Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome

Guanqun Chao,1 Zhaojun Wang,2 Shuo Zhang3 1Department of General Practice, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, People’s Republic of China; 2The First Clinical Medical College of Zhejiang Chinese Medicine University, Hangzhou, People’s Republic of China; 3Dep...

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Autores principales:	Chao G, Wang Z, Zhang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	irritable bowel syndrome diarrhea neuropeptide protease-activated receptor 2 mast cell Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2271699e31b04b869aa1eb5154ee1761
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spelling	oai:doaj.org-article:2271699e31b04b869aa1eb5154ee17612021-12-02T14:39:09ZResearch on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome1178-7031https://doaj.org/article/2271699e31b04b869aa1eb5154ee17612021-04-01T00:00:00Zhttps://www.dovepress.com/research-on-correlation-between-psychological-factors-mast-cells-and-p-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Guanqun Chao,1 Zhaojun Wang,2 Shuo Zhang3 1Department of General Practice, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, People’s Republic of China; 2The First Clinical Medical College of Zhejiang Chinese Medicine University, Hangzhou, People’s Republic of China; 3Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of ChinaCorrespondence: Shuo ZhangDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of ChinaTel +86 13957192066Email zhangshuotcm@163.comPurpose: The study aimed to explore the level of psychological stress factors, mast cell (MC), and neuropeptide in the occurrence of irritable bowel syndrome (IBS) and the correlation among them, and to identify representative and effective indicators for the pathogenesis and clinical medication development of IBS.Subjects and Methods: Twenty-eight patients (20– 64 years old) with diarrhea-predominant IBS (IBS-D) were included in the IBS-D group and 8 healthy subjects (35– 63 years old) were enrolled in the control group. All subjects accepted the colonoscopic biopsies, self-rating depression scale (SDS) and self-rating anxiety scale (SAS) assessment. MC degranulation, neuropeptide S (NPS), neuropeptide Y (NPY), NPY receptor 2 (NPY2R) and Protease-activated receptor 2 (PAR-2) in colon tissues were performed by Strept Avidin-Biot complex (SABC) immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) detection was used to test the expression of NPS and NPY in peripheral blood plasma and colon tissues. Western blot was applied to examine the level of NPY2R and PAR-2.Results: The level of anxiety and depression of patients with IBS-D was more serious than that in the control. The expression of NPS, NPY and NPY2R was down-regulated in the IBS-D. The total MC and tryptase-positive MC increased significantly in the colon tissue of IBS-D and the expression level of PAR-2 was significantly up-regulated.Conclusion: There has been a close connection among those indicators that the activated MC may up-regulate the function of PAR-2, resulting in the change of neuropeptide (NPS and NPY), successively leading to clinical symptoms and psychological negative changes in the IBS.Keywords: Irritable bowel syndrome, diarrhea, neuropeptide, protease-activated receptor 2, mast cellChao GWang ZZhang SDove Medical Pressarticleirritable bowel syndromediarrheaneuropeptideprotease-activated receptor 2mast cellPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1427-1436 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	irritable bowel syndrome diarrhea neuropeptide protease-activated receptor 2 mast cell Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	irritable bowel syndrome diarrhea neuropeptide protease-activated receptor 2 mast cell Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Chao G Wang Z Zhang S Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
description	Guanqun Chao,1 Zhaojun Wang,2 Shuo Zhang3 1Department of General Practice, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, People’s Republic of China; 2The First Clinical Medical College of Zhejiang Chinese Medicine University, Hangzhou, People’s Republic of China; 3Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of ChinaCorrespondence: Shuo ZhangDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of ChinaTel +86 13957192066Email zhangshuotcm@163.comPurpose: The study aimed to explore the level of psychological stress factors, mast cell (MC), and neuropeptide in the occurrence of irritable bowel syndrome (IBS) and the correlation among them, and to identify representative and effective indicators for the pathogenesis and clinical medication development of IBS.Subjects and Methods: Twenty-eight patients (20– 64 years old) with diarrhea-predominant IBS (IBS-D) were included in the IBS-D group and 8 healthy subjects (35– 63 years old) were enrolled in the control group. All subjects accepted the colonoscopic biopsies, self-rating depression scale (SDS) and self-rating anxiety scale (SAS) assessment. MC degranulation, neuropeptide S (NPS), neuropeptide Y (NPY), NPY receptor 2 (NPY2R) and Protease-activated receptor 2 (PAR-2) in colon tissues were performed by Strept Avidin-Biot complex (SABC) immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) detection was used to test the expression of NPS and NPY in peripheral blood plasma and colon tissues. Western blot was applied to examine the level of NPY2R and PAR-2.Results: The level of anxiety and depression of patients with IBS-D was more serious than that in the control. The expression of NPS, NPY and NPY2R was down-regulated in the IBS-D. The total MC and tryptase-positive MC increased significantly in the colon tissue of IBS-D and the expression level of PAR-2 was significantly up-regulated.Conclusion: There has been a close connection among those indicators that the activated MC may up-regulate the function of PAR-2, resulting in the change of neuropeptide (NPS and NPY), successively leading to clinical symptoms and psychological negative changes in the IBS.Keywords: Irritable bowel syndrome, diarrhea, neuropeptide, protease-activated receptor 2, mast cell
format	article
author	Chao G Wang Z Zhang S
author_facet	Chao G Wang Z Zhang S
author_sort	Chao G
title	Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
title_short	Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
title_full	Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
title_fullStr	Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
title_full_unstemmed	Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome
title_sort	research on correlation between psychological factors, mast cells, and par-2 signal pathway in irritable bowel syndrome
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2271699e31b04b869aa1eb5154ee1761
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Research on Correlation Between Psychological Factors, Mast Cells, and PAR-2 Signal Pathway in Irritable Bowel syndrome

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