Extracellular Vesicles from Fusarium graminearum Contain Protein Effectors Expressed during Infection of Corn

Fusarium graminearum (Fgr) is a devastating filamentous fungal pathogen that causes diseases in cereals, while producing mycotoxins that are toxic for humans and animals, and render grains unusable. Low efficiency in managing Fgr</...

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Autores principales:	Donovan Garcia-Ceron, Rohan G. T. Lowe, James A. McKenna, Linda M. Brain, Charlotte S. Dawson, Bethany Clark, Oliver Berkowitz, Pierre Faou, James Whelan, Mark R. Bleackley, Marilyn A. Anderson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	EVs fungal extracellular vesicles fungi <i>Fusarium</i> <i>graminearum</i> protein effectors unconventional secretion Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/22c965fb42614a228da46731251d95dc
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spelling	oai:doaj.org-article:22c965fb42614a228da46731251d95dc2021-11-25T18:06:22ZExtracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn10.3390/jof71109772309-608Xhttps://doaj.org/article/22c965fb42614a228da46731251d95dc2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/977https://doaj.org/toc/2309-608X<i>Fusarium</i> <i>graminearum</i> (<i>Fgr</i>) is a devastating filamentous fungal pathogen that causes diseases in cereals, while producing mycotoxins that are toxic for humans and animals, and render grains unusable. Low efficiency in managing <i>Fgr</i> poses a constant need for identifying novel control mechanisms. Evidence that fungal extracellular vesicles (EVs) from pathogenic yeast have a role in human disease led us to question whether this is also true for fungal plant pathogens. We separated EVs from <i>Fgr</i> and performed a proteomic analysis to determine if EVs carry proteins with potential roles in pathogenesis. We revealed that protein effectors, which are crucial for fungal virulence, were detected in EV preparations and some of them did not contain predicted secretion signals. Furthermore, a transcriptomic analysis of corn (<i>Zea</i> <i>mays</i>) plants infected by <i>Fgr</i> revealed that the genes of some of the effectors were highly expressed in vivo, suggesting that the <i>Fgr</i> EVs are a mechanism for the unconventional secretion of effectors and virulence factors. Our results expand the knowledge on fungal EVs in plant pathogenesis and cross-kingdom communication, and may contribute to the discovery of new antifungals.Donovan Garcia-CeronRohan G. T. LoweJames A. McKennaLinda M. BrainCharlotte S. DawsonBethany ClarkOliver BerkowitzPierre FaouJames WhelanMark R. BleackleyMarilyn A. AndersonMDPI AGarticleEVsfungal extracellular vesiclesfungi<i>Fusarium</i> <i>graminearum</i>protein effectorsunconventional secretionBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 977, p 977 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	EVs fungal extracellular vesicles fungi <i>Fusarium</i> <i>graminearum</i> protein effectors unconventional secretion Biology (General) QH301-705.5
spellingShingle	EVs fungal extracellular vesicles fungi <i>Fusarium</i> <i>graminearum</i> protein effectors unconventional secretion Biology (General) QH301-705.5 Donovan Garcia-Ceron Rohan G. T. Lowe James A. McKenna Linda M. Brain Charlotte S. Dawson Bethany Clark Oliver Berkowitz Pierre Faou James Whelan Mark R. Bleackley Marilyn A. Anderson Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
description	<i>Fusarium</i> <i>graminearum</i> (<i>Fgr</i>) is a devastating filamentous fungal pathogen that causes diseases in cereals, while producing mycotoxins that are toxic for humans and animals, and render grains unusable. Low efficiency in managing <i>Fgr</i> poses a constant need for identifying novel control mechanisms. Evidence that fungal extracellular vesicles (EVs) from pathogenic yeast have a role in human disease led us to question whether this is also true for fungal plant pathogens. We separated EVs from <i>Fgr</i> and performed a proteomic analysis to determine if EVs carry proteins with potential roles in pathogenesis. We revealed that protein effectors, which are crucial for fungal virulence, were detected in EV preparations and some of them did not contain predicted secretion signals. Furthermore, a transcriptomic analysis of corn (<i>Zea</i> <i>mays</i>) plants infected by <i>Fgr</i> revealed that the genes of some of the effectors were highly expressed in vivo, suggesting that the <i>Fgr</i> EVs are a mechanism for the unconventional secretion of effectors and virulence factors. Our results expand the knowledge on fungal EVs in plant pathogenesis and cross-kingdom communication, and may contribute to the discovery of new antifungals.
format	article
author	Donovan Garcia-Ceron Rohan G. T. Lowe James A. McKenna Linda M. Brain Charlotte S. Dawson Bethany Clark Oliver Berkowitz Pierre Faou James Whelan Mark R. Bleackley Marilyn A. Anderson
author_facet	Donovan Garcia-Ceron Rohan G. T. Lowe James A. McKenna Linda M. Brain Charlotte S. Dawson Bethany Clark Oliver Berkowitz Pierre Faou James Whelan Mark R. Bleackley Marilyn A. Anderson
author_sort	Donovan Garcia-Ceron
title	Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
title_short	Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
title_full	Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
title_fullStr	Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
title_full_unstemmed	Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn
title_sort	extracellular vesicles from <i>fusarium</i> <i>graminearum</i> contain protein effectors expressed during infection of corn
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/22c965fb42614a228da46731251d95dc
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Extracellular Vesicles from <i>Fusarium</i> <i>graminearum</i> Contain Protein Effectors Expressed during Infection of Corn

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