Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)

Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Centella asiatica has be...

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Autores principales:	Visarut Buranasudja, Dolly Rani, Ashwini Malla, Khwanlada Kobtrakul, Sornkanok Vimolmangkang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2283891f8e344e0b9c3419b7a9a7d71f
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spelling	oai:doaj.org-article:2283891f8e344e0b9c3419b7a9a7d71f2021-12-02T14:34:03ZInsights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)10.1038/s41598-021-92958-72045-2322https://doaj.org/article/2283891f8e344e0b9c3419b7a9a7d71f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92958-7https://doaj.org/toc/2045-2322Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Centella asiatica has been used to treat multitude of diseases for centuries. Previous investigations demonstrated that extracts from C. asiatica have a broad range of beneficial activities through their antioxidant activity. Hence, the extract from this medicinal plant could be a great candidate for anti-skin-aging agent. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Information from HPTLC-DPPH and HPLC analysis suggested that the callus extract comprises distinctive antioxidant compounds, compared with those isolated from authentic plant. Moreover, results from cell culture experiment demonstrated that callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.Visarut BuranasudjaDolly RaniAshwini MallaKhwanlada KobtrakulSornkanok VimolmangkangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Visarut Buranasudja Dolly Rani Ashwini Malla Khwanlada Kobtrakul Sornkanok Vimolmangkang Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
description	Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Centella asiatica has been used to treat multitude of diseases for centuries. Previous investigations demonstrated that extracts from C. asiatica have a broad range of beneficial activities through their antioxidant activity. Hence, the extract from this medicinal plant could be a great candidate for anti-skin-aging agent. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Information from HPTLC-DPPH and HPLC analysis suggested that the callus extract comprises distinctive antioxidant compounds, compared with those isolated from authentic plant. Moreover, results from cell culture experiment demonstrated that callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.
format	article
author	Visarut Buranasudja Dolly Rani Ashwini Malla Khwanlada Kobtrakul Sornkanok Vimolmangkang
author_facet	Visarut Buranasudja Dolly Rani Ashwini Malla Khwanlada Kobtrakul Sornkanok Vimolmangkang
author_sort	Visarut Buranasudja
title	Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
title_short	Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
title_full	Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
title_fullStr	Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
title_full_unstemmed	Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)
title_sort	insights into antioxidant activities and anti-skin-aging potential of callus extract from centella asiatica (l.)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2283891f8e344e0b9c3419b7a9a7d71f
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Insights into antioxidant activities and anti-skin-aging potential of callus extract from Centella asiatica (L.)

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