Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats

Probiotics administration can facilitate the restoration of host gut microbiota/metabolome after antibiotic treatment. Yet, the mechanism behind such beneficial effects remains unclear. This study constructed a rat model of antibiotic-induced gut dysbiosis to monitor the effects and mechanism of pro...

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Autores principales:	Guoqiang Yao, Chenxia Cao, Meng Zhang, Lai-Yu Kwok, Heping Zhang, Wenyi Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Lactobacillus casei Zhang Cefdinir Antibiotic resistance gene (ARG) Parabacteroides goldsteinii Short-chain fatty acids IL-1α Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2258c5f07b3c47dfbd17ffe11b6b76c9
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spelling	oai:doaj.org-article:2258c5f07b3c47dfbd17ffe11b6b76c92021-11-04T04:28:08ZLactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats2001-037010.1016/j.csbj.2021.10.026https://doaj.org/article/2258c5f07b3c47dfbd17ffe11b6b76c92021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004487https://doaj.org/toc/2001-0370Probiotics administration can facilitate the restoration of host gut microbiota/metabolome after antibiotic treatment. Yet, the mechanism behind such beneficial effects remains unclear. This study constructed a rat model of antibiotic-induced gut dysbiosis to monitor the effects and mechanism of probiotic (Lactobacillus casei Zhang) treatment in maintaining gut homeostasis and restoring the gut microbiota/metabolome. Forty rats were randomly divided into four groups (n = 10 per group): control receiving only saline (Ctrl), antibiotic (AB-Ctrl), antibiotic followed by probiotic (AB-Prob), and antibiotic plus probiotic followed by probiotic (AB + Prob). Rat fecal microbiota and sera were collected at four time points from pre-treatment to post-treatment. The probiotic-treated group (AB + Prob) had significantly more Parabacteroides (P.) goldsteinii after one week of antibiotic and probiotic intervention but fewer antibiotic resistance genes (ARGs)-possessing bacteria (Clostridioides difficile and Burkholderiales bacterium). Consistently, metabolomics data revealed that both probiotic groups had more acetic acid, propionic acid, butyric acid, and valeric acid post treatment. Moreover, a potential probiotic species, P. goldsteinii, strongly correlated with L. casei, as well as propionic acid, butyric acid, and valeric acid. Furthermore, administering probiotic lowered the serum IL-1α level. In contrast, the antibiotic-recipients had a higher irreversible level of IL-1α, suggesting inflammation of the rats. Thus, antibiotic treatment not only led to host gut dysbiosis, but inflammatory responses and an increase in gut ARGs. Daily L. casei Zhang supplementation could alleviate the side effect of cefdinir intervention and facilitate the restoration of gut microbial homeostasis, and these probiotic effects might involve P. goldsteinii-mediated beneficial activities.Guoqiang YaoChenxia CaoMeng ZhangLai-Yu KwokHeping ZhangWenyi ZhangElsevierarticleLactobacillus casei ZhangCefdinirAntibiotic resistance gene (ARG)Parabacteroides goldsteiniiShort-chain fatty acidsIL-1αBiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 5888-5897 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus casei Zhang Cefdinir Antibiotic resistance gene (ARG) Parabacteroides goldsteinii Short-chain fatty acids IL-1α Biotechnology TP248.13-248.65
spellingShingle	Lactobacillus casei Zhang Cefdinir Antibiotic resistance gene (ARG) Parabacteroides goldsteinii Short-chain fatty acids IL-1α Biotechnology TP248.13-248.65 Guoqiang Yao Chenxia Cao Meng Zhang Lai-Yu Kwok Heping Zhang Wenyi Zhang Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
description	Probiotics administration can facilitate the restoration of host gut microbiota/metabolome after antibiotic treatment. Yet, the mechanism behind such beneficial effects remains unclear. This study constructed a rat model of antibiotic-induced gut dysbiosis to monitor the effects and mechanism of probiotic (Lactobacillus casei Zhang) treatment in maintaining gut homeostasis and restoring the gut microbiota/metabolome. Forty rats were randomly divided into four groups (n = 10 per group): control receiving only saline (Ctrl), antibiotic (AB-Ctrl), antibiotic followed by probiotic (AB-Prob), and antibiotic plus probiotic followed by probiotic (AB + Prob). Rat fecal microbiota and sera were collected at four time points from pre-treatment to post-treatment. The probiotic-treated group (AB + Prob) had significantly more Parabacteroides (P.) goldsteinii after one week of antibiotic and probiotic intervention but fewer antibiotic resistance genes (ARGs)-possessing bacteria (Clostridioides difficile and Burkholderiales bacterium). Consistently, metabolomics data revealed that both probiotic groups had more acetic acid, propionic acid, butyric acid, and valeric acid post treatment. Moreover, a potential probiotic species, P. goldsteinii, strongly correlated with L. casei, as well as propionic acid, butyric acid, and valeric acid. Furthermore, administering probiotic lowered the serum IL-1α level. In contrast, the antibiotic-recipients had a higher irreversible level of IL-1α, suggesting inflammation of the rats. Thus, antibiotic treatment not only led to host gut dysbiosis, but inflammatory responses and an increase in gut ARGs. Daily L. casei Zhang supplementation could alleviate the side effect of cefdinir intervention and facilitate the restoration of gut microbial homeostasis, and these probiotic effects might involve P. goldsteinii-mediated beneficial activities.
format	article
author	Guoqiang Yao Chenxia Cao Meng Zhang Lai-Yu Kwok Heping Zhang Wenyi Zhang
author_facet	Guoqiang Yao Chenxia Cao Meng Zhang Lai-Yu Kwok Heping Zhang Wenyi Zhang
author_sort	Guoqiang Yao
title	Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
title_short	Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
title_full	Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
title_fullStr	Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
title_full_unstemmed	Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats
title_sort	lactobacillus casei zhang exerts probiotic effects to antibiotic-treated rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2258c5f07b3c47dfbd17ffe11b6b76c9
work_keys_str_mv	AT guoqiangyao lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats AT chenxiacao lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats AT mengzhang lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats AT laiyukwok lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats AT hepingzhang lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats AT wenyizhang lactobacilluscaseizhangexertsprobioticeffectstoantibiotictreatedrats
_version_	1718445259301584896

Lactobacillus casei Zhang exerts probiotic effects to antibiotic-treated rats

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