The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.

Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression. High-resolution mapping has recently identified hundreds of large, sharply defined lamina-associated domains (LADs) in...

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Autores principales:	Joke G van Bemmel, Ludo Pagie, Ulrich Braunschweig, Wim Brugman, Wouter Meuleman, Ron M Kerkhoven, Bas van Steensel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2255ab47558f499e9a9e94d762947b29
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spelling	oai:doaj.org-article:2255ab47558f499e9a9e94d762947b292021-11-18T07:36:33ZThe insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.1932-620310.1371/journal.pone.0015013https://doaj.org/article/2255ab47558f499e9a9e94d762947b292010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21124834/?tool=EBIhttps://doaj.org/toc/1932-6203Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression. High-resolution mapping has recently identified hundreds of large, sharply defined lamina-associated domains (LADs) in the human genome, and suggested that the insulator protein CTCF may help to demarcate these domains. Here, we report the detailed structure of LADs in Drosophila cells, and investigate the putative roles of five insulator proteins in LAD organization. We found that the Drosophila genome is also organized in discrete LADs, which are about five times smaller than human LADs but contain on average a similar number of genes. Systematic comparison to new and published insulator binding maps shows that only SU(HW) binds preferentially at LAD borders and at specific positions inside LADs, while GAF, CTCF, BEAF-32 and DWG are mostly absent from these regions. By knockdown and overexpression studies we demonstrate that SU(HW) weakens genome - NL interactions through a local antagonistic effect, but we did not obtain evidence that it is essential for border formation. Our results provide insights into the evolution of LAD organization and identify SU(HW) as a fine-tuner of genome - NL interactions.Joke G van BemmelLudo PagieUlrich BraunschweigWim BrugmanWouter MeulemanRon M KerkhovenBas van SteenselPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 11, p e15013 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joke G van Bemmel Ludo Pagie Ulrich Braunschweig Wim Brugman Wouter Meuleman Ron M Kerkhoven Bas van Steensel The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
description	Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression. High-resolution mapping has recently identified hundreds of large, sharply defined lamina-associated domains (LADs) in the human genome, and suggested that the insulator protein CTCF may help to demarcate these domains. Here, we report the detailed structure of LADs in Drosophila cells, and investigate the putative roles of five insulator proteins in LAD organization. We found that the Drosophila genome is also organized in discrete LADs, which are about five times smaller than human LADs but contain on average a similar number of genes. Systematic comparison to new and published insulator binding maps shows that only SU(HW) binds preferentially at LAD borders and at specific positions inside LADs, while GAF, CTCF, BEAF-32 and DWG are mostly absent from these regions. By knockdown and overexpression studies we demonstrate that SU(HW) weakens genome - NL interactions through a local antagonistic effect, but we did not obtain evidence that it is essential for border formation. Our results provide insights into the evolution of LAD organization and identify SU(HW) as a fine-tuner of genome - NL interactions.
format	article
author	Joke G van Bemmel Ludo Pagie Ulrich Braunschweig Wim Brugman Wouter Meuleman Ron M Kerkhoven Bas van Steensel
author_facet	Joke G van Bemmel Ludo Pagie Ulrich Braunschweig Wim Brugman Wouter Meuleman Ron M Kerkhoven Bas van Steensel
author_sort	Joke G van Bemmel
title	The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
title_short	The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
title_full	The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
title_fullStr	The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
title_full_unstemmed	The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.
title_sort	insulator protein su(hw) fine-tunes nuclear lamina interactions of the drosophila genome.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/2255ab47558f499e9a9e94d762947b29
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The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.

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