Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.

Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigi...

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Autores principales:	Chee-Hoo Yip, Sobina Mahalingam, Kiew-Lian Wan, Sheila Nathan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/22a6340d97874687b2da2ce495e7cd91
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spelling	oai:doaj.org-article:22a6340d97874687b2da2ce495e7cd912021-12-02T20:10:11ZProdigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.1932-620310.1371/journal.pone.0253445https://doaj.org/article/22a6340d97874687b2da2ce495e7cd912021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253445https://doaj.org/toc/1932-6203Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.Chee-Hoo YipSobina MahalingamKiew-Lian WanSheila NathanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253445 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chee-Hoo Yip Sobina Mahalingam Kiew-Lian Wan Sheila Nathan Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
description	Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens, particularly in microbial competition through its antimicrobial activity, towards the growth and secreted virulence factors of four clinical pathogenic bacteria (methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa) as well as Staphylococcus aureus and Escherichia coli. Prodigiosin was first extracted from S. marcescens and its purity confirmed by absorption spectrum, high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The extracted prodigiosin was antagonistic towards all the tested bacteria. A disc-diffusion assay showed that prodigiosin is more selective towards Gram-positive bacteria and inhibited the growth of MRSA, S. aureus and E. faecalis and Gram-negative E. coli. A minimum inhibitory concentration of 10 μg/μL of prodigiosin was required to inhibit the growth of S. aureus, E. coli and E. faecalis whereas > 10 μg/μL was required to inhibit MRSA growth. We further assessed the effect of prodigiosin towards bacterial virulence factors such as haemolysin and production of protease as well as on biofilm formation. Prodigiosin did not inhibit haemolysis activity of clinically associated bacteria but was able to reduce protease activity for MRSA, E. coli and E. faecalis as well as decrease E. faecalis, Salmonella Typhimurium and E. coli biofilm formation. Results of this study show that in addition to its role in inhibiting bacterial growth, prodigiosin also inhibits the bacterial virulence factor protease production and biofilm formation, two strategies employed by bacteria in response to microbial competition. As clinical pathogens were more resistant to prodigiosin, we propose that prodigiosin is physiologically important for S. marcescens to compete against other bacteria in its natural soil and surface water environments.
format	article
author	Chee-Hoo Yip Sobina Mahalingam Kiew-Lian Wan Sheila Nathan
author_facet	Chee-Hoo Yip Sobina Mahalingam Kiew-Lian Wan Sheila Nathan
author_sort	Chee-Hoo Yip
title	Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
title_short	Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
title_full	Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
title_fullStr	Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
title_full_unstemmed	Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
title_sort	prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/22a6340d97874687b2da2ce495e7cd91
work_keys_str_mv	AT cheehooyip prodigiosininhibitsbacterialgrowthandvirulencefactorsasapotentialphysiologicalresponsetointerspeciescompetition AT sobinamahalingam prodigiosininhibitsbacterialgrowthandvirulencefactorsasapotentialphysiologicalresponsetointerspeciescompetition AT kiewlianwan prodigiosininhibitsbacterialgrowthandvirulencefactorsasapotentialphysiologicalresponsetointerspeciescompetition AT sheilanathan prodigiosininhibitsbacterialgrowthandvirulencefactorsasapotentialphysiologicalresponsetointerspeciescompetition
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Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition.

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