Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis

T-regulatory (Treg)/T-helper 17 (Th17) imbalance is associated with preeclampsia (PE). Herein, we aimed to explore the effect and mechanism of lncRNA NEAT1 on the Treg/Th17 balance. The levels of nuclear enriched abundant transcript 1 (NEAT1), miR-485-5p, and absent in melanoma 2 (AIM2) in CD4+ T ce...

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Autores principales:	Jiying Chen, Yonggang Zhang, Wenqing Tan, Hanchao Gao, Shuixiu Xiao, Jinhua Gao, Zhiying Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	preeclampsia neat1 lncrna treg/th17 mir-485-5p/aim2 axis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/227e49181af0417fa24c24f8a8292e53
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spelling	oai:doaj.org-article:227e49181af0417fa24c24f8a8292e532021-11-04T15:51:53ZSilencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis2165-59792165-598710.1080/21655979.2021.1982306https://doaj.org/article/227e49181af0417fa24c24f8a8292e532021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982306https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987T-regulatory (Treg)/T-helper 17 (Th17) imbalance is associated with preeclampsia (PE). Herein, we aimed to explore the effect and mechanism of lncRNA NEAT1 on the Treg/Th17 balance. The levels of nuclear enriched abundant transcript 1 (NEAT1), miR-485-5p, and absent in melanoma 2 (AIM2) in CD4+ T cells were determined using real-time quantitative polymerase chain reaction (RT-qPCR). Treg and Th17 cells were examined using flow cytometry. The relationship between miR-485-5p and NEAT1 or AIM2 was assessed using a dual-luciferase reporter assay. Pearson’s correlation coefficient was used to analyze the correlation. All the data indicated that NEAT1 was upregulated in PE. The number of Treg cells decreased and was negatively related to NEAT1, whereas the number of Th17 cells increased and was positively related to NEAT1 in PE. Knockdown of NEAT1 increased the Treg cells and Treg/Th17 but decreased Th17 cells. Furthermore, NEAT1 sponges miR-485-5p to suppress the target AIM2 levels. Inhibition of miR-485-5p or upregulation of AIM2 abrogated the effect on Treg/Th17 balance induced by knockdown of NEAT1. In conclusion, silencing of NEAT1 promoted Treg/Th17 balance via the miR-485-5p/AIM2 axis in PE, suggesting that NEAT1 is a potential target for the treatment of PE.Jiying ChenYonggang ZhangWenqing TanHanchao GaoShuixiu XiaoJinhua GaoZhiying ZhuTaylor & Francis Grouparticlepreeclampsianeat1lncrnatreg/th17mir-485-5p/aim2 axisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8768-8777 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	preeclampsia neat1 lncrna treg/th17 mir-485-5p/aim2 axis Biotechnology TP248.13-248.65
spellingShingle	preeclampsia neat1 lncrna treg/th17 mir-485-5p/aim2 axis Biotechnology TP248.13-248.65 Jiying Chen Yonggang Zhang Wenqing Tan Hanchao Gao Shuixiu Xiao Jinhua Gao Zhiying Zhu Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
description	T-regulatory (Treg)/T-helper 17 (Th17) imbalance is associated with preeclampsia (PE). Herein, we aimed to explore the effect and mechanism of lncRNA NEAT1 on the Treg/Th17 balance. The levels of nuclear enriched abundant transcript 1 (NEAT1), miR-485-5p, and absent in melanoma 2 (AIM2) in CD4+ T cells were determined using real-time quantitative polymerase chain reaction (RT-qPCR). Treg and Th17 cells were examined using flow cytometry. The relationship between miR-485-5p and NEAT1 or AIM2 was assessed using a dual-luciferase reporter assay. Pearson’s correlation coefficient was used to analyze the correlation. All the data indicated that NEAT1 was upregulated in PE. The number of Treg cells decreased and was negatively related to NEAT1, whereas the number of Th17 cells increased and was positively related to NEAT1 in PE. Knockdown of NEAT1 increased the Treg cells and Treg/Th17 but decreased Th17 cells. Furthermore, NEAT1 sponges miR-485-5p to suppress the target AIM2 levels. Inhibition of miR-485-5p or upregulation of AIM2 abrogated the effect on Treg/Th17 balance induced by knockdown of NEAT1. In conclusion, silencing of NEAT1 promoted Treg/Th17 balance via the miR-485-5p/AIM2 axis in PE, suggesting that NEAT1 is a potential target for the treatment of PE.
format	article
author	Jiying Chen Yonggang Zhang Wenqing Tan Hanchao Gao Shuixiu Xiao Jinhua Gao Zhiying Zhu
author_facet	Jiying Chen Yonggang Zhang Wenqing Tan Hanchao Gao Shuixiu Xiao Jinhua Gao Zhiying Zhu
author_sort	Jiying Chen
title	Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
title_short	Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
title_full	Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
title_fullStr	Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
title_full_unstemmed	Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis
title_sort	silencing of long non-coding rna neat1 improves treg/th17 imbalance in preeclampsia via the mir-485-5p/aim2 axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/227e49181af0417fa24c24f8a8292e53
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Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis

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