Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Abstract Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue m...

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Autores principales:	Yadan Zhang, Ben J. Morgan, Rachel Smith, Christopher R. Fellows, Catherine Thornton, Martyn Snow, Lewis W. Francis, Ilyas M. Khan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/225b7d4c8d184455925e20069ae0a0cc
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spelling	oai:doaj.org-article:225b7d4c8d184455925e20069ae0a0cc2021-12-02T15:06:22ZPlatelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation10.1038/s41598-017-02297-92045-2322https://doaj.org/article/225b7d4c8d184455925e20069ae0a0cc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02297-9https://doaj.org/toc/2045-2322Abstract Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin-1 upregulated. PRP increased cartilage surface cell density 1.5-fold (P < 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5-fold increase in stiffness correlating with a 10-fold upregulation of lysyl oxidase like-1 gene expression (P < 0.001). These data show PRP induces key aspects of post-natal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.Yadan ZhangBen J. MorganRachel SmithChristopher R. FellowsCatherine ThorntonMartyn SnowLewis W. FrancisIlyas M. KhanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yadan Zhang Ben J. Morgan Rachel Smith Christopher R. Fellows Catherine Thornton Martyn Snow Lewis W. Francis Ilyas M. Khan Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
description	Abstract Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin-1 upregulated. PRP increased cartilage surface cell density 1.5-fold (P < 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5-fold increase in stiffness correlating with a 10-fold upregulation of lysyl oxidase like-1 gene expression (P < 0.001). These data show PRP induces key aspects of post-natal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.
format	article
author	Yadan Zhang Ben J. Morgan Rachel Smith Christopher R. Fellows Catherine Thornton Martyn Snow Lewis W. Francis Ilyas M. Khan
author_facet	Yadan Zhang Ben J. Morgan Rachel Smith Christopher R. Fellows Catherine Thornton Martyn Snow Lewis W. Francis Ilyas M. Khan
author_sort	Yadan Zhang
title	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_short	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_fullStr	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full_unstemmed	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_sort	platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with loxl1 activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/225b7d4c8d184455925e20069ae0a0cc
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Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

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