Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes

Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patie...

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Autores principales:	Weng Ruiqiang, Liu Sudong, Gu Xiaodong, Zhong Zhixiong
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	b cell receptor repertoire repertoire sequencing in-stent restenosis type 2 diabetes mellitus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/229ca2f20cee49eba0a632a97b041eab
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spelling	oai:doaj.org-article:229ca2f20cee49eba0a632a97b041eab2021-12-05T14:10:41ZClonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes2391-541210.1515/biol-2021-0091https://doaj.org/article/229ca2f20cee49eba0a632a97b041eab2021-08-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0091https://doaj.org/toc/2391-5412Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patients with ISR remains unclear. This study aims to profile the BCR repertoire in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire profiles using DNA-seq. Our results showed that the diversity of amino acid sequences in ISR DM patients was higher than that in ISR −DM patients. The frequencies of 21 V/J paired genes differed between ISR DM and −ISR DM patients, while frequencies of 5 V/J paired genes differed between ISR DM and ISR −DM. The −ISR −DM group presented the highest clonotype overlap rate, while ISR DM patients presented the lowest overlap rate. Our study presented the BCR repertoires in patients with ISR/T2DM. The data suggested different BCR signatures between patients with ISR and T2DM. Further analysis of BCR profiles would enhance understanding of ISR.Weng RuiqiangLiu SudongGu XiaodongZhong ZhixiongDe Gruyterarticleb cell receptor repertoirerepertoire sequencingin-stent restenosistype 2 diabetes mellitusBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 884-898 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	b cell receptor repertoire repertoire sequencing in-stent restenosis type 2 diabetes mellitus Biology (General) QH301-705.5
spellingShingle	b cell receptor repertoire repertoire sequencing in-stent restenosis type 2 diabetes mellitus Biology (General) QH301-705.5 Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
description	Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patients with ISR remains unclear. This study aims to profile the BCR repertoire in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire profiles using DNA-seq. Our results showed that the diversity of amino acid sequences in ISR DM patients was higher than that in ISR −DM patients. The frequencies of 21 V/J paired genes differed between ISR DM and −ISR DM patients, while frequencies of 5 V/J paired genes differed between ISR DM and ISR −DM. The −ISR −DM group presented the highest clonotype overlap rate, while ISR DM patients presented the lowest overlap rate. Our study presented the BCR repertoires in patients with ISR/T2DM. The data suggested different BCR signatures between patients with ISR and T2DM. Further analysis of BCR profiles would enhance understanding of ISR.
format	article
author	Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong
author_facet	Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong
author_sort	Weng Ruiqiang
title	Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
title_short	Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
title_full	Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
title_fullStr	Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
title_full_unstemmed	Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
title_sort	clonal diversity of the b cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/229ca2f20cee49eba0a632a97b041eab
work_keys_str_mv	AT wengruiqiang clonaldiversityofthebcellreceptorrepertoireinpatientswithcoronaryinstentrestenosisandtype2diabetes AT liusudong clonaldiversityofthebcellreceptorrepertoireinpatientswithcoronaryinstentrestenosisandtype2diabetes AT guxiaodong clonaldiversityofthebcellreceptorrepertoireinpatientswithcoronaryinstentrestenosisandtype2diabetes AT zhongzhixiong clonaldiversityofthebcellreceptorrepertoireinpatientswithcoronaryinstentrestenosisandtype2diabetes
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Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes

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