Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.

Human metapneumovirus (HMPV) encodes a small hydrophobic (SH) protein of unknown function. HMPV from which the SH open reading frame was deleted (HMPVΔSH) was viable and displayed similar replication kinetics, cytopathic effect and plaque size compared with wild type HMPV in several cell-lines. In a...

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Autores principales:	Miranda de Graaf, Sander Herfst, Jamil Aarbiou, Peter C Burgers, Fatiha Zaaraoui-Boutahar, Maarten Bijl, Wilfred van Ijcken, Eefje J A Schrauwen, Albert D M E Osterhaus, Theo M Luider, Bob J Scholte, Ron A M Fouchier, Arno C Andeweg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/228aa1f0d2004a7998ff55e0cca15a17
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spelling	oai:doaj.org-article:228aa1f0d2004a7998ff55e0cca15a172021-11-18T07:54:32ZSmall hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.1932-620310.1371/journal.pone.0058572https://doaj.org/article/228aa1f0d2004a7998ff55e0cca15a172013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23484037/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human metapneumovirus (HMPV) encodes a small hydrophobic (SH) protein of unknown function. HMPV from which the SH open reading frame was deleted (HMPVΔSH) was viable and displayed similar replication kinetics, cytopathic effect and plaque size compared with wild type HMPV in several cell-lines. In addition, no differences were observed in infection efficiency or cell-to-cell spreading in human primary bronchial epithelial cells (HPBEC) cultured at an air-liquid interphase. Host gene expression was analyzed in A549 cells infected with HMPV or HMPVΔSH using microarrays and mass spectrometry (MS) based techniques at multiple time points post infection. Only minor differences were observed in mRNA or protein expression levels. A possible function of HMPV SH as apoptosis blocker, as proposed for several members of the family Paramyxoviridae, was rejected based on this analysis. So far, a clear phenotype of HMPV SH deletion mutants in vitro at the virus and host levels is absent.Miranda de GraafSander HerfstJamil AarbiouPeter C BurgersFatiha Zaaraoui-BoutaharMaarten BijlWilfred van IjckenEefje J A SchrauwenAlbert D M E OsterhausTheo M LuiderBob J ScholteRon A M FouchierArno C AndewegPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58572 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miranda de Graaf Sander Herfst Jamil Aarbiou Peter C Burgers Fatiha Zaaraoui-Boutahar Maarten Bijl Wilfred van Ijcken Eefje J A Schrauwen Albert D M E Osterhaus Theo M Luider Bob J Scholte Ron A M Fouchier Arno C Andeweg Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
description	Human metapneumovirus (HMPV) encodes a small hydrophobic (SH) protein of unknown function. HMPV from which the SH open reading frame was deleted (HMPVΔSH) was viable and displayed similar replication kinetics, cytopathic effect and plaque size compared with wild type HMPV in several cell-lines. In addition, no differences were observed in infection efficiency or cell-to-cell spreading in human primary bronchial epithelial cells (HPBEC) cultured at an air-liquid interphase. Host gene expression was analyzed in A549 cells infected with HMPV or HMPVΔSH using microarrays and mass spectrometry (MS) based techniques at multiple time points post infection. Only minor differences were observed in mRNA or protein expression levels. A possible function of HMPV SH as apoptosis blocker, as proposed for several members of the family Paramyxoviridae, was rejected based on this analysis. So far, a clear phenotype of HMPV SH deletion mutants in vitro at the virus and host levels is absent.
format	article
author	Miranda de Graaf Sander Herfst Jamil Aarbiou Peter C Burgers Fatiha Zaaraoui-Boutahar Maarten Bijl Wilfred van Ijcken Eefje J A Schrauwen Albert D M E Osterhaus Theo M Luider Bob J Scholte Ron A M Fouchier Arno C Andeweg
author_facet	Miranda de Graaf Sander Herfst Jamil Aarbiou Peter C Burgers Fatiha Zaaraoui-Boutahar Maarten Bijl Wilfred van Ijcken Eefje J A Schrauwen Albert D M E Osterhaus Theo M Luider Bob J Scholte Ron A M Fouchier Arno C Andeweg
author_sort	Miranda de Graaf
title	Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
title_short	Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
title_full	Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
title_fullStr	Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
title_full_unstemmed	Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
title_sort	small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/228aa1f0d2004a7998ff55e0cca15a17
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Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro.

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