Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility.
Meiosis is essential for the generation of gametes and sexual reproduction, yet the factors and underlying mechanisms regulating meiotic progression remain largely unknown. Here, we showed that MTL5 translocates into nuclei of spermatocytes during zygotene-pachytene transition and ensures meiosis ad...
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Public Library of Science (PLoS)
2021
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oai:doaj.org-article:22465a6b721242288bb3e3dee9e687bc2021-12-02T20:02:52ZNuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility.1553-73901553-740410.1371/journal.pgen.1009753https://doaj.org/article/22465a6b721242288bb3e3dee9e687bc2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009753https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Meiosis is essential for the generation of gametes and sexual reproduction, yet the factors and underlying mechanisms regulating meiotic progression remain largely unknown. Here, we showed that MTL5 translocates into nuclei of spermatocytes during zygotene-pachytene transition and ensures meiosis advances beyond pachytene stage. MTL5 shows strong interactions with MuvB core complex components, a well-known transcriptional complex regulating mitotic progression, and the zygotene-pachytene transition of MTL5 is mediated by its direct interaction with the component LIN9, through MTL5 C-terminal 443-475 residues. Male Mtl5c-mu/c-mu mice expressing the truncated MTL5 (p.Ser445Arg fs*3) that lacks the interaction with LIN9 and is detained in cytoplasm showed male infertility and spermatogenic arrest at pachytene stage, same as that of Mtl5 knockout mice, indicating that the interaction with LIN9 is essential for the nuclear translocation and function of MTL5 during meiosis. Our data demonstrated MTL5 translocates into nuclei during the zygotene-pachytene transition to initiate its function along with the MuvB core complex in pachytene spermatocytes, highlighting a new mechanism regulating the progression of male meiosis.Xingxia ZhangMing LiXiaohua JiangHui MaSuixing FanYang LiChangping YuJianze XuRanjha KhanHanwei JiangQinghua ShiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009753 (2021) |
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Genetics QH426-470 |
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Genetics QH426-470 Xingxia Zhang Ming Li Xiaohua Jiang Hui Ma Suixing Fan Yang Li Changping Yu Jianze Xu Ranjha Khan Hanwei Jiang Qinghua Shi Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| description |
Meiosis is essential for the generation of gametes and sexual reproduction, yet the factors and underlying mechanisms regulating meiotic progression remain largely unknown. Here, we showed that MTL5 translocates into nuclei of spermatocytes during zygotene-pachytene transition and ensures meiosis advances beyond pachytene stage. MTL5 shows strong interactions with MuvB core complex components, a well-known transcriptional complex regulating mitotic progression, and the zygotene-pachytene transition of MTL5 is mediated by its direct interaction with the component LIN9, through MTL5 C-terminal 443-475 residues. Male Mtl5c-mu/c-mu mice expressing the truncated MTL5 (p.Ser445Arg fs*3) that lacks the interaction with LIN9 and is detained in cytoplasm showed male infertility and spermatogenic arrest at pachytene stage, same as that of Mtl5 knockout mice, indicating that the interaction with LIN9 is essential for the nuclear translocation and function of MTL5 during meiosis. Our data demonstrated MTL5 translocates into nuclei during the zygotene-pachytene transition to initiate its function along with the MuvB core complex in pachytene spermatocytes, highlighting a new mechanism regulating the progression of male meiosis. |
| format |
article |
| author |
Xingxia Zhang Ming Li Xiaohua Jiang Hui Ma Suixing Fan Yang Li Changping Yu Jianze Xu Ranjha Khan Hanwei Jiang Qinghua Shi |
| author_facet |
Xingxia Zhang Ming Li Xiaohua Jiang Hui Ma Suixing Fan Yang Li Changping Yu Jianze Xu Ranjha Khan Hanwei Jiang Qinghua Shi |
| author_sort |
Xingxia Zhang |
| title |
Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| title_short |
Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| title_full |
Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| title_fullStr |
Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| title_full_unstemmed |
Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. |
| title_sort |
nuclear translocation of mtl5 from cytoplasm requires its direct interaction with lin9 and is essential for male meiosis and fertility. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/22465a6b721242288bb3e3dee9e687bc |
| work_keys_str_mv |
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