Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer

Abstract Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of bone conditions like osteoporosis or metastatic bone cancer. Therefore, it is gaining increasing importance in an emerging area of regenerative medicine—regenerative rehabilitatio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lívia Santos, Aslihan Ugun-Klusek, Clare Coveney, David J. Boocock
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/228cbdb7811546228228ea7a661c635c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:228cbdb7811546228228ea7a661c635c
record_format	dspace
spelling	oai:doaj.org-article:228cbdb7811546228228ea7a661c635c2021-12-02T14:58:13ZMultiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer10.1038/s41536-021-00141-32057-3995https://doaj.org/article/228cbdb7811546228228ea7a661c635c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00141-3https://doaj.org/toc/2057-3995Abstract Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of bone conditions like osteoporosis or metastatic bone cancer. Therefore, it is gaining increasing importance in an emerging area of regenerative medicine—regenerative rehabilitation (RR). Osteocytes are mechanosensitive and secretory bone cells that orchestrate bone anabolism and hence postulated to be an attractive target of regenerative exercise interventions. However, the human osteocyte signalling pathways and processes evoked upon exercise remain to be fully identified. Making use of a computer-controlled bioreactor that mimics exercise and the latest omics approaches, RNA sequencing (RNA-seq) and tandem liquid chromatography-mass spectrometry (LC-MS), we mapped the transcriptome and secretome of mechanically stretched human osteocytic cells. We discovered that a single bout of cyclic stretch activated network processes and signalling pathways likely to modulate bone regeneration and cancer. Furthermore, a comparison between the transcriptome and secretome of stretched human and mouse osteocytic cells revealed dissimilar results, despite both species sharing evolutionarily conserved signalling pathways. These findings suggest that osteocytes can be targeted by exercise-driven RR protocols aiming to modulate bone regeneration or metastatic bone cancer.Lívia SantosAslihan Ugun-KlusekClare CoveneyDavid J. BoocockNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Lívia Santos Aslihan Ugun-Klusek Clare Coveney David J. Boocock Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
description	Abstract Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of bone conditions like osteoporosis or metastatic bone cancer. Therefore, it is gaining increasing importance in an emerging area of regenerative medicine—regenerative rehabilitation (RR). Osteocytes are mechanosensitive and secretory bone cells that orchestrate bone anabolism and hence postulated to be an attractive target of regenerative exercise interventions. However, the human osteocyte signalling pathways and processes evoked upon exercise remain to be fully identified. Making use of a computer-controlled bioreactor that mimics exercise and the latest omics approaches, RNA sequencing (RNA-seq) and tandem liquid chromatography-mass spectrometry (LC-MS), we mapped the transcriptome and secretome of mechanically stretched human osteocytic cells. We discovered that a single bout of cyclic stretch activated network processes and signalling pathways likely to modulate bone regeneration and cancer. Furthermore, a comparison between the transcriptome and secretome of stretched human and mouse osteocytic cells revealed dissimilar results, despite both species sharing evolutionarily conserved signalling pathways. These findings suggest that osteocytes can be targeted by exercise-driven RR protocols aiming to modulate bone regeneration or metastatic bone cancer.
format	article
author	Lívia Santos Aslihan Ugun-Klusek Clare Coveney David J. Boocock
author_facet	Lívia Santos Aslihan Ugun-Klusek Clare Coveney David J. Boocock
author_sort	Lívia Santos
title	Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
title_short	Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
title_full	Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
title_fullStr	Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
title_full_unstemmed	Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
title_sort	multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/228cbdb7811546228228ea7a661c635c
work_keys_str_mv	AT liviasantos multiomicanalysisofstretchedosteocytesrevealsprocessesandsignallinglinkedtoboneregenerationandcancer AT aslihanugunklusek multiomicanalysisofstretchedosteocytesrevealsprocessesandsignallinglinkedtoboneregenerationandcancer AT clarecoveney multiomicanalysisofstretchedosteocytesrevealsprocessesandsignallinglinkedtoboneregenerationandcancer AT davidjboocock multiomicanalysisofstretchedosteocytesrevealsprocessesandsignallinglinkedtoboneregenerationandcancer
_version_	1718389312800686080

Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer

Ejemplares similares