Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers

ABSTRACT: As an important olive component, hydroxytyrosol (HT) has good medicinal and health effects. However, its importance in alleviating immune suppression in broilers has not been established. Therefore, we aimed at evaluating the immunomodulatory and antioxidant effects of HT in immune suppres...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chunlan Shan, Fujun Miao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	hydroxytyrosol cyclophosphamide broilers intestinal immunity antioxidant Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/2277ce6b90124e169945cdb322c37dcd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2277ce6b90124e169945cdb322c37dcd
record_format	dspace
spelling	oai:doaj.org-article:2277ce6b90124e169945cdb322c37dcd2021-11-16T04:09:01ZImmunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers0032-579110.1016/j.psj.2021.101516https://doaj.org/article/2277ce6b90124e169945cdb322c37dcd2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005381https://doaj.org/toc/0032-5791ABSTRACT: As an important olive component, hydroxytyrosol (HT) has good medicinal and health effects. However, its importance in alleviating immune suppression in broilers has not been established. Therefore, we aimed at evaluating the immunomodulatory and antioxidant effects of HT in immune suppressed broilers. Immune suppressed broiler models were established via intraperitoneal injection of 80 mg/kg cyclophosphamide (Cy). Thirty two Cobb 500 male broilers were randomly allocated into 4 groups of 8 each. Broilers in the model (Cy) and HT treatment (Cy+HT) groups were intraperitoneally administered with Cy (80 mg/kg BW) once a day for 3 d. From the 4th d, broilers in the Cy+HT and HT groups were treated with 0.5 mL of 200 mg/L HT solution, once a day, for 7 d. The Cy and Con groups were orally administered with normal saline. On the 14th and 28th d, serum and duodenal samples were obtained for testing. It was found that HT increased villi height (VH)/crypt depth (CD) ratio in the duodenum and suppressed serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels. Moreover, it elevated the expressions of CD4+ and CD8+ T lymphocytes. HT upregulated the mRNA expression levels of interleukin-2 (IL-2), interleukin-4 (IL-4), and interleukin-10 (IL-10), enhanced the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and downregulated malondialdehyde (MDA) levels in Cy-induced immune-suppressed broilers. In conclusion, HT can alleviate immune-suppression as well as enhance immunity and antioxidant activities in the local mucosa of small intestines in broilers. Therefore, it can be used as an immune stimulant. More studies should be performed to confirm our findings and to elucidate on the mechanisms of HT.Chunlan ShanFujun MiaoElsevierarticlehydroxytyrosolcyclophosphamidebroilersintestinal immunityantioxidantAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101516- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydroxytyrosol cyclophosphamide broilers intestinal immunity antioxidant Animal culture SF1-1100
spellingShingle	hydroxytyrosol cyclophosphamide broilers intestinal immunity antioxidant Animal culture SF1-1100 Chunlan Shan Fujun Miao Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
description	ABSTRACT: As an important olive component, hydroxytyrosol (HT) has good medicinal and health effects. However, its importance in alleviating immune suppression in broilers has not been established. Therefore, we aimed at evaluating the immunomodulatory and antioxidant effects of HT in immune suppressed broilers. Immune suppressed broiler models were established via intraperitoneal injection of 80 mg/kg cyclophosphamide (Cy). Thirty two Cobb 500 male broilers were randomly allocated into 4 groups of 8 each. Broilers in the model (Cy) and HT treatment (Cy+HT) groups were intraperitoneally administered with Cy (80 mg/kg BW) once a day for 3 d. From the 4th d, broilers in the Cy+HT and HT groups were treated with 0.5 mL of 200 mg/L HT solution, once a day, for 7 d. The Cy and Con groups were orally administered with normal saline. On the 14th and 28th d, serum and duodenal samples were obtained for testing. It was found that HT increased villi height (VH)/crypt depth (CD) ratio in the duodenum and suppressed serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels. Moreover, it elevated the expressions of CD4+ and CD8+ T lymphocytes. HT upregulated the mRNA expression levels of interleukin-2 (IL-2), interleukin-4 (IL-4), and interleukin-10 (IL-10), enhanced the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and downregulated malondialdehyde (MDA) levels in Cy-induced immune-suppressed broilers. In conclusion, HT can alleviate immune-suppression as well as enhance immunity and antioxidant activities in the local mucosa of small intestines in broilers. Therefore, it can be used as an immune stimulant. More studies should be performed to confirm our findings and to elucidate on the mechanisms of HT.
format	article
author	Chunlan Shan Fujun Miao
author_facet	Chunlan Shan Fujun Miao
author_sort	Chunlan Shan
title	Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
title_short	Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
title_full	Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
title_fullStr	Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
title_full_unstemmed	Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
title_sort	immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/2277ce6b90124e169945cdb322c37dcd
work_keys_str_mv	AT chunlanshan immunomodulatoryandantioxidanteffectsofhydroxytyrosolincyclophosphamideinducedimmunosuppressedbroilers AT fujunmiao immunomodulatoryandantioxidanteffectsofhydroxytyrosolincyclophosphamideinducedimmunosuppressedbroilers
_version_	1718426770703646720

Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers

Ejemplares similares