DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant

Abstract Several agonists to CD40 have shown to induce acquired immune responses. Here, we developed and evaluated the rolling circle amplification (RCA) products that are based on anti-CD40 DNA aptamers as a novel vaccine adjuvant. First, we developed DNA aptamers with specific binding affinity to...

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Autores principales:	Adil S. Al-Ogaili, Rohana Liyanage, Jack O. Lay, Tieshan Jiang, Christine N. Vuong, Shilpi Agrawal, Thallapuranam Krishnaswamy Suresh Kumar, Luc R. Berghman, Billy M. Hargis, Young Min Kwon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/22abb468b59f435eb360b09b8463f8c6
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spelling	oai:doaj.org-article:22abb468b59f435eb360b09b8463f8c62021-12-02T13:58:15ZDNA aptamer-based rolling circle amplification product as a novel immunological adjuvant10.1038/s41598-020-79420-w2045-2322https://doaj.org/article/22abb468b59f435eb360b09b8463f8c62020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79420-whttps://doaj.org/toc/2045-2322Abstract Several agonists to CD40 have shown to induce acquired immune responses. Here, we developed and evaluated the rolling circle amplification (RCA) products that are based on anti-CD40 DNA aptamers as a novel vaccine adjuvant. First, we developed DNA aptamers with specific binding affinity to chicken CD40 extra domain (chCD40ED). Next, we prepared the RCA products that consist of these aptamers to increase the spanning space and overall binding affinity to chCD40ED. Using 8 DNA aptamer candidates, 4 aptamer-based RCA products (aptamer RCAs) were generated, each consisting of two distinct aptamers. We demonstrated that all 4 aptamer RCAs significantly induced the signal transduction in chicken HD11 macrophage cell line (p < 0.05). Finally, we conjugated one of the aptamer RCAs (Aptamer RCA II) to M2e epitope peptide of influenza virus as a model hapten, and the immune complex was injected to chickens. Aptamer RCA II stimulated anti-M2e IgG antibody production to the level significantly higher as compared to the control (M2e epitope alone; p < 0.05). The results of our work suggest that aptamer RCA is a novel platform to boost the efficacy of vaccines, which might find broad applications to other antigens beyond M2e epitope evaluated in this study using chicken infection model.Adil S. Al-OgailiRohana LiyanageJack O. LayTieshan JiangChristine N. VuongShilpi AgrawalThallapuranam Krishnaswamy Suresh KumarLuc R. BerghmanBilly M. HargisYoung Min KwonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adil S. Al-Ogaili Rohana Liyanage Jack O. Lay Tieshan Jiang Christine N. Vuong Shilpi Agrawal Thallapuranam Krishnaswamy Suresh Kumar Luc R. Berghman Billy M. Hargis Young Min Kwon DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
description	Abstract Several agonists to CD40 have shown to induce acquired immune responses. Here, we developed and evaluated the rolling circle amplification (RCA) products that are based on anti-CD40 DNA aptamers as a novel vaccine adjuvant. First, we developed DNA aptamers with specific binding affinity to chicken CD40 extra domain (chCD40ED). Next, we prepared the RCA products that consist of these aptamers to increase the spanning space and overall binding affinity to chCD40ED. Using 8 DNA aptamer candidates, 4 aptamer-based RCA products (aptamer RCAs) were generated, each consisting of two distinct aptamers. We demonstrated that all 4 aptamer RCAs significantly induced the signal transduction in chicken HD11 macrophage cell line (p < 0.05). Finally, we conjugated one of the aptamer RCAs (Aptamer RCA II) to M2e epitope peptide of influenza virus as a model hapten, and the immune complex was injected to chickens. Aptamer RCA II stimulated anti-M2e IgG antibody production to the level significantly higher as compared to the control (M2e epitope alone; p < 0.05). The results of our work suggest that aptamer RCA is a novel platform to boost the efficacy of vaccines, which might find broad applications to other antigens beyond M2e epitope evaluated in this study using chicken infection model.
format	article
author	Adil S. Al-Ogaili Rohana Liyanage Jack O. Lay Tieshan Jiang Christine N. Vuong Shilpi Agrawal Thallapuranam Krishnaswamy Suresh Kumar Luc R. Berghman Billy M. Hargis Young Min Kwon
author_facet	Adil S. Al-Ogaili Rohana Liyanage Jack O. Lay Tieshan Jiang Christine N. Vuong Shilpi Agrawal Thallapuranam Krishnaswamy Suresh Kumar Luc R. Berghman Billy M. Hargis Young Min Kwon
author_sort	Adil S. Al-Ogaili
title	DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
title_short	DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
title_full	DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
title_fullStr	DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
title_full_unstemmed	DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant
title_sort	dna aptamer-based rolling circle amplification product as a novel immunological adjuvant
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/22abb468b59f435eb360b09b8463f8c6
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DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant

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