenesis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2245524e540847f28fafda5bda7d2a59
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spelling	oai:doaj.org-article:2245524e540847f28fafda5bda7d2a592021-11-11T14:23:43ZInsulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability2165-59792165-598710.1080/21655979.2021.2002495https://doaj.org/article/2245524e540847f28fafda5bda7d2a592021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2002495https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Wound healing is related to proliferation, migration and angiogenesis of keratinocytes. Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) is an important N6-methyladenosine (m6A) reader, which is involved in multiple processes, including wound healing. However, the function and mechanism of IGF2BP2 in keratinocyte processes are largely uncertain. In the present study, expression levels of IGF2BP2 and heparanase (HPSE) were detected by quantitative reverse transcription polymerase chain reaction and western blotting assays. Cell proliferation was investigated with cell counting kit-8 analysis. Cell migration was determined through wound healing assay. Angiogenesis was measured by tube formation assay and vascular endothelial growth factor (VEGF) level using enzyme linked immunosorbent assay. The interaction between IGF2BP2 and HPSE was analyzed by RNA immunoprecipitation, pull-down and luciferase reporter analyses. The results showed that IGF2BP2 expression was enhanced in wound healing. IGF2BP2 downregulation constrained HaCaT cell proliferation, migration and angiogenesis. IGF2BP2 knockdown decreased HPSE expression. IGF2BP2 could regulate HPSE stability by binding with 3ʹ untranslated region (UTR) of HPSE. HPSE upregulation attenuated silencing IGF2BP2-mediated suppression of proliferation, migration and angiogenesis. As a conclusion, IGF2BP2 knockdown repressed proliferation, migration and angiogenesis of HaCaT cells by decreasing HPSE stability.Shaomin ZhiJun LiXiao KongXuemei XieQiangli ZhangGuoxiang FangTaylor & Francis Grouparticlewound healingkeratinocyteigf2bp2hpseproliferationmigrationangiogenesisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wound healing keratinocyte igf2bp2 hpse proliferation migration angiogenesis Biotechnology TP248.13-248.65
spellingShingle	wound healing keratinocyte igf2bp2 hpse proliferation migration angiogenesis Biotechnology TP248.13-248.65 Shaomin Zhi Jun Li Xiao Kong Xuemei Xie Qiangli Zhang Guoxiang Fang Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
description	Wound healing is related to proliferation, migration and angiogenesis of keratinocytes. Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) is an important N6-methyladenosine (m6A) reader, which is involved in multiple processes, including wound healing. However, the function and mechanism of IGF2BP2 in keratinocyte processes are largely uncertain. In the present study, expression levels of IGF2BP2 and heparanase (HPSE) were detected by quantitative reverse transcription polymerase chain reaction and western blotting assays. Cell proliferation was investigated with cell counting kit-8 analysis. Cell migration was determined through wound healing assay. Angiogenesis was measured by tube formation assay and vascular endothelial growth factor (VEGF) level using enzyme linked immunosorbent assay. The interaction between IGF2BP2 and HPSE was analyzed by RNA immunoprecipitation, pull-down and luciferase reporter analyses. The results showed that IGF2BP2 expression was enhanced in wound healing. IGF2BP2 downregulation constrained HaCaT cell proliferation, migration and angiogenesis. IGF2BP2 knockdown decreased HPSE expression. IGF2BP2 could regulate HPSE stability by binding with 3ʹ untranslated region (UTR) of HPSE. HPSE upregulation attenuated silencing IGF2BP2-mediated suppression of proliferation, migration and angiogenesis. As a conclusion, IGF2BP2 knockdown repressed proliferation, migration and angiogenesis of HaCaT cells by decreasing HPSE stability.
format	article
author	Shaomin Zhi Jun Li Xiao Kong Xuemei Xie Qiangli Zhang Guoxiang Fang
author_facet	Shaomin Zhi Jun Li Xiao Kong Xuemei Xie Qiangli Zhang Guoxiang Fang
author_sort	Shaomin Zhi
title	Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
title_short	Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
title_full	Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
title_fullStr	Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
title_full_unstemmed	Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
title_sort	insulin-like growth factor 2 mrna binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2245524e540847f28fafda5bda7d2a59
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_version_	1718438919916224512

Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability

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