L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis

High sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the pres...

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Autores principales:	Shuming Mo, Jinhui Li, Bin Li, Muhammad Kashif, Shiqing Nie, Jianping Liao, Guijiao Su, Qiong Jiang, Bing Yan, Chengjian Jiang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sulfate-reduction gene families subtropical mangrove sediment sulfide metagenomics L-cysteine synthase Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/22c58d4cd50c4caca895aa2269201b1d
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spelling	oai:doaj.org-article:22c58d4cd50c4caca895aa2269201b1d2021-11-11T19:55:51ZL-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis10.3390/w132130532073-4441https://doaj.org/article/22c58d4cd50c4caca895aa2269201b1d2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/21/3053https://doaj.org/toc/2073-4441High sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the present study, the sulfide biotransformation in subtropical mangroves ecosystem was accurately evaluated using metagenomic sequencing and quantitative polymerase chain reaction analysis. Most abundant genes were related to the organic sulfur transformation. Furthermore, an ecological model of sulfide conversion was constructed. Total phosphorus was the dominant environmental factor that drove the sulfur cycle and microbial communities. We compared mangrove and non-mangrove soils and found that the former enhanced metabolism that was related to sulfate reduction when compared to the latter. Total organic carbon, total organic nitrogen, iron, and available sulfur were the key environmental factors that effectively influenced the dissimilatory sulfate reduction. The taxonomic assignment of dissimilatory sulfate-reducing genes revealed that <i>Desulfobacterales</i> and <i>Chromatiales</i> were mainly responsible for sulfate reduction. <i>Chromatiales</i> were most sensitive to environmental factors. The high abundance of <i>cysE</i> and <i>cysK</i> could contribute to the coping of the microbial community with the toxic sulfide produced by <i>Desulfobacterales</i>. Collectively, these findings provided a theoretical basis for the mechanism of the sulfur cycle in subtropical mangrove ecosystems.Shuming MoJinhui LiBin LiMuhammad KashifShiqing NieJianping LiaoGuijiao SuQiong JiangBing YanChengjian JiangMDPI AGarticlesulfate-reduction gene familiessubtropical mangrove sedimentsulfidemetagenomicsL-cysteine synthaseHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3053, p 3053 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sulfate-reduction gene families subtropical mangrove sediment sulfide metagenomics L-cysteine synthase Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	sulfate-reduction gene families subtropical mangrove sediment sulfide metagenomics L-cysteine synthase Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
description	High sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the present study, the sulfide biotransformation in subtropical mangroves ecosystem was accurately evaluated using metagenomic sequencing and quantitative polymerase chain reaction analysis. Most abundant genes were related to the organic sulfur transformation. Furthermore, an ecological model of sulfide conversion was constructed. Total phosphorus was the dominant environmental factor that drove the sulfur cycle and microbial communities. We compared mangrove and non-mangrove soils and found that the former enhanced metabolism that was related to sulfate reduction when compared to the latter. Total organic carbon, total organic nitrogen, iron, and available sulfur were the key environmental factors that effectively influenced the dissimilatory sulfate reduction. The taxonomic assignment of dissimilatory sulfate-reducing genes revealed that <i>Desulfobacterales</i> and <i>Chromatiales</i> were mainly responsible for sulfate reduction. <i>Chromatiales</i> were most sensitive to environmental factors. The high abundance of <i>cysE</i> and <i>cysK</i> could contribute to the coping of the microbial community with the toxic sulfide produced by <i>Desulfobacterales</i>. Collectively, these findings provided a theoretical basis for the mechanism of the sulfur cycle in subtropical mangrove ecosystems.
format	article
author	Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang
author_facet	Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang
author_sort	Shuming Mo
title	L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
title_short	L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
title_full	L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
title_fullStr	L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
title_full_unstemmed	L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
title_sort	l-cysteine synthase enhanced sulfide biotransformation in subtropical marine mangrove sediments as revealed by metagenomics analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/22c58d4cd50c4caca895aa2269201b1d
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L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis

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