Identification of a novel human polyomavirus in organs of the gastrointestinal tract.
Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HP...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/22517350867e4f7a98c80b37c7cb1e60 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:22517350867e4f7a98c80b37c7cb1e60 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:22517350867e4f7a98c80b37c7cb1e602021-11-18T07:53:42ZIdentification of a novel human polyomavirus in organs of the gastrointestinal tract.1932-620310.1371/journal.pone.0058021https://doaj.org/article/22517350867e4f7a98c80b37c7cb1e602013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516426/?tool=EBIhttps://doaj.org/toc/1932-6203Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23% and 17%, respectively, were determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus naturally infects humans and that primary infection may already occur in childhood.Sarah KorupJanita RietscherSébastien Calvignac-SpencerFranziska TruschJörg HofmannUgo MoensIgor SauerSebastian VoigtRosa SchmuckBernhard EhlersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58021 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Sarah Korup Janita Rietscher Sébastien Calvignac-Spencer Franziska Trusch Jörg Hofmann Ugo Moens Igor Sauer Sebastian Voigt Rosa Schmuck Bernhard Ehlers Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| description |
Polyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23% and 17%, respectively, were determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus naturally infects humans and that primary infection may already occur in childhood. |
| format |
article |
| author |
Sarah Korup Janita Rietscher Sébastien Calvignac-Spencer Franziska Trusch Jörg Hofmann Ugo Moens Igor Sauer Sebastian Voigt Rosa Schmuck Bernhard Ehlers |
| author_facet |
Sarah Korup Janita Rietscher Sébastien Calvignac-Spencer Franziska Trusch Jörg Hofmann Ugo Moens Igor Sauer Sebastian Voigt Rosa Schmuck Bernhard Ehlers |
| author_sort |
Sarah Korup |
| title |
Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| title_short |
Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| title_full |
Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| title_fullStr |
Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| title_full_unstemmed |
Identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| title_sort |
identification of a novel human polyomavirus in organs of the gastrointestinal tract. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/22517350867e4f7a98c80b37c7cb1e60 |
| work_keys_str_mv |
AT sarahkorup identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT janitarietscher identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT sebastiencalvignacspencer identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT franziskatrusch identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT jorghofmann identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT ugomoens identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT igorsauer identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT sebastianvoigt identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT rosaschmuck identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract AT bernhardehlers identificationofanovelhumanpolyomavirusinorgansofthegastrointestinaltract |
| _version_ |
1718422774416932864 |