Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis...
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Scientific Сentre for Family Health and Human Reproduction Problems
2013
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oai:doaj.org-article:229aa0f4b27247949d8557e3db90e1c62021-11-23T06:14:29ZProteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups2541-94202587-9596https://doaj.org/article/229aa0f4b27247949d8557e3db90e1c62013-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1697https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined.S. N. KozlovV. B. NikolaevE. Y. MarkovL. V. MironovaL. Y. UrbanovichScientific Сentre for Family Health and Human Reproduction Problemsarticlevibrio choleraevibrio choleraeproteasessubcellular fractionenzyme-electrophoresisimmunoglobulins gScienceQRUActa Biomedica Scientifica, Vol 0, Iss 6, Pp 145-148 (2013) |
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vibrio cholerae vibrio cholerae proteases subcellular fraction enzyme-electrophoresis immunoglobulins g Science Q |
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vibrio cholerae vibrio cholerae proteases subcellular fraction enzyme-electrophoresis immunoglobulins g Science Q S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
description |
With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined. |
format |
article |
author |
S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich |
author_facet |
S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich |
author_sort |
S. N. Kozlov |
title |
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
title_short |
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
title_full |
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
title_fullStr |
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
title_full_unstemmed |
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
title_sort |
proteolytic cleavage of immunoglobulins g by subcellular fractions of vibrio cholerae o1 and o139 serougroups |
publisher |
Scientific Сentre for Family Health and Human Reproduction Problems |
publishDate |
2013 |
url |
https://doaj.org/article/229aa0f4b27247949d8557e3db90e1c6 |
work_keys_str_mv |
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_version_ |
1718417118940102656 |