Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells

Xinying Wu1, Yanbin Tan1, Hui Mao2, Minming Zhang11Department of Radiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; 2Department of Radiology, Center for Systems Imaging, Emory University School of Medicine, Atlanta, Georgia, USAAbstract: Iron ox...

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Autores principales:	Xinying Wu, Yanbin Tan, Hui Mao, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/228237fee588499c905ed2f09ff5ca23
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spelling	oai:doaj.org-article:228237fee588499c905ed2f09ff5ca232021-12-02T02:59:24ZToxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells1176-91141178-2013https://doaj.org/article/228237fee588499c905ed2f09ff5ca232010-05-01T00:00:00Zhttp://www.dovepress.com/toxic-effects-of-iron-oxide-nanoparticles-on-human-umbilical-vein-endo-a4513https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xinying Wu1, Yanbin Tan1, Hui Mao2, Minming Zhang11Department of Radiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; 2Department of Radiology, Center for Systems Imaging, Emory University School of Medicine, Atlanta, Georgia, USAAbstract: Iron oxide nanoparticles (IONPs) have been employed for hyperthermia treatments, stem cell therapies, cell labeling, and imaging modalities. The biocompatibility and cytotoxic effects of iron oxide nanoparticles when used in biomedical applications, however, are an ongoing concern. Endothelial cells have a critical role in this research dealing with tumors, cardiovascular disease and inflammation. However, there is little information dealing with the biologic effects of IONPs on the endothelial cell. This paper deals with the influence of dextran and citric acid coated IONPs on the behavior and function of human umbilical vein endothelial cells (HUVECs). After exposing endothelial cells to IONPs, dose-dependent effects on HUVECs viability, cytoskeleton and function were determined. Both citric acid and dextran coated particles appeared to be largely internalized by HUVECs through endocytosis and contribute to eventual cell death possibly by apoptosis. Cytoskeletal structures were greatly disrupted, as evidenced by diminished vinculin spots, and disorganized actin fiber and tubulin networks. The capacity of HUVECs to form a vascular network on Matrigel&trade; diminished after exposure to IONPs. Cell migration/invasion were inhibited significantly even at very low iron concentrations (0.1 mM). The results of this study indicate the great importance of thoroughly understanding nanoparticle-cell interactions, and the potential to exploit this understanding in tumor therapy applications involving IONPs as thermo/chemoembolization agents.Keywords: iron oxide nanoparticles, cytotoxicity, in vitro test, cytoskeleton, human umbilical vein endothelial cell Xinying WuYanbin TanHui Maoet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 385-399 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xinying Wu Yanbin Tan Hui Mao et al Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
description	Xinying Wu1, Yanbin Tan1, Hui Mao2, Minming Zhang11Department of Radiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; 2Department of Radiology, Center for Systems Imaging, Emory University School of Medicine, Atlanta, Georgia, USAAbstract: Iron oxide nanoparticles (IONPs) have been employed for hyperthermia treatments, stem cell therapies, cell labeling, and imaging modalities. The biocompatibility and cytotoxic effects of iron oxide nanoparticles when used in biomedical applications, however, are an ongoing concern. Endothelial cells have a critical role in this research dealing with tumors, cardiovascular disease and inflammation. However, there is little information dealing with the biologic effects of IONPs on the endothelial cell. This paper deals with the influence of dextran and citric acid coated IONPs on the behavior and function of human umbilical vein endothelial cells (HUVECs). After exposing endothelial cells to IONPs, dose-dependent effects on HUVECs viability, cytoskeleton and function were determined. Both citric acid and dextran coated particles appeared to be largely internalized by HUVECs through endocytosis and contribute to eventual cell death possibly by apoptosis. Cytoskeletal structures were greatly disrupted, as evidenced by diminished vinculin spots, and disorganized actin fiber and tubulin networks. The capacity of HUVECs to form a vascular network on Matrigel&trade; diminished after exposure to IONPs. Cell migration/invasion were inhibited significantly even at very low iron concentrations (0.1 mM). The results of this study indicate the great importance of thoroughly understanding nanoparticle-cell interactions, and the potential to exploit this understanding in tumor therapy applications involving IONPs as thermo/chemoembolization agents.Keywords: iron oxide nanoparticles, cytotoxicity, in vitro test, cytoskeleton, human umbilical vein endothelial cell
format	article
author	Xinying Wu Yanbin Tan Hui Mao et al
author_facet	Xinying Wu Yanbin Tan Hui Mao et al
author_sort	Xinying Wu
title	Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
title_short	Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
title_full	Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
title_fullStr	Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
title_full_unstemmed	Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
title_sort	toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/228237fee588499c905ed2f09ff5ca23
work_keys_str_mv	AT xinyingwu toxiceffectsofironoxidenanoparticlesonhumanumbilicalveinendothelialcells AT yanbintan toxiceffectsofironoxidenanoparticlesonhumanumbilicalveinendothelialcells AT huimao toxiceffectsofironoxidenanoparticlesonhumanumbilicalveinendothelialcells AT etal toxiceffectsofironoxidenanoparticlesonhumanumbilicalveinendothelialcells
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Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells

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