Cooperative transition between open and closed conformations in potassium channels.

Potassium (K+) ion channels switch between open and closed conformations. The nature of this important transition was revealed by comparing the X-ray crystal structures of the MthK channel from Methanobacterium thermoautotrophicum, obtained in its open conformation, and the KcsA channel from Strepto...

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Autores principales:	Turkan Haliloglu, Nir Ben-Tal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/22a142d444704f59ab62a8d3f414b01b
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spelling	oai:doaj.org-article:22a142d444704f59ab62a8d3f414b01b2021-11-25T05:42:01ZCooperative transition between open and closed conformations in potassium channels.1553-734X1553-735810.1371/journal.pcbi.1000164https://doaj.org/article/22a142d444704f59ab62a8d3f414b01b2008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18769593/pdf/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Potassium (K+) ion channels switch between open and closed conformations. The nature of this important transition was revealed by comparing the X-ray crystal structures of the MthK channel from Methanobacterium thermoautotrophicum, obtained in its open conformation, and the KcsA channel from Streptomyces lividans, obtained in its closed conformation. We analyzed the dynamic characteristics and energetics of these homotetrameric structures in order to study the role of the intersubunit cooperativity in this transition. For this, elastic models and in silico alanine-scanning mutagenesis were used, respectively. Reassuringly, the calculations manifested motion from the open (closed) towards the closed (open) conformation. The calculations also revealed a network of dynamically and energetically coupled residues. Interestingly, the network suggests coupling between the selectivity filter and the gate, which are located at the two ends of the channel pore. Coupling between these two regions was not observed in calculations that were conducted with the monomer, which emphasizes the importance of the intersubunit interactions within the tetrameric structure for the cooperative gating behavior of the channel.Turkan HalilogluNir Ben-TalPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 4, Iss 8, p e1000164 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Turkan Haliloglu Nir Ben-Tal Cooperative transition between open and closed conformations in potassium channels.
description	Potassium (K+) ion channels switch between open and closed conformations. The nature of this important transition was revealed by comparing the X-ray crystal structures of the MthK channel from Methanobacterium thermoautotrophicum, obtained in its open conformation, and the KcsA channel from Streptomyces lividans, obtained in its closed conformation. We analyzed the dynamic characteristics and energetics of these homotetrameric structures in order to study the role of the intersubunit cooperativity in this transition. For this, elastic models and in silico alanine-scanning mutagenesis were used, respectively. Reassuringly, the calculations manifested motion from the open (closed) towards the closed (open) conformation. The calculations also revealed a network of dynamically and energetically coupled residues. Interestingly, the network suggests coupling between the selectivity filter and the gate, which are located at the two ends of the channel pore. Coupling between these two regions was not observed in calculations that were conducted with the monomer, which emphasizes the importance of the intersubunit interactions within the tetrameric structure for the cooperative gating behavior of the channel.
format	article
author	Turkan Haliloglu Nir Ben-Tal
author_facet	Turkan Haliloglu Nir Ben-Tal
author_sort	Turkan Haliloglu
title	Cooperative transition between open and closed conformations in potassium channels.
title_short	Cooperative transition between open and closed conformations in potassium channels.
title_full	Cooperative transition between open and closed conformations in potassium channels.
title_fullStr	Cooperative transition between open and closed conformations in potassium channels.
title_full_unstemmed	Cooperative transition between open and closed conformations in potassium channels.
title_sort	cooperative transition between open and closed conformations in potassium channels.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/22a142d444704f59ab62a8d3f414b01b
work_keys_str_mv	AT turkanhaliloglu cooperativetransitionbetweenopenandclosedconformationsinpotassiumchannels AT nirbental cooperativetransitionbetweenopenandclosedconformationsinpotassiumchannels
_version_	1718414505230204928

Cooperative transition between open and closed conformations in potassium channels.

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