A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane

Marketa Bacakova,*,1 Julia Pajorova,*,1,2 Antonin Broz,1 Daniel Hadraba,1,3 Frantisek Lopot,3 Anna Zavadakova,4 Lucie Vistejnova,4 Milan Beno,5 Ivan Kostic,6 Vera Jencova,7 Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Acade...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bacakova M, Pajorova J, Broz A, Hadraba D, Lopot F, Zavadakova A, Vistejnova L, Beno M, Kostic I, Jencova V, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	full-thickness skin substitutes collagen hydrogel fibroblast and keratinocyte co-cultivation fibrin nanostructure Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/22953eda256543ad8e2f0a2733b38f79
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:22953eda256543ad8e2f0a2733b38f79
record_format	dspace
spelling	oai:doaj.org-article:22953eda256543ad8e2f0a2733b38f792021-12-02T04:14:58ZA two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane1178-2013https://doaj.org/article/22953eda256543ad8e2f0a2733b38f792019-07-01T00:00:00Zhttps://www.dovepress.com/a-two-layer-skin-construct-consisting-of-a-collagen-hydrogel-reinforce-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Marketa Bacakova,,1 Julia Pajorova,,1,2 Antonin Broz,1 Daniel Hadraba,1,3 Frantisek Lopot,3 Anna Zavadakova,4 Lucie Vistejnova,4 Milan Beno,5 Ivan Kostic,6 Vera Jencova,7 Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic; 22nd Faculty of Medicine, Charles University, Prague, Czech Republic; 3Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic; 4Biomedical Center, Medical Faculty in Pilsen, Charles University, Pilsen, Czech Republic; 5Institute of Experimental Endocrinology, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovak Republic; 6Institute of Informatics, Slovak Academy of Sciences, Bratislava, Slovak Republic; 7Department of Chemistry, Technical University of Liberec, Liberec, Czech Republic*These authors contributed equally to this workBackground: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells.Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh.Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer.Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.Keywords: full-thickness skin substitutes, collagen hydrogel, fibroblast and keratinocyte co-cultivation, fibrin, nanostructureBacakova MPajorova JBroz AHadraba DLopot FZavadakova AVistejnova LBeno MKostic IJencova VBacakova LDove Medical Pressarticlefull-thickness skin substitutescollagen hydrogelfibroblast and keratinocyte co-cultivationfibrinnanostructureMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5033-5050 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	full-thickness skin substitutes collagen hydrogel fibroblast and keratinocyte co-cultivation fibrin nanostructure Medicine (General) R5-920
spellingShingle	full-thickness skin substitutes collagen hydrogel fibroblast and keratinocyte co-cultivation fibrin nanostructure Medicine (General) R5-920 Bacakova M Pajorova J Broz A Hadraba D Lopot F Zavadakova A Vistejnova L Beno M Kostic I Jencova V Bacakova L A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
description	Marketa Bacakova,,1 Julia Pajorova,,1,2 Antonin Broz,1 Daniel Hadraba,1,3 Frantisek Lopot,3 Anna Zavadakova,4 Lucie Vistejnova,4 Milan Beno,5 Ivan Kostic,6 Vera Jencova,7 Lucie Bacakova11Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic; 22nd Faculty of Medicine, Charles University, Prague, Czech Republic; 3Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic; 4Biomedical Center, Medical Faculty in Pilsen, Charles University, Pilsen, Czech Republic; 5Institute of Experimental Endocrinology, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovak Republic; 6Institute of Informatics, Slovak Academy of Sciences, Bratislava, Slovak Republic; 7Department of Chemistry, Technical University of Liberec, Liberec, Czech Republic*These authors contributed equally to this workBackground: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells.Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh.Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer.Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.Keywords: full-thickness skin substitutes, collagen hydrogel, fibroblast and keratinocyte co-cultivation, fibrin, nanostructure
format	article
author	Bacakova M Pajorova J Broz A Hadraba D Lopot F Zavadakova A Vistejnova L Beno M Kostic I Jencova V Bacakova L
author_facet	Bacakova M Pajorova J Broz A Hadraba D Lopot F Zavadakova A Vistejnova L Beno M Kostic I Jencova V Bacakova L
author_sort	Bacakova M
title	A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
title_short	A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
title_full	A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
title_fullStr	A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
title_full_unstemmed	A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
title_sort	two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/22953eda256543ad8e2f0a2733b38f79
work_keys_str_mv	AT bacakovam atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT pajorovaj atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT broza atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT hadrabad atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT lopotf atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT zavadakovaa atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT vistejnoval atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT benom atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT kostici atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT jencovav atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT bacakoval atwolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT bacakovam twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT pajorovaj twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT broza twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT hadrabad twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT lopotf twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT zavadakovaa twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT vistejnoval twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT benom twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT kostici twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT jencovav twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane AT bacakoval twolayerskinconstructconsistingofacollagenhydrogelreinforcedbyafibrincoatedpolylactidenanofibrousmembrane
_version_	1718401355062706176

A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane

Ejemplares similares