chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A

Granulosa cell (GC) proliferation provides essential conditions for ovulation in animals. A previous study showed that DENND1A plays a significant role in polycystic ovary syndrome. However, the modulation of DENND1A in GCs remains unclear. Our previous integrated analysis of miRNA–mRNA revealed tha...

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Autores principales:	Yufang Liu, Yulin Chen, Zuyang Zhou, Xiaoyun He, Lin Tao, Yanting Jiang, Rong Lan, Qionghua Hong, Mingxing Chu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chi-miR-324-3p DENND1A granulosa cells proliferation goat Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/22b39ec0597a4b8c952e1b00d1cd769e
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spelling	oai:doaj.org-article:22b39ec0597a4b8c952e1b00d1cd769e2021-11-18T05:35:06Zchi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A2297-176910.3389/fvets.2021.732440https://doaj.org/article/22b39ec0597a4b8c952e1b00d1cd769e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.732440/fullhttps://doaj.org/toc/2297-1769Granulosa cell (GC) proliferation provides essential conditions for ovulation in animals. A previous study showed that DENND1A plays a significant role in polycystic ovary syndrome. However, the modulation of DENND1A in GCs remains unclear. Our previous integrated analysis of miRNA–mRNA revealed that the 3'-untranslated region of DENND1A could be a target of chi-miR-324-3p. In this study, we used quantitative reverse transcription polymerase chain reaction (RT-qPCR) to investigate DENND1A expression in ovarian tissues of high- and low-yielding goats. Furthermore, dual-fluorescent reporter vector experiments, Cell Counting Kit-8 (CCK-8) assay, and RT-qPCR were used to elucidate the regulatory pathway of chi-miR-324-3p-DENND1A in GCs. The results revealed an opposite tendency between the expressions of chi-miR-324-3p and DENND1A in the ovaries of high- and low-yielding goats. The CCK-8 assay indicated that chi-miR-324-3p overexpression significantly suppressed GC proliferation, whereas chi-miR-324-3p inhibition promoted GC proliferation. In addition, the expressions of GC proliferation markers LHR, Cylin D2, and CDK4 showed the same tendency. The dual-fluorescent reporter assay revealed that chi-miR-324-3p directly targeted DENND1A, and the RT-qPCR results revealed that DENND1A expression was inhibited by chi-miR-324-3p. In summary, chi-miR-324-3p inhibited the proliferation of GCs by targeting DENND1A.Yufang LiuYufang LiuYulin ChenYulin ChenZuyang ZhouZuyang ZhouXiaoyun HeLin TaoYanting JiangRong LanQionghua HongMingxing ChuFrontiers Media S.A.articlechi-miR-324-3pDENND1Agranulosa cellsproliferationgoatVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chi-miR-324-3p DENND1A granulosa cells proliferation goat Veterinary medicine SF600-1100
spellingShingle	chi-miR-324-3p DENND1A granulosa cells proliferation goat Veterinary medicine SF600-1100 Yufang Liu Yufang Liu Yulin Chen Yulin Chen Zuyang Zhou Zuyang Zhou Xiaoyun He Lin Tao Yanting Jiang Rong Lan Qionghua Hong Mingxing Chu chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
description	Granulosa cell (GC) proliferation provides essential conditions for ovulation in animals. A previous study showed that DENND1A plays a significant role in polycystic ovary syndrome. However, the modulation of DENND1A in GCs remains unclear. Our previous integrated analysis of miRNA–mRNA revealed that the 3'-untranslated region of DENND1A could be a target of chi-miR-324-3p. In this study, we used quantitative reverse transcription polymerase chain reaction (RT-qPCR) to investigate DENND1A expression in ovarian tissues of high- and low-yielding goats. Furthermore, dual-fluorescent reporter vector experiments, Cell Counting Kit-8 (CCK-8) assay, and RT-qPCR were used to elucidate the regulatory pathway of chi-miR-324-3p-DENND1A in GCs. The results revealed an opposite tendency between the expressions of chi-miR-324-3p and DENND1A in the ovaries of high- and low-yielding goats. The CCK-8 assay indicated that chi-miR-324-3p overexpression significantly suppressed GC proliferation, whereas chi-miR-324-3p inhibition promoted GC proliferation. In addition, the expressions of GC proliferation markers LHR, Cylin D2, and CDK4 showed the same tendency. The dual-fluorescent reporter assay revealed that chi-miR-324-3p directly targeted DENND1A, and the RT-qPCR results revealed that DENND1A expression was inhibited by chi-miR-324-3p. In summary, chi-miR-324-3p inhibited the proliferation of GCs by targeting DENND1A.
format	article
author	Yufang Liu Yufang Liu Yulin Chen Yulin Chen Zuyang Zhou Zuyang Zhou Xiaoyun He Lin Tao Yanting Jiang Rong Lan Qionghua Hong Mingxing Chu
author_facet	Yufang Liu Yufang Liu Yulin Chen Yulin Chen Zuyang Zhou Zuyang Zhou Xiaoyun He Lin Tao Yanting Jiang Rong Lan Qionghua Hong Mingxing Chu
author_sort	Yufang Liu
title	chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
title_short	chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
title_full	chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
title_fullStr	chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
title_full_unstemmed	chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A
title_sort	chi-mir-324-3p regulates goat granulosa cell proliferation by targeting dennd1a
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/22b39ec0597a4b8c952e1b00d1cd769e
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chi-miR-324-3p Regulates Goat Granulosa Cell Proliferation by Targeting DENND1A

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