MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals

Insulin resistance (IR) is one of the most common metabolic disorders worldwide and is involved in the development of diseases, such as diabetes and cardiovascular diseases, affecting civilisations. The possibility of understanding the molecular mechanism and searching for new biomarkers useful in a...

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Autores principales:	Katarzyna Pastusiak, Eliza Matuszewska, Dagmara Pietkiewicz, Jan Matysiak, Pawel Bogdanski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	insulin resistance normal-weight obesity MALDI protein-peptide profiling Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/2277a22fe06645d8b253a8b471ec5580
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spelling	oai:doaj.org-article:2277a22fe06645d8b253a8b471ec55802021-11-25T18:34:38ZMALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals10.3390/nu131138532072-6643https://doaj.org/article/2277a22fe06645d8b253a8b471ec55802021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3853https://doaj.org/toc/2072-6643Insulin resistance (IR) is one of the most common metabolic disorders worldwide and is involved in the development of diseases, such as diabetes and cardiovascular diseases, affecting civilisations. The possibility of understanding the molecular mechanism and searching for new biomarkers useful in assessing IR can be achieved through modern research techniques such as proteomics. This study assessed the protein–peptide profile among normal-weight patients with IR to understand the mechanisms and to define new risk biomarkers. The research involved 21 IR and 43 healthy, normal-weight individuals, aged 19–65. Serum proteomic patterns were obtained using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The proposed methodology identified six proteins differentiating normal weight IR and insulin sensitive individuals. They were fibrinogen alpha chain, serum albumin, kininogen-1, complement C3, serotransferrin, and Ig gamma-1 chain, which could potentially be related to inflammation. However, further investigation is required to confirm their correlation with IR.Katarzyna PastusiakEliza MatuszewskaDagmara PietkiewiczJan MatysiakPawel BogdanskiMDPI AGarticleinsulin resistancenormal-weight obesityMALDIprotein-peptide profilingNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3853, p 3853 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	insulin resistance normal-weight obesity MALDI protein-peptide profiling Nutrition. Foods and food supply TX341-641
spellingShingle	insulin resistance normal-weight obesity MALDI protein-peptide profiling Nutrition. Foods and food supply TX341-641 Katarzyna Pastusiak Eliza Matuszewska Dagmara Pietkiewicz Jan Matysiak Pawel Bogdanski MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
description	Insulin resistance (IR) is one of the most common metabolic disorders worldwide and is involved in the development of diseases, such as diabetes and cardiovascular diseases, affecting civilisations. The possibility of understanding the molecular mechanism and searching for new biomarkers useful in assessing IR can be achieved through modern research techniques such as proteomics. This study assessed the protein–peptide profile among normal-weight patients with IR to understand the mechanisms and to define new risk biomarkers. The research involved 21 IR and 43 healthy, normal-weight individuals, aged 19–65. Serum proteomic patterns were obtained using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The proposed methodology identified six proteins differentiating normal weight IR and insulin sensitive individuals. They were fibrinogen alpha chain, serum albumin, kininogen-1, complement C3, serotransferrin, and Ig gamma-1 chain, which could potentially be related to inflammation. However, further investigation is required to confirm their correlation with IR.
format	article
author	Katarzyna Pastusiak Eliza Matuszewska Dagmara Pietkiewicz Jan Matysiak Pawel Bogdanski
author_facet	Katarzyna Pastusiak Eliza Matuszewska Dagmara Pietkiewicz Jan Matysiak Pawel Bogdanski
author_sort	Katarzyna Pastusiak
title	MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
title_short	MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
title_full	MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
title_fullStr	MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
title_full_unstemmed	MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals
title_sort	maldi-tof ms characterisation of the serum proteomic profile in insulin-resistant normal-weight individuals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2277a22fe06645d8b253a8b471ec5580
work_keys_str_mv	AT katarzynapastusiak malditofmscharacterisationoftheserumproteomicprofileininsulinresistantnormalweightindividuals AT elizamatuszewska malditofmscharacterisationoftheserumproteomicprofileininsulinresistantnormalweightindividuals AT dagmarapietkiewicz malditofmscharacterisationoftheserumproteomicprofileininsulinresistantnormalweightindividuals AT janmatysiak malditofmscharacterisationoftheserumproteomicprofileininsulinresistantnormalweightindividuals AT pawelbogdanski malditofmscharacterisationoftheserumproteomicprofileininsulinresistantnormalweightindividuals
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MALDI-TOF MS Characterisation of the Serum Proteomic Profile in Insulin-Resistant Normal-Weight Individuals

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