A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum

Probiotics are clinically used for diarrhea and inflammatory bowel diseases in both humans and animals. Previous studies have shown that Clostridium tyrobutyricum (Ct) protects against intestinal dysfunction, while its regulatory function in the gut needs further investigation and the related mechan...

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Autores principales:	Zhiping Xiao, Lujie Liu, Xun Pei, Wanjing Sun, Yuyue Jin, Shang-Tian Yang, Minqi Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Clostridium tyrobutyricum1 diarrhea2 intestinal barrier function3 IL-224 Th17 cells5 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/22a575d35e2d437dbeb56c1f589ea821
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spelling	oai:doaj.org-article:22a575d35e2d437dbeb56c1f589ea8212021-12-01T20:31:42ZA Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum1664-322410.3389/fimmu.2021.758227https://doaj.org/article/22a575d35e2d437dbeb56c1f589ea8212021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.758227/fullhttps://doaj.org/toc/1664-3224Probiotics are clinically used for diarrhea and inflammatory bowel diseases in both humans and animals. Previous studies have shown that Clostridium tyrobutyricum (Ct) protects against intestinal dysfunction, while its regulatory function in the gut needs further investigation and the related mechanisms are still not fully elucidated. This study aims to further verify the protective function of Ct and reveal its underlying mechanisms in alleviating diarrhea and intestinal inflammation. Ct inhibited LPS-induced diarrhea and intestinal inflammation in the ileum. IL-22 was identified and the protective role of Ct in the ileum presented an IL-22-dependent manner according to the transcriptomic analysis and in vivo interference mice experiments. The flow cytometric analysis of immune cells in the ileum showed that Ct enhanced the proportions of Th17 cells in response to LPS. The results of in situ hybridization further verified that Ct triggered Th17 cells to produce IL-22, which combined with IL-22RA1 expressed in the epithelial cells. Moreover, Ct was unable to enhance the levels of short-chain fatty acids (SCFAs) in the ileum, suggesting that the protective role of Ct in the ileum was independent of SCFAs. This study uncovered the role of Ct in alleviating diarrhea and inflammation with the mechanism of stimulating Th17 cells in the lamina propria to produce IL-22, highlighting its potential application as a probiotic for diarrhea and inflammation in the ileum.Zhiping XiaoLujie LiuXun PeiWanjing SunYuyue JinShang-Tian YangMinqi WangFrontiers Media S.A.articleClostridium tyrobutyricum1diarrhea2intestinal barrier function3IL-224Th17 cells5Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Clostridium tyrobutyricum1 diarrhea2 intestinal barrier function3 IL-224 Th17 cells5 Immunologic diseases. Allergy RC581-607
spellingShingle	Clostridium tyrobutyricum1 diarrhea2 intestinal barrier function3 IL-224 Th17 cells5 Immunologic diseases. Allergy RC581-607 Zhiping Xiao Lujie Liu Xun Pei Wanjing Sun Yuyue Jin Shang-Tian Yang Minqi Wang A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
description	Probiotics are clinically used for diarrhea and inflammatory bowel diseases in both humans and animals. Previous studies have shown that Clostridium tyrobutyricum (Ct) protects against intestinal dysfunction, while its regulatory function in the gut needs further investigation and the related mechanisms are still not fully elucidated. This study aims to further verify the protective function of Ct and reveal its underlying mechanisms in alleviating diarrhea and intestinal inflammation. Ct inhibited LPS-induced diarrhea and intestinal inflammation in the ileum. IL-22 was identified and the protective role of Ct in the ileum presented an IL-22-dependent manner according to the transcriptomic analysis and in vivo interference mice experiments. The flow cytometric analysis of immune cells in the ileum showed that Ct enhanced the proportions of Th17 cells in response to LPS. The results of in situ hybridization further verified that Ct triggered Th17 cells to produce IL-22, which combined with IL-22RA1 expressed in the epithelial cells. Moreover, Ct was unable to enhance the levels of short-chain fatty acids (SCFAs) in the ileum, suggesting that the protective role of Ct in the ileum was independent of SCFAs. This study uncovered the role of Ct in alleviating diarrhea and inflammation with the mechanism of stimulating Th17 cells in the lamina propria to produce IL-22, highlighting its potential application as a probiotic for diarrhea and inflammation in the ileum.
format	article
author	Zhiping Xiao Lujie Liu Xun Pei Wanjing Sun Yuyue Jin Shang-Tian Yang Minqi Wang
author_facet	Zhiping Xiao Lujie Liu Xun Pei Wanjing Sun Yuyue Jin Shang-Tian Yang Minqi Wang
author_sort	Zhiping Xiao
title	A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
title_short	A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
title_full	A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
title_fullStr	A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
title_full_unstemmed	A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum
title_sort	potential probiotic for diarrhea: clostridium tyrobutyricum protects against lps-induced epithelial dysfunction via il-22 produced by th17 cells in the ileum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/22a575d35e2d437dbeb56c1f589ea821
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A Potential Probiotic for Diarrhea: Clostridium tyrobutyricum Protects Against LPS-Induced Epithelial Dysfunction via IL-22 Produced By Th17 Cells in the Ileum

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