Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.

<h4>Background</h4>Male-factor infertility is a common condition, and etiology is unknown for a high proportion of cases. Abnormal epigenetic programming of the germline is proposed as a possible mechanism compromising spermatogenesis of some men currently diagnosed with idiopathic infer...

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Autores principales:	Sahar Houshdaran, Victoria K Cortessis, Kimberly Siegmund, Allen Yang, Peter W Laird, Rebecca Z Sokol
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/22c4699e7dba4875bc2049af3e3c75a2
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spelling	oai:doaj.org-article:22c4699e7dba4875bc2049af3e3c75a22021-11-25T06:13:42ZWidespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.1932-620310.1371/journal.pone.0001289https://doaj.org/article/22c4699e7dba4875bc2049af3e3c75a22007-12-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001289https://doaj.org/toc/1932-6203<h4>Background</h4>Male-factor infertility is a common condition, and etiology is unknown for a high proportion of cases. Abnormal epigenetic programming of the germline is proposed as a possible mechanism compromising spermatogenesis of some men currently diagnosed with idiopathic infertility. During germ cell maturation and gametogenesis, cells of the germ line undergo extensive epigenetic reprogramming. This process involves widespread erasure of somatic-like patterns of DNA methylation followed by establishment of sex-specific patterns by de novo DNA methylation. Incomplete reprogramming of the male germ line could, in theory, result in both altered sperm DNA methylation and compromised spermatogenesis.<h4>Methodology/principal finding</h4>We determined concentration, motility and morphology of sperm in semen samples collected by male members of couples attending an infertility clinic. Using MethyLight and Illumina assays we measured methylation of DNA isolated from purified sperm from the same samples. Methylation at numerous sequences was elevated in DNA from poor quality sperm.<h4>Conclusions</h4>This is the first report of a broad epigenetic defect associated with abnormal semen parameters. Our results suggest that the underlying mechanism for these epigenetic changes may be improper erasure of DNA methylation during epigenetic reprogramming of the male germ line.Sahar HoushdaranVictoria K CortessisKimberly SiegmundAllen YangPeter W LairdRebecca Z SokolPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 12, p e1289 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sahar Houshdaran Victoria K Cortessis Kimberly Siegmund Allen Yang Peter W Laird Rebecca Z Sokol Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
description	<h4>Background</h4>Male-factor infertility is a common condition, and etiology is unknown for a high proportion of cases. Abnormal epigenetic programming of the germline is proposed as a possible mechanism compromising spermatogenesis of some men currently diagnosed with idiopathic infertility. During germ cell maturation and gametogenesis, cells of the germ line undergo extensive epigenetic reprogramming. This process involves widespread erasure of somatic-like patterns of DNA methylation followed by establishment of sex-specific patterns by de novo DNA methylation. Incomplete reprogramming of the male germ line could, in theory, result in both altered sperm DNA methylation and compromised spermatogenesis.<h4>Methodology/principal finding</h4>We determined concentration, motility and morphology of sperm in semen samples collected by male members of couples attending an infertility clinic. Using MethyLight and Illumina assays we measured methylation of DNA isolated from purified sperm from the same samples. Methylation at numerous sequences was elevated in DNA from poor quality sperm.<h4>Conclusions</h4>This is the first report of a broad epigenetic defect associated with abnormal semen parameters. Our results suggest that the underlying mechanism for these epigenetic changes may be improper erasure of DNA methylation during epigenetic reprogramming of the male germ line.
format	article
author	Sahar Houshdaran Victoria K Cortessis Kimberly Siegmund Allen Yang Peter W Laird Rebecca Z Sokol
author_facet	Sahar Houshdaran Victoria K Cortessis Kimberly Siegmund Allen Yang Peter W Laird Rebecca Z Sokol
author_sort	Sahar Houshdaran
title	Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
title_short	Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
title_full	Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
title_fullStr	Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
title_full_unstemmed	Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.
title_sort	widespread epigenetic abnormalities suggest a broad dna methylation erasure defect in abnormal human sperm.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/22c4699e7dba4875bc2049af3e3c75a2
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Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm.

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