Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica

During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance...

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Autores principales:	Kolathuru Puttamadaiah Ramesha, Nagabhushana Chandra Mohana, Siddaiah Chandra Nayaka, Sreedharamurthy Satish
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	endophyte epigenetic modification Nigrospora sphaerica HDAC DNMT Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/22694785b3844c20b0786e3115679c9d
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id	oai:doaj.org-article:22694785b3844c20b0786e3115679c9d
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spelling	oai:doaj.org-article:22694785b3844c20b0786e3115679c9d2021-12-02T11:40:35ZEpigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica1664-302X10.3389/fmicb.2021.730355https://doaj.org/article/22694785b3844c20b0786e3115679c9d2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.730355/fullhttps://doaj.org/toc/1664-302XDuring plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GCMS) analysis were used to determine secondary metabolite profiling. Epigenetic modifiers like DNA Methyltransferase (DNMT) and Histone deacetylase (HDAC) inhibitors increased the expression of biosynthetic pathways. The activation of new metabolites was observed as a result of the activation of cryptic biosynthetic gene clusters, as well as the silencing of phomalactone in some treatments. When compared to DNMT treatments, HDAC treatments showed a significant increase in cryptic metabolite induction. The induction of cryptic metabolites with biological significance by HDAC treatment is supported by our findings.Kolathuru Puttamadaiah RameshaNagabhushana Chandra MohanaSiddaiah Chandra NayakaSreedharamurthy SatishFrontiers Media S.A.articleendophyteepigenetic modificationNigrospora sphaericaHDACDNMTMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endophyte epigenetic modification Nigrospora sphaerica HDAC DNMT Microbiology QR1-502
spellingShingle	endophyte epigenetic modification Nigrospora sphaerica HDAC DNMT Microbiology QR1-502 Kolathuru Puttamadaiah Ramesha Nagabhushana Chandra Mohana Siddaiah Chandra Nayaka Sreedharamurthy Satish Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
description	During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GCMS) analysis were used to determine secondary metabolite profiling. Epigenetic modifiers like DNA Methyltransferase (DNMT) and Histone deacetylase (HDAC) inhibitors increased the expression of biosynthetic pathways. The activation of new metabolites was observed as a result of the activation of cryptic biosynthetic gene clusters, as well as the silencing of phomalactone in some treatments. When compared to DNMT treatments, HDAC treatments showed a significant increase in cryptic metabolite induction. The induction of cryptic metabolites with biological significance by HDAC treatment is supported by our findings.
format	article
author	Kolathuru Puttamadaiah Ramesha Nagabhushana Chandra Mohana Siddaiah Chandra Nayaka Sreedharamurthy Satish
author_facet	Kolathuru Puttamadaiah Ramesha Nagabhushana Chandra Mohana Siddaiah Chandra Nayaka Sreedharamurthy Satish
author_sort	Kolathuru Puttamadaiah Ramesha
title	Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
title_short	Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
title_full	Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
title_fullStr	Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
title_full_unstemmed	Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica
title_sort	epigenetic modifiers revamp secondary metabolite production in endophytic nigrospora sphaerica
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/22694785b3844c20b0786e3115679c9d
work_keys_str_mv	AT kolathuruputtamadaiahramesha epigeneticmodifiersrevampsecondarymetaboliteproductioninendophyticnigrosporasphaerica AT nagabhushanachandramohana epigeneticmodifiersrevampsecondarymetaboliteproductioninendophyticnigrosporasphaerica AT siddaiahchandranayaka epigeneticmodifiersrevampsecondarymetaboliteproductioninendophyticnigrosporasphaerica AT sreedharamurthysatish epigeneticmodifiersrevampsecondarymetaboliteproductioninendophyticnigrosporasphaerica
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Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica

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