The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis

Abstract An excessive intestinal inflammatory response may have a role in the pathogenesis of necrotizing enterocolitis (NEC) in very preterm infants. Indole-3-lactic acid (ILA) of breastmilk tryptophan was identified as the anti-inflammatory metabolite involved in probiotic conditioned media from B...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wuyang Huang, Ky Young Cho, Di Meng, W. Allan Walker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/227cfe50da004e89afa80196bad7575e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:227cfe50da004e89afa80196bad7575e
record_format	dspace
spelling	oai:doaj.org-article:227cfe50da004e89afa80196bad7575e2021-12-02T15:51:15ZThe impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis10.1038/s41598-021-87353-12045-2322https://doaj.org/article/227cfe50da004e89afa80196bad7575e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87353-1https://doaj.org/toc/2045-2322Abstract An excessive intestinal inflammatory response may have a role in the pathogenesis of necrotizing enterocolitis (NEC) in very preterm infants. Indole-3-lactic acid (ILA) of breastmilk tryptophan was identified as the anti-inflammatory metabolite involved in probiotic conditioned media from Bifidobacteria longum subsp infantis. This study aimed to explore the molecular endocytic pathways involved in the protective ILA effect against inflammation. H4 cells, Caco-2 cells, C57BL/6 pup and adult mice were used to compare the anti-inflammatory mechanisms between immature and mature enterocytes in vitro and in vivo. The results show that ILA has pleiotropic protective effects on immature enterocytes including anti-inflammatory, anti-viral, and developmental regulatory potentials in a region-dependent and an age-dependent manner. Quantitative transcriptomic analysis revealed a new mechanistic model in which STAT1 pathways play an important role in IL-1β-induced inflammation and ILA has a regulatory effect on STAT1 pathways. These studies were validated by real-time RT-qPCR and STAT1 inhibitor experiments. Different protective reactions of ILA between immature and mature enterocytes indicated that ILA’s effects are developmentally regulated. These findings may be helpful in preventing NEC for premature infants.Wuyang HuangKy Young ChoDi MengW. Allan WalkerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wuyang Huang Ky Young Cho Di Meng W. Allan Walker The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
description	Abstract An excessive intestinal inflammatory response may have a role in the pathogenesis of necrotizing enterocolitis (NEC) in very preterm infants. Indole-3-lactic acid (ILA) of breastmilk tryptophan was identified as the anti-inflammatory metabolite involved in probiotic conditioned media from Bifidobacteria longum subsp infantis. This study aimed to explore the molecular endocytic pathways involved in the protective ILA effect against inflammation. H4 cells, Caco-2 cells, C57BL/6 pup and adult mice were used to compare the anti-inflammatory mechanisms between immature and mature enterocytes in vitro and in vivo. The results show that ILA has pleiotropic protective effects on immature enterocytes including anti-inflammatory, anti-viral, and developmental regulatory potentials in a region-dependent and an age-dependent manner. Quantitative transcriptomic analysis revealed a new mechanistic model in which STAT1 pathways play an important role in IL-1β-induced inflammation and ILA has a regulatory effect on STAT1 pathways. These studies were validated by real-time RT-qPCR and STAT1 inhibitor experiments. Different protective reactions of ILA between immature and mature enterocytes indicated that ILA’s effects are developmentally regulated. These findings may be helpful in preventing NEC for premature infants.
format	article
author	Wuyang Huang Ky Young Cho Di Meng W. Allan Walker
author_facet	Wuyang Huang Ky Young Cho Di Meng W. Allan Walker
author_sort	Wuyang Huang
title	The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
title_short	The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
title_full	The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
title_fullStr	The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
title_full_unstemmed	The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
title_sort	impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/227cfe50da004e89afa80196bad7575e
work_keys_str_mv	AT wuyanghuang theimpactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT kyyoungcho theimpactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT dimeng theimpactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT wallanwalker theimpactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT wuyanghuang impactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT kyyoungcho impactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT dimeng impactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis AT wallanwalker impactofindole3lacticacidonimmatureintestinalinnateimmunityanddevelopmentatranscriptomicanalysis
_version_	1718385618713575424

The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis

Ejemplares similares