Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats

Monireh Azizi,1 Naser Abbasi,2,3 Mojtaba Mohamadpour,4 Salar Bakhtiyari,5 Sirous Asadi,4 Ehsan Shirzadpour,5 Ali Aidy,2 Mahmoud Mohamadpour,5 Mansour Amraei2,6 1Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 2Biotechnology and Medicinal Plants Research C...

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Autores principales:	Azizi M, Abbasi N, Mohamadpour M, Bakhtiyari S, Asadi S, Shirzadpour E, Aidy A, Amraei M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Crocus Sativus L. Alcohol Kidney Liver Rat Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/22cc5d7526e242029802695959bd536a
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spelling	oai:doaj.org-article:22cc5d7526e242029802695959bd536a2021-12-02T09:10:45ZInvestigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats1178-7031https://doaj.org/article/22cc5d7526e242029802695959bd536a2019-10-01T00:00:00Zhttps://www.dovepress.com/investigating-the-effect-of-crocus-sativus-l-petal-hydroalcoholic-extr-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Monireh Azizi,1 Naser Abbasi,2,3 Mojtaba Mohamadpour,4 Salar Bakhtiyari,5 Sirous Asadi,4 Ehsan Shirzadpour,5 Ali Aidy,2 Mahmoud Mohamadpour,5 Mansour Amraei2,6 1Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 2Biotechnology and Medicinal Plants Research Center, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 3Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 4Student Research Committee, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 5Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 6Department of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IranCorrespondence: Mansour AmraeiDepartment of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Banganjab Street, PO Box 6939177143, Ilam, IranTel +98 843 223 5724Fax +98 843 222 7136Email amraei.mansour@yahoo.comBackground: Studies have shown that consumption of high levels of alcohol causes many negative effects on the liver and kidneys where antioxidant ingredients can be a proper solution to reducing the resulting damages. So, the present study investigated the effect of hydroalcoholic extract of Crocus sativus L. (saffron) petal with antioxidant properties on the changes in inflammatory and enzymatic indices resulting from alcohol use in the male rats’ kidney and liver.Materials and methods: After preparing the extract, LD50 was determined and high-performance liquid chromatography (HPLC) was employed to specify the type and the rate of the active ingredients of the extract. Then, 36 male Wistar rats were randomly assigned into six groups (n=6). The first group was only administered with normal saline and the second group only received ethyl alcohol 6 mL/kg/day·BW. The third and the fourth groups received ethyl alcohol 6 mL/kg/day·BW plus 167.5 and 335 mg/kg/day·BW saffron petal extract for 8 weeks. The fifth and the sixth groups received ethyl alcohol 6 mL/kg/day·BW for the first 8 weeks and were subsequently gavage fed on saffron extract for 167.5 and 335 mg/kg/day·BW, respectively, during the next 8 weeks. In the beginning and after the termination of the treatment, blood samples were collected from all rats.Results: The LD50 of the extract was about 670 mg/kg. The HPLC results indicated that the extract contains important antioxidant ingredients. At the end of the study, the serum concentration of the inflammatory indices, renal enzymes, and hepatic enzymes experienced a significant reduction in all of the intervened groups compared to the negative control group (minimum significant difference: P<0.05) except for the treatment group 1.Conclusion: Based on the current results, the extract has a protective effect in a dosage-dependent way and greater protective roles were documented for higher dosages.Keywords: Crocus sativus L., alcohol, kidney, liver, ratAzizi MAbbasi NMohamadpour MBakhtiyari SAsadi SShirzadpour EAidy AMohamadpour MAmraei MDove Medical PressarticleCrocus Sativus L.AlcoholKidneyLiverRatPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 12, Pp 269-283 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Crocus Sativus L. Alcohol Kidney Liver Rat Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Crocus Sativus L. Alcohol Kidney Liver Rat Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Azizi M Abbasi N Mohamadpour M Bakhtiyari S Asadi S Shirzadpour E Aidy A Mohamadpour M Amraei M Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
description	Monireh Azizi,1 Naser Abbasi,2,3 Mojtaba Mohamadpour,4 Salar Bakhtiyari,5 Sirous Asadi,4 Ehsan Shirzadpour,5 Ali Aidy,2 Mahmoud Mohamadpour,5 Mansour Amraei2,6 1Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 2Biotechnology and Medicinal Plants Research Center, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 3Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 4Student Research Committee, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 5Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran; 6Department of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IranCorrespondence: Mansour AmraeiDepartment of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Banganjab Street, PO Box 6939177143, Ilam, IranTel +98 843 223 5724Fax +98 843 222 7136Email amraei.mansour@yahoo.comBackground: Studies have shown that consumption of high levels of alcohol causes many negative effects on the liver and kidneys where antioxidant ingredients can be a proper solution to reducing the resulting damages. So, the present study investigated the effect of hydroalcoholic extract of Crocus sativus L. (saffron) petal with antioxidant properties on the changes in inflammatory and enzymatic indices resulting from alcohol use in the male rats’ kidney and liver.Materials and methods: After preparing the extract, LD50 was determined and high-performance liquid chromatography (HPLC) was employed to specify the type and the rate of the active ingredients of the extract. Then, 36 male Wistar rats were randomly assigned into six groups (n=6). The first group was only administered with normal saline and the second group only received ethyl alcohol 6 mL/kg/day·BW. The third and the fourth groups received ethyl alcohol 6 mL/kg/day·BW plus 167.5 and 335 mg/kg/day·BW saffron petal extract for 8 weeks. The fifth and the sixth groups received ethyl alcohol 6 mL/kg/day·BW for the first 8 weeks and were subsequently gavage fed on saffron extract for 167.5 and 335 mg/kg/day·BW, respectively, during the next 8 weeks. In the beginning and after the termination of the treatment, blood samples were collected from all rats.Results: The LD50 of the extract was about 670 mg/kg. The HPLC results indicated that the extract contains important antioxidant ingredients. At the end of the study, the serum concentration of the inflammatory indices, renal enzymes, and hepatic enzymes experienced a significant reduction in all of the intervened groups compared to the negative control group (minimum significant difference: P<0.05) except for the treatment group 1.Conclusion: Based on the current results, the extract has a protective effect in a dosage-dependent way and greater protective roles were documented for higher dosages.Keywords: Crocus sativus L., alcohol, kidney, liver, rat
format	article
author	Azizi M Abbasi N Mohamadpour M Bakhtiyari S Asadi S Shirzadpour E Aidy A Mohamadpour M Amraei M
author_facet	Azizi M Abbasi N Mohamadpour M Bakhtiyari S Asadi S Shirzadpour E Aidy A Mohamadpour M Amraei M
author_sort	Azizi M
title	Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
title_short	Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
title_full	Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
title_fullStr	Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
title_full_unstemmed	Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
title_sort	investigating the effect of crocus sativus l. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/22cc5d7526e242029802695959bd536a
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Investigating the effect of Crocus sativus L. petal hydroalcoholic extract on inflammatory and enzymatic indices resulting from alcohol use in kidney and liver of male rats

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