A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pa...

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Autores principales:	Paris Roidos, Stephanie Sungalee, Salvatore Benfatto, Özdemirhan Serçin, Adrian M. Stütz, Amir Abdollahi, Jan Mauer, Frank T. Zenke, Jan O. Korbel, Balca R. Mardin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/229117b82514431a854b76cda0b7c54e
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spelling	oai:doaj.org-article:229117b82514431a854b76cda0b7c54e2021-12-02T19:06:29ZA scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice10.1038/s41467-020-17962-32041-1723https://doaj.org/article/229117b82514431a854b76cda0b7c54e2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17962-3https://doaj.org/toc/2041-1723Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pathways.Paris RoidosStephanie SungaleeSalvatore BenfattoÖzdemirhan SerçinAdrian M. StützAmir AbdollahiJan MauerFrank T. ZenkeJan O. KorbelBalca R. MardinNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Paris Roidos Stephanie Sungalee Salvatore Benfatto Özdemirhan Serçin Adrian M. Stütz Amir Abdollahi Jan Mauer Frank T. Zenke Jan O. Korbel Balca R. Mardin A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
description	Cells employ different repair pathways to repair DNA double strand breaks. Here, the authors develop a CRISPR/Cas9-dependent method to study choices in DNA repair called the Color Assay Tracing-Repair (CAT-R) which simultaneously measure outcomes of DSB repair via end-protection and end-resection pathways.
format	article
author	Paris Roidos Stephanie Sungalee Salvatore Benfatto Özdemirhan Serçin Adrian M. Stütz Amir Abdollahi Jan Mauer Frank T. Zenke Jan O. Korbel Balca R. Mardin
author_facet	Paris Roidos Stephanie Sungalee Salvatore Benfatto Özdemirhan Serçin Adrian M. Stütz Amir Abdollahi Jan Mauer Frank T. Zenke Jan O. Korbel Balca R. Mardin
author_sort	Paris Roidos
title	A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_short	A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_full	A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_fullStr	A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_full_unstemmed	A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
title_sort	scalable crispr/cas9-based fluorescent reporter assay to study dna double-strand break repair choice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/229117b82514431a854b76cda0b7c54e
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A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

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