Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups

With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis...

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Autores principales: S. N. Kozlov, V. B. Nikolaev, E. Y. Markov, L. V. Mironova, L. Y. Urbanovich
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Lenguaje:RU
Publicado: Scientific Сentre for Family Health and Human Reproduction Problems 2013
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Acceso en línea:https://doaj.org/article/229aa0f4b27247949d8557e3db90e1c6
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spelling oai:doaj.org-article:229aa0f4b27247949d8557e3db90e1c62021-11-23T06:14:29ZProteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups2541-94202587-9596https://doaj.org/article/229aa0f4b27247949d8557e3db90e1c62013-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1697https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined.S. N. KozlovV. B. NikolaevE. Y. MarkovL. V. MironovaL. Y. UrbanovichScientific Сentre for Family Health and Human Reproduction Problemsarticlevibrio choleraevibrio choleraeproteasessubcellular fractionenzyme-electrophoresisimmunoglobulins gScienceQRUActa Biomedica Scientifica, Vol 0, Iss 6, Pp 145-148 (2013)
institution DOAJ
collection DOAJ
language RU
topic vibrio cholerae
vibrio cholerae
proteases
subcellular fraction
enzyme-electrophoresis
immunoglobulins g
Science
Q
spellingShingle vibrio cholerae
vibrio cholerae
proteases
subcellular fraction
enzyme-electrophoresis
immunoglobulins g
Science
Q
S. N. Kozlov
V. B. Nikolaev
E. Y. Markov
L. V. Mironova
L. Y. Urbanovich
Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
description With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined.
format article
author S. N. Kozlov
V. B. Nikolaev
E. Y. Markov
L. V. Mironova
L. Y. Urbanovich
author_facet S. N. Kozlov
V. B. Nikolaev
E. Y. Markov
L. V. Mironova
L. Y. Urbanovich
author_sort S. N. Kozlov
title Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
title_short Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
title_full Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
title_fullStr Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
title_full_unstemmed Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
title_sort proteolytic cleavage of immunoglobulins g by subcellular fractions of vibrio cholerae o1 and o139 serougroups
publisher Scientific Сentre for Family Health and Human Reproduction Problems
publishDate 2013
url https://doaj.org/article/229aa0f4b27247949d8557e3db90e1c6
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AT vbnikolaev proteolyticcleavageofimmunoglobulinsgbysubcellularfractionsofvibriocholeraeo1ando139serougroups
AT eymarkov proteolyticcleavageofimmunoglobulinsgbysubcellularfractionsofvibriocholeraeo1ando139serougroups
AT lvmironova proteolyticcleavageofimmunoglobulinsgbysubcellularfractionsofvibriocholeraeo1ando139serougroups
AT lyurbanovich proteolyticcleavageofimmunoglobulinsgbysubcellularfractionsofvibriocholeraeo1ando139serougroups
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