Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery

Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery

Shiao-Wen Tsai,1 Ding-Syuan Yu,2 Shu-Wei Tsao,1 Fu-Yin Hsu2,3 1Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan; 2Department of Life Science, 3Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan Abstract: Hy...

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Autores principales: Tsai SW, Yu DS, Tsao SW, Hsu FY
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2013
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/22b14d7d38a94263b29aba39a4f0a9d0
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spelling oai:doaj.org-article:22b14d7d38a94263b29aba39a4f0a9d02021-12-02T02:49:27ZHyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery1176-91141178-2013https://doaj.org/article/22b14d7d38a94263b29aba39a4f0a9d02013-07-01T00:00:00Zhttp://www.dovepress.com/hyaluronanndashcisplatin-conjugate-nanoparticles-embedded-in-eudragit--a13540https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Shiao-Wen Tsai,1 Ding-Syuan Yu,2 Shu-Wei Tsao,1 Fu-Yin Hsu2,3 1Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan; 2Department of Life Science, 3Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan Abstract: Hyaluronan–cisplatin conjugate nanoparticles (HCNPs) were chosen as colon-targeting drug-delivery carriers due to the observation that a variety of malignant tumors overexpress hyaluronan receptors. HCNPs were prepared by mixing cisplatin with a hyaluronan solution, followed by dialysis to remove trace elements. The cells treated with HCNPs showed significantly lower viability than those treated with cisplatin alone. HCNPs were entrapped in Eudragit S100-coated pectinate/alginate microbeads (PAMs) by using an electrospray method and a polyelectrolyte multilayer-coating technique in aqueous solution. The release profile of HCNPs from Eudragit S100-coated HCNP-PAMs was pH-dependent. The percentage of 24-hour drug release was approximately 25.1% and 39.7% in pH 1.2 and pH 4.5 media, respectively. However, the percentage of drug released quickly rose to 75.6% at pH 7.4. Moreover, the result of an in vivo nephrotoxicity study demonstrated that Eudragit S100-coated HCNP-PAMs treatment could mitigate the nephrotoxicity that resulted from cisplatin. From these results, it can be concluded that Eudragit S100-coated HCNP-PAMs are promising carriers for colon-specific drug delivery. Keywords: hyaluronan, cisplatin, pectin, alginate, pH-dependent, drug deliveryTsai SWYu DSTsao SWHsu FYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2399-2407 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Tsai SW
Yu DS
Tsao SW
Hsu FY
Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
description Shiao-Wen Tsai,1 Ding-Syuan Yu,2 Shu-Wei Tsao,1 Fu-Yin Hsu2,3 1Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan; 2Department of Life Science, 3Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan Abstract: Hyaluronan–cisplatin conjugate nanoparticles (HCNPs) were chosen as colon-targeting drug-delivery carriers due to the observation that a variety of malignant tumors overexpress hyaluronan receptors. HCNPs were prepared by mixing cisplatin with a hyaluronan solution, followed by dialysis to remove trace elements. The cells treated with HCNPs showed significantly lower viability than those treated with cisplatin alone. HCNPs were entrapped in Eudragit S100-coated pectinate/alginate microbeads (PAMs) by using an electrospray method and a polyelectrolyte multilayer-coating technique in aqueous solution. The release profile of HCNPs from Eudragit S100-coated HCNP-PAMs was pH-dependent. The percentage of 24-hour drug release was approximately 25.1% and 39.7% in pH 1.2 and pH 4.5 media, respectively. However, the percentage of drug released quickly rose to 75.6% at pH 7.4. Moreover, the result of an in vivo nephrotoxicity study demonstrated that Eudragit S100-coated HCNP-PAMs treatment could mitigate the nephrotoxicity that resulted from cisplatin. From these results, it can be concluded that Eudragit S100-coated HCNP-PAMs are promising carriers for colon-specific drug delivery. Keywords: hyaluronan, cisplatin, pectin, alginate, pH-dependent, drug delivery
format article
author Tsai SW
Yu DS
Tsao SW
Hsu FY
author_facet Tsai SW
Yu DS
Tsao SW
Hsu FY
author_sort Tsai SW
title Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
title_short Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
title_full Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
title_fullStr Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
title_full_unstemmed Hyaluronan–cisplatin conjugate nanoparticles embedded in Eudragit S100-coated pectin/alginate microbeads for colon drug delivery
title_sort hyaluronan–cisplatin conjugate nanoparticles embedded in eudragit s100-coated pectin/alginate microbeads for colon drug delivery
publisher Dove Medical Press
publishDate 2013
url https://doaj.org/article/22b14d7d38a94263b29aba39a4f0a9d0
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AT yuds hyaluronanndashcisplatinconjugatenanoparticlesembeddedineudragits100coatedpectinalginatemicrobeadsforcolondrugdelivery
AT tsaosw hyaluronanndashcisplatinconjugatenanoparticlesembeddedineudragits100coatedpectinalginatemicrobeadsforcolondrugdelivery
AT hsufy hyaluronanndashcisplatinconjugatenanoparticlesembeddedineudragits100coatedpectinalginatemicrobeadsforcolondrugdelivery
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