Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.

The present study was conducted to investigate the effects of maternal zearalenone (ZEN) exposure on the intestine of pregnant Sprague-Dawley (SD) rats and its offspring. Ninety-six pregnant SD rats were randomly divided into four groups and were fed with diets containing ZEN at concentrations of 0....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Min Liu, Rui Gao, Qingwei Meng, Yuanyuan Zhang, Chongpeng Bi, Anshan Shan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/22bc2e86aba24979a9c956af3ed5b480
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:22bc2e86aba24979a9c956af3ed5b480
record_format	dspace
spelling	oai:doaj.org-article:22bc2e86aba24979a9c956af3ed5b4802021-11-25T06:02:25ZToxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.1932-620310.1371/journal.pone.0106412https://doaj.org/article/22bc2e86aba24979a9c956af3ed5b4802014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25180673/?tool=EBIhttps://doaj.org/toc/1932-6203The present study was conducted to investigate the effects of maternal zearalenone (ZEN) exposure on the intestine of pregnant Sprague-Dawley (SD) rats and its offspring. Ninety-six pregnant SD rats were randomly divided into four groups and were fed with diets containing ZEN at concentrations of 0.3 mg/kg, 48.5 mg/kg, 97.6 mg/kg or 146.0 mg/kg from gestation days (GD) 1 to 7. All rats were fed with mycotoxin-free diet until their offspring were weaned at three weeks of age. The small intestinal fragments from pregnant rats at GD8, weaned dams and pups were collected and studied for toxic effects of ZEN on antioxidant status, immune response, expression of junction proteins, and morphology. The results showed that ZEN induced oxidative stress, affected the villous structure and reduced the expression of junction proteins claudin-4, occludin and connexin43 (Cx43) in a dose-dependent manner in pregnant rats. Different effects on the expression of cytokines were also observed both in mRNA and protein levels in these pregnant groups. Ingestion of high levels of ZEN caused irreversible damage in weaned dams, such as oxidative stress, decreased villi hight and low expression of junction proteins and cytokines. Decreased expression of jejunal interleukin-8 (IL-8) and increased expression of gastrointestinal glutathione peroxidase (GPx2) mRNA were detected in weaned offspring, indicating long-term damage caused by maternal ZEN. We also found that the Nrf2 expression both in mRNA and protein levels were up-regulated in the ZEN-treated groups of pregnant dams and the high-dose of ZEN group of weaned dams. The data indicate that modulation of Nrf2-mediated pathway is one of mechanism via which ZEN affects gut wall antioxidant and inflammatory responses.Min LiuRui GaoQingwei MengYuanyuan ZhangChongpeng BiAnshan ShanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106412 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Liu Rui Gao Qingwei Meng Yuanyuan Zhang Chongpeng Bi Anshan Shan Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
description	The present study was conducted to investigate the effects of maternal zearalenone (ZEN) exposure on the intestine of pregnant Sprague-Dawley (SD) rats and its offspring. Ninety-six pregnant SD rats were randomly divided into four groups and were fed with diets containing ZEN at concentrations of 0.3 mg/kg, 48.5 mg/kg, 97.6 mg/kg or 146.0 mg/kg from gestation days (GD) 1 to 7. All rats were fed with mycotoxin-free diet until their offspring were weaned at three weeks of age. The small intestinal fragments from pregnant rats at GD8, weaned dams and pups were collected and studied for toxic effects of ZEN on antioxidant status, immune response, expression of junction proteins, and morphology. The results showed that ZEN induced oxidative stress, affected the villous structure and reduced the expression of junction proteins claudin-4, occludin and connexin43 (Cx43) in a dose-dependent manner in pregnant rats. Different effects on the expression of cytokines were also observed both in mRNA and protein levels in these pregnant groups. Ingestion of high levels of ZEN caused irreversible damage in weaned dams, such as oxidative stress, decreased villi hight and low expression of junction proteins and cytokines. Decreased expression of jejunal interleukin-8 (IL-8) and increased expression of gastrointestinal glutathione peroxidase (GPx2) mRNA were detected in weaned offspring, indicating long-term damage caused by maternal ZEN. We also found that the Nrf2 expression both in mRNA and protein levels were up-regulated in the ZEN-treated groups of pregnant dams and the high-dose of ZEN group of weaned dams. The data indicate that modulation of Nrf2-mediated pathway is one of mechanism via which ZEN affects gut wall antioxidant and inflammatory responses.
format	article
author	Min Liu Rui Gao Qingwei Meng Yuanyuan Zhang Chongpeng Bi Anshan Shan
author_facet	Min Liu Rui Gao Qingwei Meng Yuanyuan Zhang Chongpeng Bi Anshan Shan
author_sort	Min Liu
title	Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
title_short	Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
title_full	Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
title_fullStr	Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
title_full_unstemmed	Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
title_sort	toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/22bc2e86aba24979a9c956af3ed5b480
work_keys_str_mv	AT minliu toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats AT ruigao toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats AT qingweimeng toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats AT yuanyuanzhang toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats AT chongpengbi toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats AT anshanshan toxiceffectsofmaternalzearalenoneexposureonintestinaloxidativestressbarrierfunctionimmunologicalandmorphologicalchangesinrats
_version_	1718414268394635264

Toxic effects of maternal zearalenone exposure on intestinal oxidative stress, barrier function, immunological and morphological changes in rats.

Ejemplares similares